ORIGINAL_ARTICLE The aim of this study is to evaluate the success of Brucella spp. isolation in ruminant abortion cases by using different selective media. To this end, 58 samples from ruminant abortion cases were utilized. 4 selective media; namely, Farrell Medium (FM), CITA Medium (CM), Modified Thayer Martin (MTM) and Jones & Morgan (JM) were preferred for isolation. In addition to these, one medium with antibiotics was used to extend the range of the results. Suspensions prepared from organ and fetal stomach contents were inoculated to media plates and incubated at 37Co for 5-8 days in 5-10% CO2 condition. Conventional biotyping method was used to identify Brucella isolates within the level of species and biovar. MTM (67.2%) and Farrell (65.5%) outperformed the other media with regards to isolation rate. However, regarding the inhibition ability against contaminant microrganisms, Farrell (86.2%) and CITA (72%) have the highest and second highest percentages respectively. The media’s inhibition ability was examined in the samples in which Brucella spp. isolation occurred to be able to investigate the correlations between isolation and inhibition. Lower isolation percentage was observed in the samples in which the media displayed the lowest inhibition ability against contaminants. In this context, using two different selective media with high inhibition ability against contaminants may be recommended to enhance the isolation rate. Moreover, the components stimulating the growth of Brucella strains might be added to the media to obtain better results. https://macvetrev.mk/Files/Article/2020/10.2478/macvetrev-2018-0024/macvetrev-2018-0024.pdf 2018-10-15T09:00:00 177 186 10.2478/macvetrev-2018-0024 Biovar Brucella spp. inhibition isolation selective medium Mustafa Sencer Karagul msencerk@hotmail.com false 1 Kartepe Vocational School of Equine Science, Kocaeli University, 41080, Kocaeli, Turkey LEAD_AUTHOR Serkan Ikiz false 2 Department of Microbiology, Faculty of Veterinary Medicine, Istanbul University, 34320, Istanbul, Turkey AUTHOR Godfroid J., Cloeckaert A., Liautard J.P., Kohler S., Fretin D., Walravens K., Garin-Bastuji B., Letesson J.J. (2005). From the discovery of the Malta fever's agent to the discovery of a marine mammal reservoir, brusellosis has continously been a re-emerging zonosis. Vet. Res. 36, 313–326. PMid:15845228 1 10.1051/vetres:2005003 Songer, J.G., K.W. Post. (2012). Brusella cinsi. 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