Sample menu:

Macedonian Veterinary Review

logo

p-ISSN 1409-7621
e-ISSN 1857-7415

line
Co-publishing with:
line
 
De Gruyter
line
Membership
line
cope
line
crossref
line
linked
line
crosref1
line
ithenticate
line
 

Abstract / References


Linija
Review Article
 
access
 

Effect of gum arabic on stallion sperm survival during cold storage and post freezing
Mohamed Ali1, Musa M. Musa2, Sulaiman Alfadul3, K. Al-Sobayel1
1Animal Production and Breeding, Faculty of Agriculture and Veterinary Medicine, Qassim University, Qassim State, Saudi Arabia
2Department of Chemistry, King Fahd University of Petroleum & Minerals Dhahran 31261, Saudi Arabia
3King Abdulaziz City for Science and Technology P.O.Box 6086, Riyadh 11442, Saudi Arabia

 

ABSTRACT
This study is aimed at investigating effects of supplementation of stallion’ semen extender with various concentrations of Gum Arabic (GA) versus egg yolk (EY) on viscosity, sperm motility and survival during cooling and freezing. Physical sperm characteristics; i.e. curvilinear velocity (VCL), straight line velocity (VSL), average path velocity (VAP), linearity (LIN) and straightness index (STR) were evaluated. Based on the sperm velocity (velocity of the average path), individual spermatozoons were classified into two major groups; i.e., progressively motile (>45 μm/sec) and immotile (0-45 μm/sec) spermatozoa. Addition of 3, 9 or 15% of GA to HF-20 extender resulted in linear decreases in VCL, VSL and VAP and a decrease in the percentage of progressively motile spermatozoa. Dilution of horse semen samples with high viscosityextenders (i.e., high percentage of GA) decreased the VCL, VSL and VAP in fresh and chilled semen. Freezing semen in high viscosity-extenders reduced percentage of progressively motile spermatozoa compared with those of low viscosity-extenders. In refrigerated and frozen semen samples, the extender containing 15% GA had detrimental effects on the percentage of progressively motile sperm cells and velocity of progressive motile sperm. Moreover, cooling sperm in extenders containing 9 or 15% of GA for 72 hours resulted in complete motility cessation. In conclusion, GA could replace EY in stallion semen
extenders at a level of 3% to maintain the physical and biological characteristics of cold and frozen semen.
Key words: sperm characteristics, stallion, freezing, gum arabic, ISAS

Mac Vet Rev 2017; 41 (1): i-xi
   
[ PDF Free Article ] pdf Linija          
Available Online First: 19 November 2017
 
 
Linija
References
 
 
 

1. Wall, R.J., Foote, R.H. (1999). Fertility of bull sperm frozen and stored in clarified egg yolk-Tris-glycerol extender. J Dairy Sci. 82, 817-21.
https://doi.org/10.3168/jds.S0022-0302(99)75301-4

2. Diégo, M., Djemil B., Lamia A., Alberto N., Sandrine D., Daniel T. (2013). Preliminary results: the advantages of low-density lipoproteins for the cryopreservation of equine semen. J Equine Vet Sci. 33, 1068-1075.
https://doi.org/10.1016/j.jevs.2013.04.004

3. Kampshmidt, R.F., Mayer, D.T., Herman, H.A. (1953). Lipid and lipoprotein constituents of egg yolk in the resistance and storage of bull spermatozoa. J Dairy Sci. 36, 733-742.
https://doi.org/10.3168/jds.S0022-0302(53)91553-7

4. Van Wagtendonk-de Leeuw, A.M., Haring, R.M., Kaal-Lansbergen, L.M.T.E., Den Daas, J.H.G. (2000). Fertility results using bovine semen cryopreserved with extenders based on egg yolk and soybean extract. Theriogenology 54, 57-67.
https://doi.org/10.1016/S0093-691X(00)00324-1

5. Hirai, M.,Cerbito, W.A.,Wijayagunawardane, M.P.B., Braun, J.,Leidl, W., Ohosak, Q.K., Matsuzawa, T., Miyazawa, K., Sato, K. (1997). The effect of viscosity of semen diluents on motility of bull spermatozoa. Theriogenology 47, 1463-1478.
https://doi.org/10.1016/S0093-691X(97)00136-2

6. Vasan, S.S. (2011). Semen analysis and sperm function tests: how much to test? Indian J Urol. 27, 41-48.
https://doi.org/10.4103/0970-1591.78424
PMid:21716889 PMCid:PMC3114587

7. Mortimer, S.T., van der Horst, G., Mortimer, D. (2015). The future of computer‑aided sperm analysis. Asian J Androl. 17, 545–553.
https://doi.org/10.4103/1008-682X.154312
PMid:25926614 PMCid:PMC4492043

8. Turner, T.T., Reich, G.W. (1985). Cauda epididymidal sperm motility: A comparison among five species. Biol Reprod. 32, 120-128.
https://doi.org/10.1095/biolreprod32.1.120
PMid:3971006

9. Mortimer, D., Christopher, L.R., Barratt L., Bjorndahl, C., de Jager, A., Jequier, M., Charles, H.M. (2013). What should it take to describe a substance or product as 'sperm-safe'. Hum Reprod Update. 19 (Suppl 1): i1-45.
https://doi.org/10.1093/humupd/dmt008
PMid:23552271

10. Nishikawa, Y. (1975). Studies on the preservation of raw and frozen semen. J Reprod Fertil. 23, 99-104.

11. Neild, D.M., Chaves, M.G., Flores, M., Mora, N., Beconi, M., Agüero, A. (1999). Hypoosmotic test in equine spermatozoa. Theriogenology 51(4): 721-727.
https://doi.org/10.1016/S0093-691X(99)00021-7

12. Hafez, E.S.E. (1993). Reproduction in Farm Animals. In: E.S.E.Hafez, Lea and Febiger (Eds.), (pp. 330-343). Philadelphia

13. Evans, G., Maxwell, W.M.C. (1987). Handling and examination semen. In: Maxwell, W.M.C. (Ed.), Salamon's Artificial Insemination of Sheep and Goat. (pp. 93-106). Butterworths, Sydney.

