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Linija
Original Scientific Article
Published on: 15 March 2019
 
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Metabolic profile comparison between follicular fluid and serum in normal cows and those affected by ovarian cysts
Nora Mimoune1, 2, Rachid Kaidi2, 3, Mohammed Hocine Benaissa4, Mohamed Wail Bahouh1, Ratiba Baazizi1, Mohamed Yassine Azzouz2
1National High School of Veterinary Medicine, Bab-Ezzouar, Algiers, Algeria
2Institute of Veterinary Sciences, LBRA, University of Blida 1, PB 270, Soumaa, Blida, Algeria
3School of Veterinary Medicine and Science, University of Nottingham, Nottingham, Leicestershire, United Kingdom
4Scientific and Technical Research Centre for Arid Areas (CRSTRA), Biophysical Station, Touggourt, Algeria

 

ABSTRACT
The aim of this study was to carry out the metabolic profile comparison between follicular fluid and serum in normal cows and those affected by ovarian cysts (OC). After slaughtering, blood samples and follicular fluids from normal and cystic animals were collected and assayed using commercial kits to determine the concentrations of metabolites (glucose, total protein, total cholesterol, cortisol, triglycerides, urea, creatinine and insulin) and the liver enzymes activity. Data showed that OC were characterized by low levels of glucose, total protein, cholesterol and cortisol in cystic fluid, while urea concentrations were high compared to normal follicular fluid (P<0.001). On the other hand, serum assays of cystic animals revealed very low values of insulin and urea, whereas cortisol levels were relatively high in comparison with the serum of normal cows (P<0.001). Significant correlations between the serum and follicular fluid concentrations of normal cows were found for glucose (r=0.49), total cholesterol (r=0.31), cortisol (r=0.38) and total protein (r=0.63). The highest correlation was found for urea (r=0.86). On contrary, weak correlations were observed between metabolites concentrations in cystic fluid and in serum for normal and cystic cows. In conclusion, OC grow and persist in a metabolic environment, which differs from follicular fluid to blood. These changes may act together and/or separately to ensure the continuous development of OC. To understand a part of the mechanism, the authors propose a deep study about blood-follicle-barrier.
Key words: ovarian cyst, follicular fluid, serum, metabolic profile.

Mac Vet Rev 2019; 42 (1): 51-59
   
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Available Online First: 30 December 2018
 
 
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