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Macedonian Veterinary Review


p-ISSN 1409-7621
e-ISSN 1857-7415

Co-publishing with:
De Gruyter

Abstract / References

Review Article

The oocyte´s nucleolus precursor body: The globe for life
Michal Benc1,2, Lazo Pendovski3, Matej Murin4, Frantisek Strejcek4, Martin Morovic4, Radek Prochazka5, Jozef Laurincik1,5
1Department of Zoology and Anthropology, Faculty of Natural Sciences, Constantine the Philosopher University in Nitra, Nabrezie mladeze 91, 949 74 Nitra, Slovak Republic
2Institute of Animal Science, Pratelstvi 815, 104 00 Prague - Uhrineves, Czech Republic
3Ss. Cyril and Methodius University in Skopje, Lazar Pop - Trajkov 5-7, 1000 Skopje, Republic of Macedonia
4Department of Botany and Genetics, Faculty of Natural Sciences, Constantine the Philosopher University in Nitra, Nabrezie mladeze 91, 949 74 Nitra, Slovak Republic
5The Czech Academy of Sciences, Institute of Animal Physiology and Genetics, Rumburska 89, 277 21 Libechov, Czech Republic


The nucleolus is the cell organelle responsible for ribosome synthesis and, hence, for protein synthesis. In the mammalian oocyte, the nucleolus compacts into a dense sphere with no ribosome synthesis well in advance of ovulation. It seems, that this body is of utmost importance for the development of the embryo. It is unknown, however, how it exerts this essential function. During the last two decades, great attention has been paid to the study of nucleogenesis in oocytes and early embryos, with transcription of ribosomal DNA being evaluated as one of the criteria of normal development. In this review, we summarize some aspects of nucleolus transformation during oocyte growth, as well as during early embryonic development with possible impact on the quality of the embryos used in biomedical research. This knowledge in connection with further observations will substantially contribute to the development of new criteria suitable for evaluation of oocytes and embryos used in biomedical application.
Key words: nucleolus precursor body, oocyte, embryo, transcriptomics, epigenetics

Mac Vet Rev 2018; 41 (2): i-viii
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Available Online First: 31 March 2018

1. Hyttel, P., Fair, T., Callesen, H., Greve, T. (1997). Oocyte growth, capacitation and final maturation in cattle. Theriogenology 47, 23-32.

2. Fair, T., Hyttel, P., Greve, T., Boland, M. (1996). Nucleolus structure and transcriptional activity in relation to oocyte diameter in cattle. Mol. Reprod. Dev. 43, 503-512.<503::AID-MRD13>3.0.CO;2-#

3. Bjerregaard, B., Wrenzycki, C., Philimonenko, V. V., Hozak, P., Laurincik, J., Niemann, H., Motlik, J., Maddox-Hyttel, P. (2003). Regulation of ribosomal RNA synthesis during the final phases of porcine oocyte growth. Biol. Reprod. 70, 925-935.

4. Hyttel, P., Laurincik, J., Rosenkranz, C., Rath, D., Niemann, H., Ochs, R. L., Schellander, K. (2000). Nucleolar proteins and ultrastructure in pre-implantation porcine embryos developed in vivo. Biol. Reprod. 63, 1848-1856.

5. Laurincik, J., Thomsen, P.D., Hay-Schmidt, A., Avery, B., Greve, T., Ochs, R. L., Hyttel, P. (2000). Nucleolar proteins and nuclear ultrastructure in pre-implantation bovine embryos produced in vitro. Biol. Reprod. 62, 1024-1032.

6. Bjerregaard, B., Wrenzycki, C., Strejcek, F., Laurincik, J., Holm, P., Ochs, R. L., Rosenkranz, C., Callesen, H., Rath, D., Niemann, H., Maddox-Hyttel, P. (2004). Expression of nucleolar related proteins in porcine preimplantation embryos produced in vivo and in vitro. Biol. Reprod. 70, 867-876.

7. Maddox-Hyttel, P., Bjerregaard, B., Laurincik, J. (2005). Meiosis and embryo technology: renaissance of the nucleolus. Reprod Fertil Dev. 17, 3-14.

8. Maddox-Hyttel, P., Svarcova, O., Laurincik, J. (2007). Ribosomal RNA and nucleolar proteins from the oocyte are to some degree used for embryonic nucleolar formation in cattle and pig. Theriogenology 68, 63-70.

