Sample menu:

Macedonian Veterinary Review

logo

p-ISSN 1409-7621
e-ISSN 1857-7415

line
Co-publishing with:
line
 
De Gruyter
line
Membership
line
cope
line
crossref
line
linked
line
crosref1
line
ithenticate
line
 

Abstract / References


Linija
Original Scientific Article
 
access
 

Immunohistomorphometric changes of the pituitary gonadotropic cells after testosterone application in a rat model of the andropause
Nataša Ristić1, Vladimir Ajdžanović1, Dragana Petrović-Kosanović1, Marko Miler1, Gordana Ušćebrka2, Verica Milošević1
1Department of Cytology, Institute for Biological Research “Siniša Stanković”, University of Belgrade, Despot Stefan Blvd., 142, 11060 Belgrade, Serbia
2Department of Veterinary Medicine, Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, Novi Sad, Serbia

 

ABSTRACT
Andropause, the culminating phase of male ageing, is characterized by deregulation of the hypothalamic-pituitarygonadal axis and low circulating free testosterone. The aim of this study was to investigate the immunohistomorphometric characteristics of the pituitary gonadotropic i.e. follicle-stimulating hormone (FSH) and the luteinizing hormone (LH) producing cells after testosterone application in a rat model of the andropause. Middle-aged Wistar rats were divided into
orchidectomized (ORX; n=8) and testosterone treated orchidectomized (ORX+T; n=8) groups. Testosterone propionate (5 mg/kg b.m. /day) was administered for three weeks, while the ORX group received the vehicle alone. Immunohistochemically stained FSH and LH cells underwent morphometric and optical density-related analysis, while circulating concentrations of the sex steroids were measured by immunoassays. Serum concentrations of testosterone and estradiol were significantly (p<0.05) increased by 24 and 2.7 fold respectively, compared to the ORX group. The volume of FSH and LH cells was significantly (p<0.05) decreased by 51.3% and 56.6% respectively, in comparison with ORX rats. Relative volume density of FSH and LH cells was also significantly (p<0.05) decreased by 54.0% and 72.8% respectively, compared to the ORX group. Results related to the optical density of gonadotropic cells (reflecting their hormonal content) were in line with the morphometric findings i.e. this parameter of FSH and LH cells was significantly (p<0.05) decreased by 25.7% and 16.2% respectively, in comparison with ORX rats. Conclusion: In conclusion, applied testosterone increased the serum concentrations of sex steroids, as well as it decreased morphometric parameters and optical density of gonadotropic cells in ORX rats.
Key words: andropause; testosterone; gonadotropic cells; immunohistomorphometry; optical density

Mac Vet Rev 2019; 42 (1): i-ix
   
[ PDF Free Article ] pdf Linija          
Available Online First: 3 November 2018
 
 
Linija
References
 
 
 

1. Wespes, E., Schulman, C.C. (2002). Male andropause: myth, reality, and treatment. Int J Impot Res. Suppl 1, 93-98.
https://doi.org/10.1038/sj.ijir.3900798
PMid:11850741

 

2. Morales A. (2004). Andropause (or symptomatic late-onset hypogonadism): facts, fiction and controversies. Aging Male 7, 297-303.
https://doi.org/10.1080/13685530400016664
PMid:15799125

3. Chahal, H.S., Drake, W.M. (2007). The endocrine system and ageing. J Pathol. 211, 173-180.
https://doi.org/10.1002/path.2110
PMid:17200939

4. Jiang, M., Huhtaniemi, I. (2004). Polymorphisms in androgen and estrogen receptor genes: effects on male aging. Exp Gerontol. 39, 603-611.
https://doi.org/10.1016/j.exger.2004.06.017
PMid:15582276

5. Wang, X.J., Stocco, D.M. (2005). The decline in testosterone biosynthesis during male aging: A consequence of multiple alterations. Mol Cell Endocrinol. 238, 1-7.
https://doi.org/10.1016/j.mce.2005.04.009
PMid:15939533

