ORIGINAL_ARTICLE Biochemical effects of oleuropein in streptozotocin induced diabetic male rat Diabetes is a chronic disease characterized by a disorder in the metabolism of proteins, fats, and carbohydrates. The liver as a non-insulin dependent organ plays an important role in the regulation of blood fat and glucose. Most blood glucose lowering drugs that are introduced for treatment have side effects in long-term consumption. Therefore, to control diabetes and its complications, the use of herbal drugs is widely considered nowadays. The present study investigates the biochemical effects of oleuropein in diabetic male rats. In this study, 30 adult male Wistar rats with a weight range of 190±30 gr were equally divided into 3 groups randomly: 1) control group or intact animals, 2) diabetic animals, and 3) treatment group, which received 60 mg/kg oleuropein for 30 days by gastric gavage. Diabetes was induced in diabetic and treatment groups by injection of streptozotocin (60 mg/kg) intraperitoneally. At the end of the treatment, the levels of triglyceride, cholesterol, LDL, HDL, VLDL, blood glucose, HbA1C, and activity of AST and ALT were determined. The results showed that the serum lipid profile and blood glucose increased significantly in the diabetic group compared with the control group (p < 0.05). Also, HbA1C and atherogenic index decreased significantly in the treatment group compared with the diabetic group (p < 0.05). This study showed that oral administration of oleuropein has hypoglycemic effects, which can reduce the serum levels of the lipids profile and the atherogenic index in streptozotocin-induced diabetic male rats. https://macvetrev.mk/Files/Article/2020/10.2478/macvetrev-2018-0021/macvetrev-2018-0021.pdf 2018-10-15T09:00:00 187 194 10.2478/macvetrev-2018-0021 oleuropein serum lipid profile diabetes liver enzymes rat Sina Amirnahavandirahbar false 1 Physiology Department, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran AUTHOR Mohammad Reza Nasirzadeh mr.nasirzadeh@iaut.ac. false 2 Physiology Department, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran LEAD_AUTHOR Risbridger, G., Wang, H., Young, P., Kurti, T., Wang, Y.Z., Lubahn, D. et al. (2001). Evidences that epithelial and mesenchymal estrogen receptor alpha mediates effects of estrogen on prostatic epithelium. Dev Biol. 229, 157–166. PMid:11150243 1 10.1006/dbio.2000.9994 Baynes, J., Thorpe, S. (1999). Role of oxidative stress in diabetic complications:a new perspective on an old paradigm. Diabetes 48, 1 - 9. PMid:9892215 2 10.2337/diabetes.48.1.1 Ullah, A., Khan, A., Khan, I. (2016). Diabetes mellitus and oxidative stress—A concise review. Saudi Pharmaceutical Journal 24(5):547-553. PMid:27752226 PMCid:PMC5059829 3 10.1016/j.jsps.2015.03.013 Meyer, K., Deutscher, J., Anil, M., Berthold, A., Bartsch, G., Kiess, W. (2000). Serum androgen levels in adolescents with type I diabetes:relationship to pubertal stage and metabolic control. J Endocrinol Invest. 23, 362–368. PMid:10908163 4 10.1007/BF03343739 Keith, K.G., Fonseca, V., Tan, M.H., Dalpiaz, A. (2001). Narrative review:Hepatobiliary disease in type 2 diabetes mellitus. Ann. Intern. Med. 141(12):946-956. 