ORIGINAL_ARTICLE The efficacy of intramuscular administration of Homidium chloride (Novidium®) and Isometamidium chloride (Sécuridium®) in Nigerian donkeys (Equus asinus) experimentally infected with T. b. brucei (Federe isolate) was investigated. Changes in haematological and serum biochemical indices were evaluated using clinical haematology and biochemistry methods. Red blood cell (RBC) count for the negative control group was significantly higher than for the positive control, Novidium® and Sécuridium®-treatment groups. Haemoglobin (Hb) concentration significantly reduced in the infected untreated group compared with other groups. Packed cell volume (PCV) was significantly different between negative and positive controls, and also between the infected untreated and treatment groups. There was significant reduction in platelet counts post-infection and post-treatment. Mean corpuscular volume (MCV) increased significantly in the treatment groups while mean corpuscular haemoglobin concentration (MCHC) significantly reduced only in the Sécuridium®-treatment group. Lymphocyte count for infected untreated was non-significantly higher than for the uninfected controls, but treatment with both trypanocides recorded further increases, which were higher compared with that of the uninfected group. Post infection and treatment, aspartate aminotransferase (AST) levels increased significantly. There were non-significant differences in electrolyte ion concentrations across the groups except for chloride ion which recorded a significant reduction in the Novidium®-treatment group. This experiment revealed that Nigerian donkeys infected with T. brucei brucei (Federe isolate) developed symptoms of trypanosomosis; anaemia, lymphocytosis and thrombocytopenia. Treatment with the trypanocides ameliorated effects of the infection, and results suggest that immunosuppression may not be a substantial clinical manifestation of T. brucei brucei (Federe isolate) trypanosomosis in Nigerian donkeys. https://macvetrev.mk/Files/Article/2019/10.1515/macvetrev-2017-0014/macvetrev-2017-0014.pdf 2017-03-15T09:00:00 73 82 10.2478/macvetrev-2017-0014 haematology Trypanosoma brucei brucei (Federe isolate) donkey homidium chloride isometamidium chloride Queen Nneka Oparah oparah.queen@gmail.com false 1 Department of Veterinary Medicine, Faculty of Veterinary Medicine, Ahmadu Bello University Zaria, Kaduna State, Nigeria LEAD_AUTHOR Anthony Bedu kojo Sackey false 2 Department of Veterinary Medicine, Faculty of Veterinary Medicine, Ahmadu Bello University Zaria, Kaduna State, Nigeria AUTHOR Idris Alao Lawal false 3 Department of Veterinary Parasitology and Entomology, Faculty of Veterinary Medicine, Ahmadu Bello University Zaria, Kaduna State, Nigeria AUTHOR Usman Shehu Abdullahi false 4 Department of Veterinary Medicine, Faculty of Veterinary Medicine, Ahmadu Bello University Zaria, Kaduna State, Nigeria AUTHOR Fielding D, Pearson RA, Donkeys, Mules and Horses in Tropical Agricultural Development 1991; Scotland: Proceedings of a Colloquium, September, 3–6, ISBN 0-907146066, Edinburgh University; 1 Assefa E, Abebe G, Drug resistance of Trypanosoma congolense in naturally infected donkeys in North Omo zone Southern EthiopiaVet Para 2001; 99: 261-271. 2 10.1016/S0304-4017(01)00489-7 Mesele F, Leta S, Prevalence rate of tsetse transmitted donkey trypanosomosis in Dale Wabera District, Western EthiopiaGlobal Vet 2010; 5: 3180-183. 3 Jatau ID, Lawal AI, Agbede RIS, Abdurrahman EM, Response of albino rats to experimental infection with primary and relapsed isolates of Trypanosoma evansiSahel Vet Sci 2012; 11: 149-57. 4 Ezebuiro OGC, Yohanna JA, Abdulya A, Osue HO, Abenga JN, Yakasai MA, Attahir A, Haematological changes following acute Trypanosoma brucei brucei infection in rabbitsCurr Res J Biol Sci 2012; 4: 4414-416. 5 Blench R, de Jode A, Gherzi E, Donkeys in Nigeria: history, distribution and productivity. 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