6. Bonavera, J.J., Swerdloff, R.S., Leung, A., Lue, Y.H., Baravarian, S., Superlano, L., Sinha-Hikim, A.P., Wang, C. (1997). In the male brown-Norway (BN) male rat, reproductive aging is associated with decreased LH-pulse amplitude and area. J Androl.18, 359-365.
PMid:9283947

7. Gruenewald, D.A., Naai, M.A., Marck, B.T., Matsumoto, A.M. (2000). Age-related decrease in hypothalamic gonadotropin-releasing hormone (GnRH) gene expression, but not pituitary responsiveness to GnRH, in the male Brown Norway rat. J Androl. 21, 72-84.
PMid:10670522

8. Elmlinger, M.W., Dengler, T., Weinstock, C., Kuehnel, W. (2003). Endocrine alterations in the aging male. Clin Chem Lab Med. 41, 934-941.
https://doi.org/10.1515/CCLM.2003.142
PMid:12940521

9. Hermann, M., Untergasser, G., Rumpold, H., Berger, P. (2000). Aging of the male reproductive system. Exp Gerontol. 35, 1267-1279.
https://doi.org/10.1016/S0531-5565(00)00159-5

10. Beg, S., Al-Khoury, L., Cunningham, G.R. (2008). Testosterone replacement in men. Curr Opin Endocrinol Diabetes Obes. 15, 364-370.
https://doi.org/10.1097/MED.0b013e328305081a
PMid:18594278

11. Singh, P. (2013). Andropause: current concepts. Indian J Endocrinol Metab. 17, 621-629.
https://doi.org/10.4103/2230-8210.123552
PMid:24910824 PMCid:PMC4046605

12. Theodoraki, A., Bouloux, P.M. (2009). Testosterone therapy in men. Menopause Int. 15, 87-92.
https://doi.org/10.1258/mi.2009.009025
PMid:19465676

13. Kunelius, P., Lukkarinen, O., Hannuksela, M.L., Itkonen, O., Tapanainen, J.S. (2002). The effects of transdermal dihydrotestosterone in the aging male: a prospective, randomized, double blind study. J Clin Endocrinol Metab. 87, 1467-1472.
https://doi.org/10.1210/jcem.87.4.8138
PMid:11932266

14. Kitahara, S., Winters, S.J., Oshima, H., Troen, P. (1991). Effects of gonadal steroids on follicle-stimulating and luteinizing hormone secretion by pituitary cells from castrated and intact male rats. Biol Reprod. 44, 121-126.
https://doi.org/10.1095/biolreprod44.1.121
PMid:1901736

15. Okada, Y., Fujii, Y., Moore, J.P., Jr., Winters, S.J. (2003). Androgen receptors in gonadotrophs in pituitary cultures from adult male monkeys and rats. Endocrinology. 144, 267-273.
https://doi.org/10.1210/en.2002-220770
PMid:12488354

16. Ajdžanović, V., Šošić-Jurjević, B., Filipović, B., Trifunović, S., Brkić, D., Sekulić, M., Milošević, V. (2009). Genistein affects the morphology of pituitary ACTH cells and decreases circulating levels of ACTH and corticosterone in middle-aged male rats. Biol Res. 42, 13-23.

17. Ajdžanović V., Šošić-Jurjević, B., Filipović, B., Trifunović, S., Milošević V. (2011). Daidzein effects on ACTH cells: immunohistomorphometric and hormonal study in an animal model of the andropause. Histol Histopathol. 26, 1257-1264.
PMid:21870329

18. Ajdžanović, V., Medigović, I., Živanović, J., Šošić-Jurjević, B., Trifunović, S., Tanić, N., Milošević, V. (2014). Immuno-histomorphometric and –fluorescent characteristics of GH cells after treatment with genistein or daidzein in an animal model of andropause. Acta Vet (Beograd). 64, 93-104.
https://doi.org/10.2478/acve-2014-0010

19. Filipović, B., Šošić-Jurjević, B., Ajdžanović, V., Pantelić, J., Nestorović, N., Milošević, V., Sekulić, M. (2013). The effects of sex steroids on thyroid C cells and trabecular bone structure in the rat model of male osteoporosis. J Anat. 222, 313-320.
https://doi.org/10.1111/joa.12013
PMid:23171170 PMCid:PMC3582251

