REVIEW_ARTICLE The nucleolus is the cell organelle responsible for ribosome synthesis and, hence, for protein synthesis. In the mammalian oocyte, the nucleolus compacts into a dense sphere with no ribosome synthesis well in advance of ovulation. It seems, that this body is of utmost importance for the development of the embryo. It is unknown, however, how it exerts this essential function. During the last two decades, great attention has been paid to the study of nucleogenesis in oocytes and early embryos, with transcription of ribosomal DNA being evaluated as one of the criteria of normal development. In this review, we summarize some aspects of nucleolus transformation during oocyte growth, as well as during early embryonic development with possible impact on the quality of the embryos used in biomedical research. This knowledge in connection with further observations will substantially contribute to the development of new criteria suitable for evaluation of oocytes and embryos used in biomedical application. https://macvetrev.mk/Files/Article/2019/10.2478/macvetrev-2018-0013/macvetrev-2018-0013.pdf 2018-10-15T09:00:00 115 122 10.2478/macvetrev-2018-0013 nucleolus precursor body oocyte embryo transcriptomics epigenetics Michal Benc benc.michal@gmail.com false 1 Department of Zoology and Anthropology, Faculty of Natural Sciences, Constantine the Philosopher University in Nitra, Nabrezie Mladeze 91, 949 74 Nitra, Slovak Republic; Institute of Animal Science, Pratelstvi 815, 104 00 Prague - Uhrineves, Czech Republic LEAD_AUTHOR Lazo Pendovski false 2 Ss. Cyril and Methodius University in Skopje, Lazar Pop - Trajkov 5-7, 1000 Skopje, Republic of Macedonia AUTHOR Matej Murin false 3 Department of Botany and Genetics, Faculty of Natural Sciences, Constantine the Philosopher University in Nitra, Nabrezie Mladeze 91, 949 74 Nitra, Slovak Republic AUTHOR Frantisek Strejcek false 4 Department of Botany and Genetics, Faculty of Natural Sciences, Constantine the Philosopher University in Nitra, Nabrezie Mladeze 91, 949 74 Nitra, Slovak Republic AUTHOR Martin Morovic false 5 Department of Botany and Genetics, Faculty of Natural Sciences, Constantine the Philosopher University in Nitra, Nabrezie Mladeze 91, 949 74 Nitra, Slovak Republic AUTHOR Radek Prochazka false 6 The Czech Academy of Sciences, Institute of Animal Physiology and Genetics, Rumburska 89, 277 21 Libechov, Czech Republic AUTHOR Jozef Laurincik false 7 Department of Zoology and Anthropology, Faculty of Natural Sciences, Constantine the Philosopher University in Nitra, Nabrezie Mladeze 91, 949 74 Nitra, Slovak Republic; The Czech Academy of Sciences, Institute of Animal Physiology and Genetics, Rumburska 89, 277 21 Libechov, Czech Republic AUTHOR Hyttel, P., Fair, T., Callesen, H., Greve, T. 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