Sample menu:

Macedonian Veterinary Review

logo

p-ISSN 1409-7621
e-ISSN 1857-7415

line
Co-publishing with:
line
 
De Gruyter
line
Membership
line
cope
line
crossref
line
linked
line
crosref1
line
ithenticate
line
 

Abstract / References


Linija
Original Scientific Article
Published 15 March 2017
 
access
 

Development of simple multiplex real-time PCR assays for foodborne pathogens detection and identification on LightCycler
Avo Karus1, Fabrizio Ceciliani2, Armand Sanches Bonastre3, Virge Karus1
1Department of Food Sciences and Food Technology, Institute of Veterinary Medicine
and Animal Science, Estonian University of Life Sciences, Kreutzwaldi 62, Tartu, Estonia
2Department of Veterinary Science and Public Health, University of Milan,via Celoria 10, 20133 Milan, Italy
3Universitat Autonoma de Barcelona, Campus de la UAB, Bellaterra, Spain

ABSTRACT
Most acute intestinal diseases are caused by food-borne pathogens. A fast and simple real-time PCR-based procedure for simultaneous detection of food contamination by any of the five food-borne pathogens: Campylobacter jejuni, Mycobacterium bovis, Enterobacter sakazaki, Shigella boydii, Clostridium perfrigens using multiplex EvaGreen real-time PCR for LightCycler was developed and evaluated. Real-time qPCR showed excellent sensitivity. Tm calling and Melting Curve Genotyping (MCG) were used for analysis of PCR product melting curves. The Melting Curve Genotyping option showed good performance for discrimination of positive samples containing DNA of single pathogen or pathogen mixtures from negative samples.
Key words: food-borne pathogens, multiplex real-time PCR, melting curve genotyping

Mac Vet Rev 2017; 40 (1): 53-58
   
[ PDF Free Article ] pdf Linija          
Available Online First: 3 January 2017
 
 
Linija
References
 
 
.
1.

Fleckenstein, JM., Bartels, SR., Drevets, PD., Bronze, MS., Drevets, DA. (2010). Infectious agents of food- and water-borne illnesses. Am J Med Sci, 340(3), 238-246.
https://doi.org/10.1097/MAJ.0b013e3181e99893

2.

Postollec, F., Falentin, H., Pavan, S., Combrisson, J., Sohier, D. (2011). Recent advances in quantitative PCR (qPCR) applications in food microbiology. Food Microbiol, 28(5), 848-861.
https://doi.org/10.1016/j.fm.2011.02.008
PMid:21569926

3.

Severgnini, M., Cremonesi, P., Consolandi, C., De Bellis, G., Castiglioni, B. (2011). Advances in DNA Microarray technology for the detection of foodborne pathogens. Food Bioproc Tech, 4, 936-953.
https://doi.org/10.1007/s11947-010-0430-5

4.

Jošić D., Petković J., Bunčić O., Lepšanović Z., Pivić R., Rašić Z., Katić V. (2016). Typing of indigenous Campylobacter spp. from Serbia by m-PCR and RAPD. Acta Veterinaria-Beograd 2016, 66 (2), 203-213.
https://doi.org/10.1515/acve-2016-0017

5.

Fukushima, H., Katsube, K., Hata, Y., Kishi, R., Fujiwara, S. (2007). Rapid separation and concentration of food-borne pathogens in food samples prior to quantification by viable-cell counting and real-time PCR. Appl Environ Microbiol, 73(1), 92-100.
https://doi.org/10.1128/AEM.01772-06
PMid:17056684 PMCid:PMC1797114

6.

Fukushima, H., Kawase, J., Etoh, Y., Sugama, K., Yashiro, S., Iida, N., Yamaguchi, K. (2010). Simultaneous screening of 24 target genes of foodborne pathogens in 35 foodborne outbreaks using multiplex real-time SYBR green PCR analysis. International Journal of Microbiology. Vol.12010, Article ID 864817, 18 pages.
https://doi.org/10.1155/2010/864817

7.

Zhao, X., Lin, C-W., Wang J., Oh, D.H. (2014). Advances in Rapid Detection Methods for Foodborne Pathogens. J.Microbiol.Biotechnol 24(3), 297-312.
https://doi.org/10.4014/jmb.1310.10013
PMid:24375418

8.

Binnicker MJ. (2015). Multiplex molecular panels for diagnosis of gastrointestinal infection: performance, result interpretation, and cost-effectiveness. J.Clin.Microbiol. 53:3723-3728. Doi:10.1128/JCM.02103-15.
https://doi.org/10.1128/JCM.02103-15

9.

Cremonesi, P.; Pisani, L. F.; Lecchi, C.; Ceciliani, F.; Martino, P.; Bonastre, A. S.; Karus, A.; Balzaretti, C.; Castiglioni, B. (2014). Development of 23 individual TaqMan® real-time PCR assays for identifying common foodborne pathogens using a single set of amplification conditions. Food Microbiol, 43(October 2014), 35 - 40.
https://doi.org/10.1016/j.fm.2014.04.007
PMid:24929880

10.

Amagliani, G., Omiccioli, E., Campo, A., Bruce, IJ., Brandi, G., Magnani, M. (2006). Development of a magnetic capture hybridization-PCR assay for Listeria monocytogenes direct detection in milk samples. J Appl Microbiol, 100(2), 375-383.
https://doi.org/10.1111/j.1365-2672.2005.02761.x
PMid:16430514

11.

Justé, A., Thomma, BP., Lievens, B. (2008). Recent advances in molecular techniques to study microbial communities in food-associated matrices and processes. Food Microbiol, 25(6), 745-761.
https://doi.org/10.1016/j.fm.2008.04.009
PMid:18620966

12.

Elizaquível, P., Aznar, R. (2008). A multiplex RTi-PCR reaction for simultaneous detection of Escherichia coli O157:H7, Salmonella spp. and Staphylococcus aureus on fresh, minimally processed vegetables. Food Microbiol, 25(5), 705-713.
https://doi.org/10.1016/j.fm.2008.03.002
PMid:18541170

13.

Kawasaki, S., Fratamico, PM., Horikoshi, N., Okada, Y., Takeshita, K., Sameshima, T., Kawamoto, S. (2010). Multiplex real-time polymerase chain reaction assay for simultaneous detection and quantification of Salmonella species, Listeria monocytogenes, and Escherichia coli O157:H7 in ground pork samples. Foodborne Pathog Dis, 7(5), 549-554.
https://doi.org/10.1089/fpd.2009.0465
PMid:20132032

14.

Omiccioli, E., Amagliani, G., Brandi, G., Magnani, M. (2009). A new platform for Real-Time PCR detection of Salmonella spp., Listeria monocytogenes and Escherichia coli O157 in milk. Food Microbiol, 26(6), 615-622.
https://doi.org/10.1016/j.fm.2009.04.008
PMid:19527837

15.

Suo, B., He, Y., Tu, SI., Shi, X. (2010). A multiplex real-time polymerase chain reaction for simultaneous detection of Salmonella spp., Escherichia coli O157, and Listeria monocytogenes in meat products. Foodborne Pathog Dis, 7(6), 619-628.
https://doi.org/10.1089/fpd.2009.0430
PMid:20113204

 
 
Linija

 

 

 

lc
cope This journal is a member of and subscribes to the principles of the Committee on Publication Ethics.
crossref
CrosCheck
lc
Creative Commons License
The all content of the Journal "Mac Vet Rev", except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 License.
iThenticate
lc