ORIGINAL_ARTICLE This study investigated the effect of three intramuscular injections of Prostaglandin F2α (PGF2α) administered seven days apart on estrus responses and some reproductive hormonal profiles in post-pubertal gilts.Sixteen gilts were divided into groups A, B, C, and D, with four animals in each group. Group A, B and C gilts were treated with PGF2α injections intramuscularly at doses 5mg,7.5mg and 10mg per gilt respectively on Days 0, 6 and 13 of the experiment. Group D (Control) were treated with intramuscular injections of 2ml phosphate-buffered saline (PBS) instead. Serum samples were collected every 24 hours for four days after each PGF2α injection and estrus detection was conducted daily. Results showed that 100% of Group A came into estrus 96 hours after the second PGF2α injection,while 50% of Group B came into estrus after the first injection; with the other 50% exhibiting estrus following the 2nd injection. Only 50% of Group C displayed estrus signs and this was observed following the second PGF2α injection. Control gilts did not express behavioral estrus during this study, suggesting that PGF2α may have induced estrus in the treated animals. LH and estradiol concentrations were significantly different in Group A and B when compared to the control following the 2nd PGF2α injection. In all, it was observed that serum concentrations of LH, FSH and oestradiol-17β reached maximal levels just prior to expression of estrus, thus suggesting that the pattern of reproductive hormonal changes in PGF2α-synchronized gilts may be quite similar to those of naturally cycling in-estrus gilts. https://macvetrev.mk/Files/Article/2019/10.1515/macvetrev-2016-0092/macvetrev-2016-0092.pdf 2017-03-15T09:00:00 13 20 10.2478/macvetrev-2016-0092 estrus responses hormones gilts PGF2α Yusuf Abdulrasheed Alimi abdulrasheedyusuf@yahoo.com false 1 Department of Veterinary Surgery and Reproduction, University of Ibadan, 200284 Ibadan, Nigeria; Department of Veterinary Physiology and Biochemistry, University of Abuja, 902101 Abuja, Nigeria LEAD_AUTHOR Oluwafisayo Oluwadamilare Leigh false 2 Department of Veterinary Surgery and Reproduction, University of Ibadan, 200284 Ibadan, Nigeria AUTHOR Oluwatoyin Oluwasola Ajala false 3 Department of Veterinary Surgery and Reproduction, University of Ibadan, 200284 Ibadan, Nigeria AUTHOR John Olusoji Abiola false 4 Department of Veterinary Medicine, University of Ibadan, 200284, Ibadan, Nigeria AUTHOR Ziecik AJ, Biallowicz M, Kaczmarek M, Demianowicz W, Rioperez J, Wasielak M, Bogacki M, Influence of estrus synchronization of prepubertal gilts on embryo qualityJ. Reprod. Dev 2005; 51: 3379-384. PMid:15827382 1 10.1262/jrd.17008 Kirkwood RN, Pharmacological intervention in swine reproductionSwine Health Prod 1999; 7: 129-35. 2 De Rensis F, Peter A, The control of follicular dynamics by PGF2α GnRH, hCG and estrus synchronization in cattleReproduction in Domestic Animals 1999; 34: 49-59. 3 10.1111/j.1439-0531.1999.tb01383.x Menchaca A, Miller V, Gil J, Pinczak A, Laca M, Rubianes E, Prostaglandin F2α treatment is associated with timed artificial insemination in ewesReproduction in Domestic Animals 2004; 39: 352-355. PMid:15367269 4 10.1111/j.1439-0531.2004.00527.x De Rensis F, Lopez-Gatius F, Protocols for synchronizing estrus and ovulation in buffalo (Bubalus bubalis): A reviewTheriogenology 2007; 67: 209-216. PMid:17112577 5 10.1016/j.theriogenology.2006.09.039 Diehl JR, Day BN, Effect of prostaglandin F2α on luteal functiion in swineJ Anim Sci 1974; 39: 392-396. PMid:4845714 6 10.2527/jas1974.392392x Guthrie HD, Polge C, Luteal function and oestrus in gilts treated with a synthetic analogue of prostaglandin F2α (ICI79.939) at various times during the oestrous cycleJournal of Reproduction and Fertility 1976; 48: 423-425. PMid:1033285 7 10.1530/jrf.0.0480423 Manzoni C, Saleri R, Kirkwood RN, Macchi E, Mongkol T, De Rensis F, Double cloprostenol administration during mid-luteal phase of oestrous cycle does not modify the interoestrous interval in giltsItal J Anim Sci 2014; 13: 527-529. 8 Halfordm DM, Wettemannm RP, Turmanm EJ, Omtvedt IT, Luteal function in gilts after prostaglandin F2αJ Anim Sci 1975; 41: 1706-1710. 9 10.2527/jas1975.4161706x Estill CT, Britt JH, Gadsby JE, Repeated administration of prostaglandin F2α during the early luteal phase causes premature luteolysis in the pigBiol. Reprod 1993; 49: 181-185. PMid:8353186 10 10.1095/biolreprod49.1.181 Puglisi TA, Rampacek GB, Kraeling RR, Kiser TE, Corpus luteum susceptibility to prostaglandin F2α (PGF α) luteolysis in hysterectomized prepuberal and mature giltsProstaglandins 1979; 18: 2257-264. 11 10.1016/0090-6980(79)90111-4 Noguchi M, Kashiwa S, Itoh S, Okumura H, Kure K, Suzuki C, Yoshioka K, Reproductive hormone profiles in sows on estrus synchronization using estradiol dipropionate and prostaglandin F2α-analogue and the reproductive performance in female pigs on commercial farmsJ. Vet. Med. Sci 2013; 75: 3343-348. 12 10.1292/jvms.12-0022PMid:23131781 Soede NM, Langendijk P, Kemp B, Reproductive cycles in pigsAnim. Reprod. Sci 2011; 124: 251-258. PMid:21397415 13 10.1016/j.anireprosci.2011.02.025