ORIGINAL_ARTICLE Hematological importance of pseudoeosinophilic granulocytes in acclimation of common carp (Cyprinus carpio Linnaeus, 1758) Adaptation mechanisms as response to water content, oxygen level and pollutants are very important and they can be interpreted by hematological analysis. The aim of this study was the analysis of hematological and immune adaptations of common carp (Cyprinus carpio Linnaeus, 1758) to thermal stress. All specimens were divided into a control and experimental group. The control group of fish was exposed to a constant water temperature of 10°C. We induced thermal stress in experimental fish by gradually heating water to 28°C, held for 30 minutes and then comparing the obtained results with the control fish. Short-term hyperthermia lead to an increase of the number of leukocytes, especially pseudoeosinophilic granulocytes and monocytes, while the number of neutrophils and lymphocytes was reduced. The analysis of the leukocyte number and differential blood count in the control group showed high individual variation of segmented granulocytes, monocytes and pseudoeosinophilic granulocytes. Statistically significant differences (p=0.00) were found for the white blood cells, nonsegmented neutrophils and pseudoeosinophils between the control and experimental group. The experimental group of males had an increased number of white blood cells, monocytes and pseudoeosinophils, where significant differences were found for nonsegmented and total neutrophils and also for pseudoeosinophils (p=0.00), lymphocytes (p=0.01) and monocytes (p=0.03). Females had an increased total number of white blood cells, lymphocytes, monocytes and pseudoeosinophils, while significant differences (p=0.00) were obtained in the number of white blood cells, nonsegmented and total neutrophils and pseudoeosinophils between the control and experimental group. Adaptation mechanisms in carp after water temperature heating are mostly reflected in the increase of pseudoeosinophils and the decrease of neutrophils. https://macvetrev.mk/Files/Article/2019/10.1515/macvetrev-2016-0091/macvetrev-2016-0091.pdf 2017-03-15T09:00:00 5 11 10.2478/macvetrev-2016-0091 Cyprinus pseudoeosinophilic granulocytes acclimation stress Damir Suljević false 1 Department of Biology, Faculty of Science, University of Sarajevo, Zmaja od Bosne 33-35, 71 000 Sarajevo, Bosnia and Herzegovina AUTHOR Adelaida Martinović-Jukić false 2 Department of Biology, Faculty of Science, University of Sarajevo, Zmaja od Bosne 33-35, 71 000 Sarajevo, Bosnia and Herzegovina AUTHOR Muhamed Fočak false 3 Department of Biology, Faculty of Science, University of Sarajevo, Zmaja od Bosne 33-35, 71 000 Sarajevo, Bosnia and Herzegovina AUTHOR Andi Alijagić andialijagic@gmail.com false 4 Department of Biology, Faculty of Science, University of Sarajevo, Zmaja od Bosne 33-35, 71 000 Sarajevo, Bosnia and Herzegovina LEAD_AUTHOR Dunja Rukavina false 5 Department of Morphology, Faculty of Veterinary Medicine, University of Sarajevo, Zmaja od Bosne 90, 71 000 Sarajevo, Bosnia and Herzegovina AUTHOR Amir Zahirović false 6 Department of Internal Diseases, Faculty of Veterinary Medicine, University of Sarajevo Zmaja od Bosne 90, 71 000 Sarajevo, Bosnia and Herzegovina AUTHOR Pickering AD, Endocrine-induced pathology in stressed salmonid fishFish Res 1993; 17: 35-50. 1 10.1016/0165-7836(93)90005-R Everly GS, Lating JM, A clinical guide to the treatment of the human stress response 2013; USA: Springer science & Business media; 250-286. 2 10.1007/978-1-4614-5538-7 Iwama GK, Morgan JD, Barton B.A, Simple field methods for monitoring stress and general condition of fishAqua Res 1995; 26: 4273-282. 3 10.1111/j.1365-2109.1995.tb00912.x Kazlauskiene N, Vosyliene MZ, Physiological state of Atlantic salmon (Salmo salar Linnaeus 1758) and sea trout (Salmo trutta trutta Linnaeus 1758.)Acta Zool Lith 2004; 14: 448-51. 4 Mitrašinović M, Suljević D, Hematological status of chub fish Leuciscus cephalus (Linnaeus 1758.) from Krupica and Zeljeznica riversVeterinaria 2009; 58: 1-263-76. 5 Kroupova H, Machova J, Svobodova Z, Nitrite influence on fish: a reviewVet Med-Czech 2005; 50: 461-471. 6 Ritossa FM, A new puffing pattern induced by a temperature shock and DNP in DrosophilaExp Biol 1962; 18: 571-573. 7 10.1007/bf02172188 Blank M, Khorkova O, Goodman R, Changes in polypeptide distribution stimulated by different levels of Em and thermal stressBiochem Bioenerg 1994; 17: 349-360. 8 10.1016/0302-4598(87)80045-4 Samali A, Cotter TG, Heat shock proteins increase resistance to apoptosisExp Cell Res 1996; 223: 1163-170. 9 10.1006/excr.1996.0070PMid:8635489 Samali A, Holmberg CI, Sistonen L, Orrenius S, Thermotolerance and cell death are distinct cellular responses to stress: dependence on heat shock proteinsFEBS Lett 1999; 461: 3306-310. 10 10.1016/S0014-5793(99)01486-6 Samali A, Orrenius S, Heat shock proteins: regulators of stress response and apoptosisCell Stress Chap 1998; 3: 4228-236.PMCid: PMC312968 11 Ellis AE, Leukocytes in fishJ Fish Biol 1977; 11: 453-491. 12 10.1111/j.1095-8649.1977.tb04140.x Tavares-Dias M, Barcellos JFM, Peripheral blood cells of the armored catfish Hoplosternum littorale Hancock: a morphological and cytochemistry studyBraz J Morphol Sci 2005; 22: 215-220. 13 Tavares-Dias M, Moraes FR, Hematologia de peixes teleosteosRibeirão Preto 2004; 1: 144- 14 Lin S, Hematopoiesis L.I. 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