20. Sternberger, L.A., Hardy, P.H., Jr., Cuculis, J.J., Meyer, H.G. (1970). The unlabeled antibody enzyme method of immunohistochemistry: preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J Histochem Cytochem. 18, 315-333.
https://doi.org/10.1177/18.5.315
PMid:4192899

21. Medigović, I., Manojlović-Stojanoski, M., Trifunović, S., Ristić, N., Milošević, V., Zikić, D., Nestorović, N. (2012). Effects of genistein on gonadotropic cells in immature female rats. Acta Histochem. 114:270-5.
https://doi.org/10.1016/j.acthis.2011.06.003
PMid:21703666

22. Poole, M.C., Kornegay, 3rd W.D. (1982). Cellular distribution within the rat adenohypophysis: a morphometric study. Anat Rec. 204, 45-53.
https://doi.org/10.1002/ar.1092040107
PMid:7149283

23. Ruifrok, A.C., Johnston, D.A. (2001). Quantification of histochemical staining by color deconvolution. Anal Quant Cytol Histol. 23, 291-299.
PMid:11531144

24. Varghese, F., Bukhari, A.B., Malhotra, R., De, A. (2014). IHC profiler: an open source plugin for the quantitative evaluation and automated scoring of immunohistochemistry images of human tissue samples. PLoS One. 9:e96801.
https://doi.org/10.1371/journal.pone.0096801
PMid:24802416 PMCid:PMC4011881

25. Ajdžanović, V., Jarić, I., Živanović, J., Filipović, B., Ristić, N., Miler, M., Milošević, V. (2015). Testosterone application decreases the capacity for ACTH and corticosterone secretion in a rat model of the andropause. Acta Histochem. 117, 528-535.
https://doi.org/10.1016/j.acthis.2015.04.002
PMid:25940766

26. Ajdžanović, V., Jarić, I., Živanović, J., Filipović, B., Šošić-Jurjević, B., Ristić, N., Stanković, S., Milošević, V. (2016). Histological parameters of the adrenal cortex after testosterone application in a rat model of the andropause. Histol Histopathol. 31:1209-1220.
PMid:26951517

27. Banerjee, A., Anjum, S., Verma, R., Krishna, A. (2012). Alteration in expression of estrogen receptor isoforms alpha and beta, and aromatase in the testis and its relation with changes in nitric oxide during aging in mice. Steroids 77, 609-620.
https://doi.org/10.1016/j.steroids.2012.02.004
PMid:22366072

28. Arimura, A., Shino, M., de la Cruz, K.G., Rennels, E.G., Schally, A.V. (1976). Effect of active and passive immunization with luteinizing hormone-releasing hormone on serum luteinizing hormone and follicle stimulating hormone levels and the ultrastructure of the pituitary gonadotrophs in castrated male rats. Endocrinology 99, 291-303.
https://doi.org/10.1210/endo-99-1-291
PMid:780100

29. Lindzey, J., Wetsel, W.C., Couse, J.F., Stoker, T., Cooper, R., Korach, K.S. (1998). Effects of castration and chronic steroid treatments on hypothalamic gonadotropin-releasing hormone content and pituitary gonadotropins in male wild-type and estrogen receptor-alpha knockout mice. Endocrinology139, 4092-40101.
https://doi.org/10.1210/endo.139.10.6253
PMid:9751487

30. Mitchner, N.A., Garlick, C., Ben-Jonathan, N. (1998). Cellular distribution and gene regulation of estrogen receptors alpha and beta in the rat pituitary gland. Endocrinology 139, 3976-3983.
https://doi.org/10.1210/endo.139.9.6181
PMid:9724053

 
 
Linija

 

 

 

lc
cope This journal is a member of and subscribes to the principles of the Committee on Publication Ethics.
crossref
CrosCheck
lc
Creative Commons License
The all content of the Journal "Mac Vet Rev", except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 License.
iThenticate
lc