14. Petrie, A., Watson, P. (2002). Statistics for veterinary and animal science. Chapter 8 and 11. (pp. 90-138). Blackwell science

15. Hunter, R.H.F., Coy, P., Gadea, J., Rath, D. (2011). Considerations of viscosity in the preliminaries to mammalian fertilization. J Assist Reprod Genet. 28, 191–197.
https://doi.org/10.1007/s10815-010-9531-3
PMid:21234667 PMCid:PMC3082651

16. Chian, R., Quinn, P. (2010). Cryoprotectant; In: Fertility cryopreservation. (pp. 24-38). Cambridge University Press.

17. Hyun, N., Chandsawangbhuwana, C., Zhu, Q., Shi, L.Z., Yang-Wong, C.,Berns, M.W. (2012). Effects of viscosity on sperm motility studied with optical tweezers. J Biomed Opt. 17, 1-6.
https://doi.org/10.1117/1.JBO.17.2.025005
PMid:22463031

18. Suárez, S.S., Dai, X. (1992). Hyperactivation enhances mouse sperm capacity for penetrating viscoelastic media. Biol Reprod. 46, 686-691.
https://doi.org/10.1095/biolreprod46.4.686
PMid:1576267

19. Amann, R.P., Graham, J.K. (1993). Spermatozoa functional. In: Mckinnon, A.O.; Voss, J.L. (Eds.) Equine reproduction. 1.ed. (pp. 715-745). Philadelphia: Lea and Febiger

20. Januskauskas, A., Rodriguez-Martinez, H. (1995). Assessment of sperm viability by measurement of ATP, membrane integrity and motility in frozen/thawed bull semen. Acta Vet Scandnavica. 36, 571-574.

21. Kirkman-Brown, J.C., Smith, D.J. (2011). Sperm motility: Is viscosity fundamental to progress? Mol Hum Reprod. 17, 539–544.
https://doi.org/10.1093/molehr/gar043
PMid:21653751

22. Woolley, D.M. (2003). Motility of spermatozoa at surfaces. Reproduction 126, 259–270.
https://doi.org/10.1530/rep.0.1260259
PMid:12887282

23. Ford, W.C.L. (2006). Glycolysis and sperm motility does a spoonful of sugar help the flagellum go round. Hum Reprod Update. 112, 269 – 274.
https://doi.org/10.1093/humupd/dmi053
PMid:16407453

24. Storey, B.T. (2008). Mammalian spermmetabolism oxygen and sugar, friend and foe. Int J Dev Biol. 52, 427– 437.
https://doi.org/10.1387/ijdb.072522bs
PMid:18649255

25. Gadelha, H., Gaffney, E.A., Smith, D.J., Kirkman-Brown J.C. (2010). Non-linear instability in flagellar dynamics:A novel modulation mechanism in sperm migration? J R Soc Interface. 7, 1689.
https://doi.org/10.1098/rsif.2010.0136
PMid:20462879 PMCid:PMC2988265

26. Province, C.A., Amann, R.P., Pickett, B.W., Squires, EL. (1984). Extenders for preservation of canine and equine spermatozoa at 5 °C. Theriogenology 22, 409-415.
https://doi.org/10.1016/0093-691X(84)90461-8

27. Bruemmer, J.E., Coy, R.C., Squires, E.L., Graham, J.K. (2002). Effect of pyruvate on the function of stallion spermatozoa stored for up to 48 hours. J Anim Sci. 80, 12-18.
https://doi.org/10.2527/2002.80112x

28. Rota, A.,Furzi, C.,Panzani, D., Camillo, F. (2004). Studies on motility and fertility of cooled stallion spermatozoa. Reprod Domest Anim. 39, 103-109.
https://doi.org/10.1111/j.1439-0531.2004.00488.x
PMid:15065992

29. Rota, A., Magelli, C., Panzani, D., Camillo, F. (2008). Effect of extender, centrifugation and removal of seminal plasma on cooled-preserved Amiata donkey spermatozoa. Theriogenology 69, 176-185.
https://doi.org/10.1016/j.theriogenology.2007.09.003
PMid:17945340

30. Jeyendran, R.S., Van der Ven, H.H., Perez-Pelaez, M.,Crabo, B.G., Zaneveld, L.J. (1984). Development of an assay to assess the functional integrity of human sperm membrane and its relationship to other characteristics. J Reprod Fertil. 70, 219-228.
https://doi.org/10.1530/jrf.0.0700219
PMid:6694140

 
 
Linija

 

 

 

lc
cope This journal is a member of and subscribes to the principles of the Committee on Publication Ethics.
crossref
CrosCheck
lc
Creative Commons License
The all content of the Journal "Mac Vet Rev", except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 License.
iThenticate
lc