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Macedonian Veterinary Review


p-ISSN 1409-7621
e-ISSN 1857-7415

Co-publishing with:
De Gruyter

Abstract / References

Short Communication
Published on: 15 March 2018

Effects of doxycycline treatment on hematological and blood biochemical parameters in dogs naturally infected with Ehrlichia canis
Elena Atanaskova Petrov1, Igor Ulcar2, Irena Celeska2, Ksenija Ilievska1, Pandorce Spasovska Trenkovska3, Todor Novakov1, Kiril Krstevski4, Toni Dovenski5, Jovana Stefanovska6
1University Veterinary Hospital, Faculty of Veterinary Medicine, University Ss. Cyril and Methodius, Lazar Pop Trajkov 5-7, 1000 Skopje, Macedonia
2Department of Pathophysiology, Faculty of Veterinary Medicine, University Ss. Cyril and Methodius, Lazar Pop Trajkov 5-7, 1000 Skopje, Macedonia
3Veterinary Clinic D-r Naletoski, 1244 No 36, 1000 Skopje, Macedonia
4Department of Infectious Diseases, Faculty of Veterinary Medicine, University Ss. Cyril and Methodius, Lazar Pop Trajkov 5-7, 1000 Skopje, Macedonia
5Department of Reproduction, Faculty of Veterinary Medicine, University Ss. Cyril and Methodius, Lazar Pop Trajkov 5-7, 1000 Skopje, Macedonia
6Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University Ss. Cyril and Methodius, Lazar Pop Trajkov 5-7, 1000 Skopje, Macedonia


Ehrlichia canis, the etiologic agent of canine monocytic ehrlichiosis (CME), is mainly transmitted by the brown dog tick Rhipicephalus sanguineus. Clinical signs of the disease can be various, depending on the stage of the disease. Typical changes in hematological and blood biochemical parameters are: severe thrombocytopenia, mild to marked non regenerative anaemia and hypoalbuminemia. In order to present the effects of the treatment protocol on several hematological and biochemistry parameters, 34 Ehrlichia canis positive dogs were compared before and after treatment with doxycycline 10mg/kg/day, in duration of four weeks. Besides the clinical sings and laboratory findings, diagnosis was confirmed by antibody tests (Bionote, Korea, AGROLABO S.p.A., Italy). The most common clinical sings were depression, lethargy, pyrexia, vomiting and anorexia. Hematological analyses were performed on the automatic hematology analyzer Exigo ЕоѕVet (Sweden), while biochemistry analyses (alanine aminotransferase, aspartate aminotransferase, urea, creatinine, albumin, total protein, globulin and alkaline phosphatase) were performed using the automatic analyzer ChemWell 2910 (Awareness Technology, INC, USA). Statistically significant difference (p<0.05) in hematology changes was present regarding the red blood cells count, platelet count, hematocrit and hemoglobin before and after treatment. Hypoalbuminaemia (Mean 19.21 ±4.96 g/l) was the only serum biochemistry parameter with significant change before and after treatment, as well. Treatment with doxycycline in patients with E.canis resulted in significant increase of hematology parameters (red blood cells, hemoglobin, haematocrit and platelets), as well as albumins in serum.
Key words: Ehrlichia canis, doxycycline, clinical sings, hematology, biochemical parameters

Mac Vet Rev 2018; 41 (1): 99-105
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Available Online First: 29 January 2018

1. Baneth, G., Harrus, S., Ohnona, F. S., Schlesinger, Y. (2009). Longitudinal quantification of Ehrlichia canis in experimental infection with comparison to natural infection. Vet Microbiol. 136 (3): 321-325.

2. Donatien, A., Lestoquard, F. (1935). Existence en Algerie d'une rickettsia du chien. Bull Soc Pathol Exot. 28, 418–419.

3. Kontos, V.I., Athanasiou, L.V., Bitschava D. (1998). Serological study of erhlichiosis in dogs with clinical leishmaniasis. J Hellenic Vet Med Soc. 171-174.

4. Hamel, D., Silaghi, C., Knaus, M., Visser, M., Kusi, I., Rapti, D., Pfister, K. (2009). Detection of Babesia canis subspecies and other arthropod-borne diseases in dogs from Tirana, Albania. Wien Klin Wochenschr. 121 (3): 42-45.

