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Macedonian Veterinary Review


p-ISSN 1409-7621
e-ISSN 1857-7415

Co-publishing with:
De Gruyter

Abstract / References

Original Scientific Article
Published on: 15 March 2018

The role of myofibroblasts in granulomatous lymphadenitis in pigs naturally infected  with M. avium subsp. hominissuis
Vladimir Polaček1, Dejan Vidanović2, Biljana Božić1, Žolt Beckei3, Ivana Vučićević4, Jasna Prodanov-Radulović1 , Sanja Aleksić-Kovacević4
1Scientific Veterinary Institute “Novi Sad”, Rumenacki Put 20, 21000 Novi Sad, Serbia
2Veterinary Specialized Institute “Kraljevo”, Zicka 34, 36000 Kraljevo, Serbia
3Department of Animal Husbandry, Faculty of Veterinary Medicine, Belgrade University, Bulevar Oslobodenja 18, 11000 Belgrade, Serbia
4Department of Pathology, Faculty of Veterinary Medicine, Belgrade University, Bulevar Oslobodenja 18, 11000 Belgrade, Serbia


The most important morphological characteristic of infections caused by M. avium subsp. hominissuis (MAH) is granuloma formation. The growth of mycobacteria is in accordance with anti-bacterial effector mechanisms of the host within granuloma. The most important cytokines for „orchestrating“the host defense are interferon γ (INF-γ), tumor necrosis factor α (TNF-α) and transforming growth factor β1 (TGF-β1). Myofibroblasts that make up a peripheral layer of granuloma largely express receptors for TGF-β1. This cytokine is believed to affect the induction of myofibroblast proliferation. The aim of this paper is to point out the importance of myofibroblasts in the formation and sustainability of granuloma during natural infection of pigs with M. avium subsp. hominissuis. Examinations have been performed on the samples of Lnn. jejunales, Lnn. ileocolici and Lnn. colici of 100 pigs with a positive tuberculin skin test. The molecular method confirmed the presence of a genome M. avium subsp. hominissuis. The microscopic examination of lymph node samples stained by the routine hematoxyilin-eosin (HE) method, showed the presence of granulomatous lymphadenitis. The method of double immunohistochemical staining revealed that myofibroblasts which express TGF-β1 receptor type I (TGF-β1RI) and α smooth muscle actin (α SMA) have an important role in the morphogenesis of granulomatous lymphadenitis in pigs infected with MAH.
Key words: Mycobacterium avium subsp. hominissuis, granuloma, myofibroblast, TGF-β1, TGF-β1RI

Mac Vet Rev 2018; 41 (1): 47-53
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Available Online First: 9 December 2017

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