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Macedonian Veterinary Review


p-ISSN 1409-7621
e-ISSN 1857-7415

Co-publishing with:
De Gruyter

Abstract / References

Original Scientific Article

Homologous seminal plasma and glutathione promote pre-capacitation motility and structural stability of cryopreserved ram spermatozoa
Martin Nikolovski1, Monika Dovenska1, Ksenija Ilievska1, Nikola Adamov1, Branko Atanasov1,Miroslav Radeski1, Daniela Kirovski2, Vladimir Petkov1, Toni Dovenski1
1Faculty of Veterinary Medicine - Skopje, Ss. Cyril and Methodius University in Skopje, 1000 Skopje, North Macedonia
2Faculty of Veterinary Medicine – Belgrade, University of Belgrade, Serbia


Reduced glutathione (GSH) and homologous ram seminal plasma (HSP), used as additives in cryopreserving (CP) media prior to freezing, showed conflicting results in retaining structural integrity and progressive motility in post-thawed ram spermatozoa. The aims of this research were: (1) to assess the effect of GSH and/or HSP supplementation via soybean-lecithin CP extender on cryopreserved ram spermatozoa viability, morphology and motility pattern; and (2) to assess the effect of incubation in the context of the previous aim. Quantitatively and qualitatively, homogenized and pooled ram ejaculates (N=10) were extended with one of the following extenders: control (C) – tris-based, GSH and HSP-free, experimental-1 (E1) – C + GSH 5 mM, experimental-2 (E2) – C + HSP 20 % and experimental-3 (E3) - GSH 5 mM + HSP 20 %. Following thawing, samples were taken at 0- and 3-hours from each group (n=10) and were assessed for spermatozoa viability, morphology, and motility pattern. C-0h samples yielded a spermatozoa population with low viability, altered head morphology and highly deviated motility pattern. E3-3h samples yielded spermatozoa with unaffected viability, head morphology and high progressive motility. In conclusion, E3 extender added to cryopreserved-thawed ram spermatozoa is most efficient in obtaining high viability, unaltered head morphology, and progressive motility.
Key words: ram, ejaculates, cryopreservation, homologous seminal plasma, reduced glutathione

Mac Vet Rev 2019; 42 (2): i-xi
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Available Online First: 5 September 2019

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