5. Potkonjak, A., Savic, S., Jurisic, A., Petrovic, A., Suvajdzic, L., Lako, B., Novakovic, Z. (2013). Seroepidemiological research of canine monocytic ehrlichiosis in the autonomous province of Vojvodina, Serbia. Acta Sci Vet. 41 (1106): 1-8.

6. Tsachev, I., Papadogiannakis, E. I., Kontos, V., Zarkov, I., Petrov, V., Pelagic, V. (2006). Seroprevalence of Ehrlichia canis infection among privately-owned dogs in northern Bulgaria (In Greek and English). J Hellenic Vet Med Soc. 57 (3): 212-216.

7. Stefanovska, J., Kochevsk,i Z., Iskra, C., Farkas, R., Naletoski, I., Mrenoshki, S., Mitrov, D., Pendovski, L. (2012). Serological evidence of Ehrlichia canis and Leishmania infantum co-infection in naturally exposed dogs on the territory of Republic of Macedonia. Proceedings of the 3rd International Scientific Meeting Days of Veterinary Medicine, September, 2-4, (pp. 38-39), Ohrid, Macedonia.

8. Waner, T., Keysary, A., Bark, H., Sharabani, E., Harrus, S. (1999). Canine monocytic ehrlichiosis-an overview. Isr J Vet Med. 54 (4): 103-107.

9. Mylonakis, M. E., Theodorou, K. N. (2017). Canine monocytic ehrlichiosis: An update on diagnosis and treatment. Acta Vet (Beogr). 67 (3): 299-317.

10. Waner, T., Harrus, S. (2013). Canine monocytic ehrlichiosis-from pathology to clinical manifestations. Isr J Vet Med. 68 (1): 12-18.

11. Neer, T.M., Breitschwerdt, E.B., Greene, R.T., Lappin, M.R. (2002). Consensus statement on ehrlichial disease of small animals from the infectious disease study group of the ACVIM. J Vet Intern Med. 16, 309-315.

12. Woody, B. J., Hoskins, J. D. (1991). Ehrlichial diseases of dogs. Vet Clin N Am Small Anim Pract. 21, 75–98.

13. Griffin, M.O., Fricovsky, E., Ceballos, G., Villarreal, F.J. (2010). Tetracyclines: A pleiotropic family of compounds with promising therapeutic properties. Am J Physiol Cell Physiol. 299, 539-548.
PMid:20592239 PMCid:PMC2944325

14. Bartsch, R.C., Greene, R.T. (1996). Post-therapy antibody titers in dogs with ehrlichiosis: follow-up study on 68 patients treated primarily with tetracycline and/or doxycycline. J Vet Intern Med. 10, 271–274.

15. Wen, B., Rikihisa, Y., Mott, J.M., Greene, R., Kim, H.Y., Zhi, N., Couto, G.C., Unver, A. Bartsch, R. (1997). Comparison of nested PCR with immunofluorescent-antibody assay for detection of Ehrlichia canis infection in dogs treated with doxycycline. J Clin Microbiol. 35 (7): 1852-1855.
PMid:9196207 PMCid:PMC229855

16. Iqbal, Z., Rikihisa, Y. (1994). Reisolation of Ehrlichia canis from blood and tissues of dogs after doxycycline treatment. J Clin Microbiol. 32, 1644– 1649.
PMid:7929751 PMCid:PMC263749

17. Harrus, S., Kenny, M., Miara, L., Aizenberg, I., Waner, T. Shaw, S. (2004). Comparison of simultaneous splenic sample PCR with blood sample PCR for diagnosis and treatment of experimental Ehrlichia canis infection. Antimicrob Chemoth. 48, 4488-4490.
PMid:15504892 PMCid:PMC525421

18. Schaefer, J. J., Kahn, J., Needham, G. R., Rikihisa, Y., Ewing, S. A., Stich, R. W. (2008). Antibiotic clearance of Ehrlichia canis from dogs infected by intravenous inoculation of carrier blood. Ann N Y Acad Sci. 1149, 263– 269.
PMid:19120226 PMCid:PMC3052985

19. Breitschwerdt, E. B., Hegarty, B. C., Hancock, S. I. (1998). Doxycycline hyclate treatment of experimental canine ehrlichiosis followed by challenge inoculation with two Ehrlichia canis strains. Antimicrob Agents Chemother. 42, 362–368.
PMid:9527787 PMCid:PMC105415

20. Troy, G. C., Forrester, S. D. (1990). Ehrlichia canis, E. equi, and E. Risticii infections. In: Greene CE (Ed.), Infectious Diseases of the Dog and Cat (pp. 404–414). Philadelphia, PA: WB Saunders.

