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Macedonian Veterinary Review


p-ISSN 1409-7621
e-ISSN 1857-7415

Co-publishing with:
De Gruyter

Abstract / References

Review Article

Effect of gum arabic on stallion sperm survival during cold storage and post freezing
Mohamed Ali1, Musa M. Musa2, Sulaiman Alfadul3, K. Al-Sobayel1
1Animal Production and Breeding, Faculty of Agriculture and Veterinary Medicine, Qassim University, Qassim State, Saudi Arabia
2Department of Chemistry, King Fahd University of Petroleum & Minerals Dhahran 31261, Saudi Arabia
3King Abdulaziz City for Science and Technology P.O.Box 6086, Riyadh 11442, Saudi Arabia


This study is aimed at investigating effects of supplementation of stallion’ semen extender with various concentrations of Gum Arabic (GA) versus egg yolk (EY) on viscosity, sperm motility and survival during cooling and freezing. Physical sperm characteristics; i.e. curvilinear velocity (VCL), straight line velocity (VSL), average path velocity (VAP), linearity (LIN) and straightness index (STR) were evaluated. Based on the sperm velocity (velocity of the average path), individual spermatozoons were classified into two major groups; i.e., progressively motile (>45 μm/sec) and immotile (0-45 μm/sec) spermatozoa. Addition of 3, 9 or 15% of GA to HF-20 extender resulted in linear decreases in VCL, VSL and VAP and a decrease in the percentage of progressively motile spermatozoa. Dilution of horse semen samples with high viscosityextenders (i.e., high percentage of GA) decreased the VCL, VSL and VAP in fresh and chilled semen. Freezing semen in high viscosity-extenders reduced percentage of progressively motile spermatozoa compared with those of low viscosity-extenders. In refrigerated and frozen semen samples, the extender containing 15% GA had detrimental effects on the percentage of progressively motile sperm cells and velocity of progressive motile sperm. Moreover, cooling sperm in extenders containing 9 or 15% of GA for 72 hours resulted in complete motility cessation. In conclusion, GA could replace EY in stallion semen
extenders at a level of 3% to maintain the physical and biological characteristics of cold and frozen semen.
Key words: sperm characteristics, stallion, freezing, gum arabic, ISAS

Mac Vet Rev 2017; 41 (1): i-xi
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Available Online First: 19 November 2017

1. Wall, R.J., Foote, R.H. (1999). Fertility of bull sperm frozen and stored in clarified egg yolk-Tris-glycerol extender. J Dairy Sci. 82, 817-21.

2. Diégo, M., Djemil B., Lamia A., Alberto N., Sandrine D., Daniel T. (2013). Preliminary results: the advantages of low-density lipoproteins for the cryopreservation of equine semen. J Equine Vet Sci. 33, 1068-1075.

3. Kampshmidt, R.F., Mayer, D.T., Herman, H.A. (1953). Lipid and lipoprotein constituents of egg yolk in the resistance and storage of bull spermatozoa. J Dairy Sci. 36, 733-742.

4. Van Wagtendonk-de Leeuw, A.M., Haring, R.M., Kaal-Lansbergen, L.M.T.E., Den Daas, J.H.G. (2000). Fertility results using bovine semen cryopreserved with extenders based on egg yolk and soybean extract. Theriogenology 54, 57-67.

5. Hirai, M.,Cerbito, W.A.,Wijayagunawardane, M.P.B., Braun, J.,Leidl, W., Ohosak, Q.K., Matsuzawa, T., Miyazawa, K., Sato, K. (1997). The effect of viscosity of semen diluents on motility of bull spermatozoa. Theriogenology 47, 1463-1478.

6. Vasan, S.S. (2011). Semen analysis and sperm function tests: how much to test? Indian J Urol. 27, 41-48.
PMid:21716889 PMCid:PMC3114587

7. Mortimer, S.T., van der Horst, G., Mortimer, D. (2015). The future of computer‑aided sperm analysis. Asian J Androl. 17, 545–553.
PMid:25926614 PMCid:PMC4492043

8. Turner, T.T., Reich, G.W. (1985). Cauda epididymidal sperm motility: A comparison among five species. Biol Reprod. 32, 120-128.

9. Mortimer, D., Christopher, L.R., Barratt L., Bjorndahl, C., de Jager, A., Jequier, M., Charles, H.M. (2013). What should it take to describe a substance or product as 'sperm-safe'. Hum Reprod Update. 19 (Suppl 1): i1-45.

