Sample menu:

Macedonian Veterinary Review


p-ISSN 1409-7621
e-ISSN 1857-7415

Co-publishing with:
De Gruyter

Abstract / References

Original Scientific Article
Published on: 15 March 2019

Emperipolesis: Sternal and femoral microenvironment induces megakaryiocyte emperipolesis in the Wistar strain
Damir Suljević, Filip Filipić, Erna Islamagić
Department for Biology, Faculty of Science, University of Sarajevo, Zmaja od Bosne 33-35, 71 000 Sarajevo, Bosnia and Herzegovina


Emperipolesis is considered a physiological phenomena often present in various pathophysiological conditions, but its etiology is still unknown. In this study, we analyzed the number of megakaryocytes and the percentage of emperipoletic cells in the sternal and femoral bone marrow of Wistar rats. Five types in the thrombopoiesis lineage (megakaryoblasts, promegakaryocytes and megakaryocytes - acidophilic, basophilic and thrombocytogenic) were determined. Except for basophilic megakaryocytes, significant differences were found for number of thrombopoietic cells in the sternal and femoral bone marrow. A larger number of thrombocytogenic megakaryocytes were present in the sternal bone marrow. Emperipoletic cells were significantly present in the femoral compared to the sternal bone marrow. Emperipolesis was typical for lymphocytes and neutrophils individually, while emperipolesis with two or more cells within thrombopoietic cell was
also present (1-7 %) and significant differences between the sternal and femoral bone marrow were detected. Emperipolesis was found in all analysed rats and it most commonly occured within mature megakaryocytes and rarely megakaryoblasts, while it was not recorded in the promegakaryocytes. The high incidence of megakaryocytes with emperopolesis in rats could be a consequence of “normal” cell retention in the cytoplasm of megakaryocytes while passing blood cells to circulation or related to haematopoietic response due to high incidence of inbreeding.
Key words: bone marrow, emperipolesis, femur, megakaryocytes, Wistar strain

Mac Vet Rev 2019; 42 (1): 71-77
[ PDF Free Article ] pdf Linija          
Available Online First: 4 February 2019

    1. Gupta, N., Jadhav, K., Shah, V. (2017). Emperipolesis, entosis and cell cannibalism: Demystifying the cloud. J Oral Maxillofac Pathol. 21 (1):92–98.
    PMid:28479694 PMCid:PMC5406827

    2. Raja, H., Subramanyam, S.G., Govindaraj, S., Babu, M.K. (2011). A rare cause of massive lymphadenopathy. Indian J Surg Oncol. 2 (3): 212-214.
    PMid:22942615 PMCid:PMC3272174

    3. Sable, M.N., Sehgal, K., Gadage, V.S., Subramanian, P.G., Gujral, S. (2009). Megakaryocytic emperipolesis: A histological finding in myelodysplastic syndrome. Indian J Pathol Microbiol. 52, 599–600.

    4. Rastogi, V., Sharma, R., Misra, S.R., Yadav, L., Sharma, V. (2014). Emperipolesis – A Review. J Clin Diagn Res. 8 (12): ZM01–ZM02.

    5. Amita, K., Vijay Shankar, S., Abhishekh, M.G., Geethalakshmi, U. (2011). Emperipolesis in a case of adult T cell lymphoblastic lymphoma (mediastinal type) – Detected at FNAC and imprint cytology. Online J Health Allied Sci. 10, 11.

    6. Vemuganti, G.K., Naik, M.N., Honavar, S.G. (2008). Rosaidorfman disease of the orbit. J Hematol Oncol. 1, 7.
    PMid:18588698 PMCid:PMC2474646

    7. Lee, W.B., Erm, S.K., Kim, K.Y., Becker, R.P. (1999). Emperipolesis of erythroblasts within Kupffer cells during hepatic hemopoiesis in human fetus. Anat Rec. 256, 158–164.<158::AID-AR6>3.0.CO;2-0

    8. Dziecioł, J., Lemancewicz, D., Kłoczko, J., Bogusłowicz, W., Lebelt, A. (2001). Megakaryocytes emperipolesis in bone marrow of the patients with non-Hodgkin's lymphoma. Folia Histochem Cytobiol. 2 (39):142-143.

    9. Poppema, S. (1978). Sternberg-Reed cells with intracytoplasmic lymphocytes. Phagocytosis or emperipolesis? Virchows Arch A Pathol Anat Histol. 380, 355–359.

