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Original Scientific Article
Published on: 15 October 2018
 
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The evaluation of Brucella spp. isolation rates in ruminant abortion cases by using different selective media
Mustafa Sencer Karagul1, Serkan Ikiz2
1Kartepe Vocational School of Equine Science, Kocaeli University, 41080, Kocaeli, Turkey
2Department of Microbiology, Faculty of Veterinary Medicine, Istanbul University, 34320, Istanbul, Turkey

 

ABSTRACT
The aim of this study is to evaluate the success of Brucella spp. isolation in ruminant abortion cases by using different selective media. To this end, 58 samples from ruminant abortion cases were utilized. 4 selective media; namely, Farrell Medium (FM), CITA Medium (CM), Modified Thayer Martin (MTM) and Jones & Morgan (JM) were preferred for isolation. In addition to these, one medium with antibiotics was used to extend the range of the results. Suspensions prepared from organ and fetal stomach contents were inoculated to media plates and incubated at 37Co for 5-8 days in 5-10% CO2 condition. Conventional biotyping method was used to identify Brucella isolates within the level of species and biovar. MTM (67.2%) and Farrell (65.5%) outperformed the other media with regards to isolation rate. However, regarding the inhibition ability against contaminant microrganisms, Farrell (86.2%) and CITA (72%) have the highest and second highest percentages respectively. The media’s inhibition ability was examined in the samples in which Brucella spp. isolation occurred to be able to investigate the correlations between isolation and inhibition. Lower isolation percentage was observed in the samples in which the media displayed the lowest inhibition ability against contaminants. In this context, using two different selective media with high inhibition ability against contaminants may be recommended to enhance the isolation rate. Moreover, the components stimulating the growth of Brucella strains might be added to the media to obtain better results.
Key words: Biovar, Brucella spp., inhibition, isolation, selective medium

Mac Vet Rev 2018; 41 (2): 177-186
   
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Available Online First: 16 September 2018
 
 
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