10. Nishikawa, Y. (1975). Studies on the preservation of raw and frozen semen. J Reprod Fertil. 23, 99-104.

11. Neild, D.M., Chaves, M.G., Flores, M., Mora, N., Beconi, M., Agüero, A. (1999). Hypoosmotic test in equine spermatozoa. Theriogenology 51(4): 721-727.

12. Hafez, E.S.E. (1993). Reproduction in Farm Animals. In: E.S.E.Hafez, Lea and Febiger (Eds.), (pp. 330-343). Philadelphia

13. Evans, G., Maxwell, W.M.C. (1987). Handling and examination semen. In: Maxwell, W.M.C. (Ed.), Salamon's Artificial Insemination of Sheep and Goat. (pp. 93-106). Butterworths, Sydney.

14. Petrie, A., Watson, P. (2002). Statistics for veterinary and animal science. Chapter 8 and 11. (pp. 90-138). Blackwell science

15. Hunter, R.H.F., Coy, P., Gadea, J., Rath, D. (2011). Considerations of viscosity in the preliminaries to mammalian fertilization. J Assist Reprod Genet. 28, 191–197.
PMid:21234667 PMCid:PMC3082651

16. Chian, R., Quinn, P. (2010). Cryoprotectant; In: Fertility cryopreservation. (pp. 24-38). Cambridge University Press.

17. Hyun, N., Chandsawangbhuwana, C., Zhu, Q., Shi, L.Z., Yang-Wong, C.,Berns, M.W. (2012). Effects of viscosity on sperm motility studied with optical tweezers. J Biomed Opt. 17, 1-6.

18. Suárez, S.S., Dai, X. (1992). Hyperactivation enhances mouse sperm capacity for penetrating viscoelastic media. Biol Reprod. 46, 686-691.

19. Amann, R.P., Graham, J.K. (1993). Spermatozoa functional. In: Mckinnon, A.O.; Voss, J.L. (Eds.) Equine reproduction. 1.ed. (pp. 715-745). Philadelphia: Lea and Febiger

20. Januskauskas, A., Rodriguez-Martinez, H. (1995). Assessment of sperm viability by measurement of ATP, membrane integrity and motility in frozen/thawed bull semen. Acta Vet Scandnavica. 36, 571-574.

21. Kirkman-Brown, J.C., Smith, D.J. (2011). Sperm motility: Is viscosity fundamental to progress? Mol Hum Reprod. 17, 539–544.

22. Woolley, D.M. (2003). Motility of spermatozoa at surfaces. Reproduction 126, 259–270.

23. Ford, W.C.L. (2006). Glycolysis and sperm motility does a spoonful of sugar help the flagellum go round. Hum Reprod Update. 112, 269 – 274.

24. Storey, B.T. (2008). Mammalian spermmetabolism oxygen and sugar, friend and foe. Int J Dev Biol. 52, 427– 437.

25. Gadelha, H., Gaffney, E.A., Smith, D.J., Kirkman-Brown J.C. (2010). Non-linear instability in flagellar dynamics:A novel modulation mechanism in sperm migration? J R Soc Interface. 7, 1689.
PMid:20462879 PMCid:PMC2988265

26. Province, C.A., Amann, R.P., Pickett, B.W., Squires, EL. (1984). Extenders for preservation of canine and equine spermatozoa at 5 °C. Theriogenology 22, 409-415.

27. Bruemmer, J.E., Coy, R.C., Squires, E.L., Graham, J.K. (2002). Effect of pyruvate on the function of stallion spermatozoa stored for up to 48 hours. J Anim Sci. 80, 12-18.

28. Rota, A.,Furzi, C.,Panzani, D., Camillo, F. (2004). Studies on motility and fertility of cooled stallion spermatozoa. Reprod Domest Anim. 39, 103-109.

29. Rota, A., Magelli, C., Panzani, D., Camillo, F. (2008). Effect of extender, centrifugation and removal of seminal plasma on cooled-preserved Amiata donkey spermatozoa. Theriogenology 69, 176-185.

30. Jeyendran, R.S., Van der Ven, H.H., Perez-Pelaez, M.,Crabo, B.G., Zaneveld, L.J. (1984). Development of an assay to assess the functional integrity of human sperm membrane and its relationship to other characteristics. J Reprod Fertil. 70, 219-228.





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