    10. Xia, P., Wang, S., Guo, Z., Yao, X. (2008). Emperipolesis, entosis and beyond: Dance with fate. Cell Res. 18, 705–707.

    11. Sierro, F., Tay, S.S., Warren, A., Le Couteur, D.G., McCaughan, G.W., Bowen, D.G., Bertolino, P. (2015). Suicidal emperipolesis: a process leading to cell-in-cell structures, T cell clearance and immune homeostasis. Curr Mol Med. 15 (9):819-827.

    12. Mansour, A., Abou-Ezzi, G., Sitnicka, E., Jacobsen, S.E.W., Wakkach, A., Blin-Wakkach, C. (2012). Osteoclasts promote the formation of hematopoietic stem cell niches in the bone marrow. J Exp Med. 209 (3):537–549.
    PMid:22351931 PMCid:PMC3302238

    13. Arai, F., Hirao, A., Ohmura, M., Sato, H., Matsuoka, S., Takubo, K., Ito, K., Koh, G.Y., Suda, T. (2004). Tie2/angiopoietin-1 signaling regulates hematopoietic stem cell quiescence in the bone marrow niche. Cell. 118 (2): 149–161.

    14. Kunisaki, Y., Bruns, I., Scheiermann, C., Ahmed, J., Pinho, S., Zhang, D., Mizoguchi, T., Wei, Q., Lucas, D., Ito, K., Mar, J.C., Bergman, A., Frenette, P.S. (2013). Nature. 502 (7473): 637–643.
    PMid:24107994 PMCid:PMC3821873

    15. Becker, R.P., De Bruyn, P.P. (1976). The transmural passage of blood cells into myeloid sinusoids and the entry of platelets into the sinusoidal circulation; a scanning electron microscopic investigation. Am J Anat. 145 (2):183–205.

    16. Heazlewood, S.Y., Neaves, R.J., Williams, B., Haylock, D.N., Adams, T.E., Nilsson, S.K. (2013). Megakaryocytes co-localise with hemopoietic stem cells and release cytokines that up-regulate stem cell proliferation. Stem Cell Res. 11 (2):782–792.

    17. Hartwig, J., Italiano, J. (2003). The birth of the platelet. J Thromb Haemost. 1 (7):1580–1586.

    18. Shivdasani, R.A., Fujiwara, Y., McDevitt, M.A., Orkin, S.H. (1997). A lineage-selective knockout establishes the critical role of transcription factor GATA-1 in megakaryocyte growth and platelet development. EMBO J. 16 (13): 3965–3973.
    PMid:9233806 PMCid:PMC1170020

    19. Rozman, C., Vives-Corrons, J.L. (1981). On the alleged diagnostic significance of megakaryocytic "phagocytosis" (emperipolesis). Br J Haematol. 48, 510.

    20. Tavassoli, M. (1986). Modulation of megakaryocyte emperipolesis by phlebotomy: Megakaryocytes as a component of marrow-blood barrier. Blood Cells. 12, 205-216.

    21. Centurione, L., Di Baldassarre, A., Zingariello, M., Bosco, D., Gatta, V., Rana, R.A., Langella, V., Di Virgilio, A., Vannucchi, A.M., Migliaccio, A.R. (2004). Increased and pathologic emperipolesis of neutrophils within megakaryocytes associated with marrow fibrosis in GATA-1(low) mice. Blood 104 (12):3573-3580.

    22. Lee, K.P. (1989). Emperipolesis of hematopoietic cells within megakaryocytes in bone marrow of the rat. Vet Pathol. 26, 473-478.

    23. Bobik, R., Dabrowski, Z. (1995). Emperipolesis of marrow cells within megakaryocytes in the bone marrow of sublethally irradiated mice. Ann Hematol. 70 (2):91-95.

    24. De Pasquale, A., Paterlini, P., Quaglino, D., Quaglino, D. (1985). Emperipolesis ofgranulocytes within megakaryocytes. Br J Haematol. 60, 384-386.

    25. Faree, M., Afzal, M. (2014). Evidence of inbreeding depression on height, weight, and body mass index: a population-based child cohort. Am J Hum Biol. 26 (6):784–795.

    26. van Den Brandt, J., Kovács, P., Klöting, I. (2000). Metabolic variability among disease-resistant inbred rat strains and in comparison with wild rats (Rattus norvegicus). Clin Exp Pharmacol Physiol. 27 (10):793-795.





cope This journal is a member of and subscribes to the principles of the Committee on Publication Ethics.
Creative Commons License
The all content of the Journal "Mac Vet Rev", except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 License.