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Abstract / References


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Original Scientific Article
Published 15 March 2017
 
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Detection of Staphylococcus aureus among coagulase positive staphylococci from animal origin based on conventional and molecular methods
Nikolina Velizarova Rusenova1, Anton Georgiev Rusenov2
1Department of Veterinary Microbiology Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, Trakia University, Stara Zagora 6000, Bulgaria
1Department of Internal Diseases, Faculty of Veterinary Medicine, Trakia University, Stara Zagora 6000, Bulgaria

ABSTRACT
The present study aimed to detect Staphylococcus aureus (S. aureus) among other coagulase positive staphylococci from animal origin by using conventional methods (biochemical tests and latex agglutination) and a molecular method, based on the nuc gene, as the gold standard and to assess the usefulness of these methods. For this purpose, total of 344 staphylococcal isolates were collected and analysed. A total of 156 isolates suspicious for S. aureus were detected by a conventional biochemical method – 88 from cows, 18 from goats, 7 from pigs, 17 from poultry, 7 from rabbits and 19 from dogs. The majority of S. aureus strains gave typical biochemical reactions with the exception of 30 (19.2%) and 25 (16%) that were VP negative and weak positive in fermenting mannitol, respectively. Twelve strains were found to be non-haemolytic (7.7%) and four strains did not ferment trehalose (2.6%). Other staphylococci were identified as S. pseudintermedius (n = 103), S. hyicus (n = 23) and the rest were coagulase-negative staphylococci. Latex agglutination test resulted in rapid positive reactions with S. aureus with exception of 5 strains (3.2%) from cow mastitis milk. Positive agglutination reactions were also established with S. pseudintermedius, and S. hyicus. PCR confirmed all strains that were preliminary identified as S. aureus by amplification of 270 bp fragment of nuc gene specific for this species. The atypical reactions in certain strains established in this study have shown that the precise detection of S. aureus from animal origin should be done by combination of conventional and molecular methods.
Key words: Staphylococcus aureus, biochemical method, latex agglutination, nuc gene, animals

Mac Vet Rev 2017; 40 (1): 29-36
   
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Available Online First: 15 November 2016
 
 
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