9. Kyogoku, H., Ogushi, S., Miyano, T., Fulka, J. Jr. (2011). Nucleoli from growing oocytes inhibit the maturation of enucleolated, full-grown oocytes in the pig. Mol. Reprod. Dev. 78, 426-435.

10. Ogushi, S., Palmieri, Ch., Fulka, H., Saitou, M., Miyano, T., Fulka, J. Jr. (2008). The maternal nucleolus is essential for early embryonic development in mammals, Science 319, 613-616.

11. Wachtler, F., Stahl, A. (1993). The nucleolus: A structural and functional interpretation. Micron 24, 473-505.

12. Biggiogera, M., Malatesta, M., Abolhassani-Dadras, S., Amalric, F., Rothblum, L. I., Fakan, S. (2001). Revealing the unseen: the organizer region of the nucleolus. J. Cell Sci. 114, 3199–3205.

13. Koberna, K., Malinsky, J., Pliss, A., Masata, M., Vecerova, J., Fialova, M., Bednar, J., Raska, I. (2002). Ribosomal genes in focus: new transcripts label the dense fibrillar components and form clusters indicative of "Christmas trees" in situ. J. Cell. Biol. 157, 743-748.
PMid:12034768 PMCid:PMC2173423

14. Hozak, P., Cook, P. R., Schofer, C., Mosgoeller, W., Wachtler, F. (1994). Site of transcription of ribosomal RNA and intranucleolar structure in HeLa cells. J. Cell Sci. 107, 639-648.

15. Boisvert, F. M., Van Koningsbruggen, S., Navascues, J., Lamond, A. I. (2007). The multifunctional nucleolus. Nature 8, 574-585.

16. Fulka, H., Fulka, J. Jr. (2010). Nucleolar transplantation in oocytes and zygotes: challenges for further research. Mol. Hum. Reprod. 16, 63-67.

17. Crozet, N., Motlik, J., Szollosi, D. (1981). Nucleolar fine structure and RNA synthesis in porcine oocytes during early stages of antrum formation. Biol. Cell. 41, 35-42.

18. Motlik, J., Crozet, N., Fulka, J. (1984). Meiotic competence in vitro of pig oocytes isolated from early antral follicles. J. Reprod. Fertil. 72, 323-328.

19. Kopecny, V., Biggiogera, M., Laurincik, J., Pivko, J., Grafenau, P., Martin, T.E., Luhrmann, R., Fu, X. D., Fakan, S. (1996). Fine structural cytochemical and immunocytochemical analysis of nucleic acids and ribonucleoprotein distribution in nuclei of pig oocytes and early preimplantation embryos. Chromosoma 104, 561-574.

20. Kopecny, V., Landa, V., Pavlok, A. (1995). Localization of nucleic acids in the nucleoli of oocytes and early embryos of mouse and hamster: an autoradiographic study. Mol Reprod. Dev. 41 (4): 449-458.

21. Bouniol-Baly, C., Hamraoui, L., Guibert, J., Beaujean, N., Szollosi, M. S., Debey, P. (1999). Differential transcriptional activity associated with chromatin configuration in fully grown mouse germinal vesicle oocytes. Biol. Reprod. 60, 580-587.

22. De La Fuente, R. (2006). Chromatin modifications in the germinal vesicle (GV) of mammalian oocytes. Dev. Biol. 292, 1-12.

23. Fulka, H., Novakova, Z., Mosko, T., Fulka, J. Jr. (2009). The inability of fully grown germinal vesicle stage oocyte cytoplasm to transcriptionally silence transferred transcribing nuclei. Histochem Cell Biol. 132, 457-468.

24. Andersen, J. S., Lam, Y. W., Leung, A. K. L., Ong, S., Lyon, C. E., Lamond, A. I., Mann, M. (2005). Nucleolar proteome dynamics. Nature 433, 77-82.

25. Ogushi, S., Yamagata, K., Obuse, C., Furuta, K., Wakayama, T., Matzuk, M. M., Saitou, M. (2017). Reconstitution of the oocyte nucleolus in mice through a single nucleolar protein, NPM2. J Cell Sci. 130, 2416-2429.