21. Sainz, A., Tesouro, M. A., Amusategui, I., Rodriguez, F., Mazzucchelli, F., Rodriguez, M. (2000). Prospective comparative study of 3 treatment protocols using doxycycline or imidocarb dipropionate in dogs with naturally occurring ehrlichiosis. J Vet Intern Med. 14 (2): 134-139.

22. Spasojević-Kosić, L., Savić, S., Potkonjak, A., Vračar, V. (2015). Retrospective analysis of clinical and laboratory findings in hunting dogs with serologic reactions to tick-borne pathogens (Anaplasma phagocytophilum, Borrelia burgdorferi, Babesia canis, Ehrlichia canis, Ricketsia conorii). Vet glas. 69 (3-4): 219-232.

23. Nakaghi, A. C. H., Machado, R. Z., Costa, M. T., André, M. R., Baldani, C. D. (2008). Canine ehrlichiosis: clinical, hematological, serological and molecular aspects. Ciênc Rural. 38 (3): 766-770.

24. Neer, T.M. (1995). Ehrlichiosis update. Proceedings of the 13th Annual Congress of the American College of Veterinary Internal Medicine. San Diego, California, United States of America. Proceedings. pp. 822-826.

25. Neer, T.M., Harrus, S. (2006). Canine monocytotropic ehrlichiosis and neorickettsiosis (E. canis, E. chaffeensis, E. ruminantium, N. sennetsu, and N. risticii infections). In: Greene, C.E. (Ed.), Infectious diseases of the dog and cat, Third Ed. Saunders Elsevier (pp. 203–216). St. Louis, MO, USA.

26. Villaescusa, A., García-Sancho, M., Rodríguez-Franco, F., Tesouro, M. Á., Sainz, Á. (2015). Effects of doxycycline on haematology, blood chemistry and peripheral blood lymphocyte subsets of healthy dogs and dogs naturally infected with Ehrlichia canis. Vet J. 204 (3): 263-268.

27. Webb, J. A., Allen, D. G., Abrams-Ogg, A. C., Gentry, P. A. (2006). Effects of doxycycline, amoxicillin, cephalexin, and enrofloxacin on hemostasis in healthy dogs. Am J Vet Res. 67 (4): 569-576.

28. Abeygunavardena, I., I. Kakoma, Smith, R. D. (1990). Pathophysiology of canine ehrlichiosis, In: J. C. Williams and I. Kakoma (Eds.), Ehrlichiosis, a vector-borne disease of animals and humans (pp. 78-92). Boston: Kluwer Academic Publishers.

29. de Castro, M. B., Machado, R. Z., de Aquino, L. P. C. T., Alessi, A. C., Costa, M. T. (2004). Experimental acute canine monocytic ehrlichiosis: clinicopathological and immunopathological findings. Vet Parasitol. 119 (1): 73-86.

30. Harrus, S., Kass, P. H., Klement, E., Waner, T. (1997). Canine monocytic ehrlichiosis: a retrospective study of 100 cases, and an epidemiological investigation of prognostic indicators for the disease. Vet Rec. 141 (14): 360-363.

31. Frank, J. R., Breitschwerdt, E. B. (1999). A retrospective study of ehrlichiosis in 62 dogs from North Carolina and Virginia. J Vet Intern Med. 13 (3): 194-201.

32. Mylonakis, M. E., M. Kritsepi‐Konstantinou, J. S. Dumler, P. P. V. P. Diniz, M. J. Day, V. I. Siarkou, E. B. Breitschwerdt, V. Psychas, T. Petanides, A. F. Koutinas. (2010). Severe hepatitis associated with acute Ehrlichia canis infection in a dog. J Vet Intern Med. 24 (3): 633-638.





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