26. Shishova, K. V., Lavrentyeva, E. A., Dobrucki, J. W. and Zatsepina, O. V. (2015). Nucleolus-like bodies of fully-grown mouse oocytes contain key nucleolar proteins but are impoverished for rRNA. Dev. Biol. 397, 267-281.

27. Bai, B., Liu, H., Laiho, M. (2014). Small RNA expression and deep sequencing analyses of the nucleolus reveal the presence of nucleolus-associated microRNAs. FEBS Open Bio. 4, 441-449.
PMid:24918059 PMCid:PMC4050192

28. Bai, B., Yegnasubramanian, S., Wheelan, S. J., Laiho, M. (2014). RNA-Seq of the nucleolus reveals abundant SNORD44-derived small RNAs. PLoS One. 9(9): e107519. doi: 10.1371/journal.pone.0107519. eCollection

29. Probst, A. V., Okamoto, I., Casanova, M., El Marjou, F., Le Baccon, P., Almouzni, G. (2010). A strand-specific burst in transcription of pericentric satellites is required for chromocenter formation and early mouse development. Dev. Cell. 19, 625-638.

30. Casanova, M., Pasternak, M., El Marjou, F., Le Baccon, P., Probst, A. V., Almouzni, G. (2013). Heterochromatin reorganization during early mouse development requires a single-stranded noncoding transcript. Cell Rep. 26, 1156-1167.

31. Santenard, A., Ziegler-Birling, C., Koch, M., Tora, L., Bannister, A. J., Torres-Padilla, M.E. (2010). Heterochromatin formation in the mouse embryo requires critical residues of the histone variant H3.3. Nat. Cell Biol. 12, 853-862.
PMid:20676102 PMCid:PMC3701880

32. Aguirre-Lavin, T., Adenot, P., Bonnet-Garnier, A., Lehmann, G., Fleurot, R., Boulesteix, C., Debey, P., Beaujean, N. (2012). 3D-FISH analysis of embryonic nuclei in mouse highlights several abrupt changes of nuclear organization during preimplantation development. BMC Dev. Biol. 12, 12-30.
PMid:23095683 PMCid:PMC3517311

33. Kyogoku, H., Fulka, J. Jr., Wakayama, T., Miyano, T. (2014). De novo formation of nucleoli in developing mouse embryos originating from enucleolated zygotes. Development 141, 2255-2259.

34. Morovic, M., Strejcek, F., Fulka Jr., J., Hyttel, P., Laurincik, J. (2014). Proteomic disproportion of nucleoli in pig and mouse fully grown oocytes. European Biotechnology Congress 2014, J. Biotech., 185, Supplement, September 2014, Page 46.

35. Morovic, M., Strejcek, F., Nakagawa, S., Deshmukh, R. S., Murin, M., Benc, M., Fulka, H., Kyogoku, H., Pendovski, L., Fulka, J., Jr., Laurincik, J. (2017). Mouse oocytes nucleoli rescue embryonic development of porcine enucleolated oocytes, Zygote ahead of print.

36. Suh, N., Baehner, L., Moltzahn, F., Melton, C., Shenoy, A., Chen, J., Blelloch, R. (2010). MicroRNA function is globally suppressed in mouse oocytes and early embryos. Current Biol. 20, 271-277.
PMid:20116247 PMCid:PMC2872512

37. Li, M., Xia, Y., Gu, Y., Zhang, K., Lang, Q., Chen, L., Guan, J., Luo, Z., Chen, H., Li, Y., Li, Q., Li, X., Jian, A., Shuai, S., Wang, J., Zhu, Q., Zhou, X., Gao, X., Li, X. (2010). MicroRNAome of porcine pre-and postnatal development. PLOS one 5:e11541.
PMid:20634961 PMCid:PMC2902522

38. Niemann, H., Tina, X. C, King, E. A., Lee, R. S. F. (2008). Epigenetic reprogramming in embryonic and foetal development upon somatic cell nuclear transfer cloning. Reproduction 135, 151-163.

39. Benc, M., Strejcek, F., Murin, M., Morovic, M., Martinkova, S., Jettmarova, D., Pendovski, L., Fulka, J. Jr., Laurincik, J. (2017). Nucleologenesis and nucleolotransfer in mammalian oocytes: A review. Mac Vet Rev. 40 (2): 117-124.





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