5 Kanter, M., Coskun, O., Korkmaz, A., Oter, S. (2004). Effects of Nigella sativa on oxidative stress and βcell Damage in streptozotocin - induced Diabetic rats. Anat Rec A. 279A:685-691. PMid:15224410 6 10.1002/ar.a.20056 Kumar, S., Malhotra, R., Kumar, D. (2010). Antidiabetic and free radical scavenging potential of euphorbia hirta flower extract. Indian J. Pharm. Sci. 72(4):533-537. PMid:21218075 PMCid:PMC3013561 7 10.4103/0250-474X.73921 Al-Jameil, N., Khan, F. A., Arjumand, S., Khan, M. F., Tabassum, H. (2014). Associated liver enzymes with hyperlipidemia profile in type 2 diabetes patients. Int J Clin Exp Pathol. 7(7):4345–4349. PMid:25120819 PMCid:PMC4129054 8 Anup, K.M., Smriti, T., Zabeer, A., Ram, K.S. (2012). Antidiabetic and antihhyperlipidemic effect of Euphorbia hirta in streptozotocin induced diabetic rats. Scholars Research Library 4 (2):703-707. 9 Morrison, J., Dhanasekaran, S., Sheen, R., Frampton, C., Mensah-Brown, E. (2006). The effect of streptozotocin-induced diabetes on the rat seminal vesicle :A possible pathophysiological basis for disorders of ejaculation. Ann. N. Y. Acad. Sci. 1084, 267–279. PMid:17151307 10 10.1196/annals.1372.013 Aljamal, A., Ibrahim, A., Al-Fararjeh, M.A., Alqadi, T. (2012). Effects of rosemary on lipid profile in diabetic rats. Afr J Biochem Res. 6 (7):222-225. 11 Shukla, R., Sharma, S B., Puri, D., Pabhu, K. M., Murthy, P.S. (2006). .Medicinal plants for treatment of diabetes mellitus. Indian J Clin Biochem. 15, 169-177. 12 Olmez, E., Vural, K., Gok, S., Ozturk, Z., Kayalar, H., Ayhan, S., et al. (2015). Olive leaf extractimproves the atherogenic lipid profile in rats fed a high cholesterol diet. Phytother Res. 29(10):1652-1616. PMid:26328503 13 10.1002/ptr.5445 Alirezaei, M., Dezfoulian, O., Kheradmand, A., Neamati, Sh., Khonsari, A., Pirzadeh, A. (2012). Hepatoprotective effects of purified oleuropein from olive leaf extract against ethanol-induced damages in the rat. Iranian Journal of Veterinary Research. 13(3):40. 14 Al Jamal, A., Ibrahim, A. (2011). Effects of olive oil on lipid profiles and blood glucose in type2 diabetic patients. Intentional Journal Diabetes & Metabolism 19, 19-22. 15 Ahmadvand, H., Noori, A., Ghasemi Dehnoo, M., Bagheri, S., Cheraghi, R. (2014). Hypoglycemic, hypolipidemic and antiatherogenic effects of oleuropein in alloxan-induced Type 1 diabetic rats. Asian Pac J Trop Dis. (Suppl 1):S421-S425. 16 10.1016/S2222-1808(14)60481-3 Sangi, S. M. A., Ibrahim, S. M., Fawzy, A. M., Elsamoual, I. A. E., Shaker, A. S. (2015). Antihyperglycemic effect of thymoquinone and oleuropein, on streptozotocin-induced diabetes mellitus in experimental animals. Pharmacogn Mag. 11(2):S251–S257. PMid:26664013 PMCid:PMC4653335 17 10.4103/0973-1296.166017 Nekooeian, A. A., Khalili, A., Khosravi, M. B. (2014). Oleuropein offers cardioprotection in rats with simultaneous type 2 diabetes and renal hypertension. Indian J. Pharmacol. 46(4):398–403. PMid:25097277 PMCid:PMC4118532 18 10.4103/0253-7613.135951 Ahmadvand, H., Bagheri, S., Tamjidi-Poor, A., Cheraghi, M., Azadpour, M., Ezatpour, B., et al. (2016). Biochemical effects of oleuropein in gentamicin-induced nephrotoxicity in rats. ARYA Atheroscler. 12(2):87-93. PMid:27429628 PMCid:PMC4933747 19 Qadir, N. M ., Ali, K. A., Qader, S. W. (2016). Antidiabetic effect of oleuropein from olea europaea leaf against alloxan induced type 1 diabetic in rats. Braz. Arch. Biol. Technol. v.59:e16150116. 20 10.1590/1678-4324-2016150116 Nakhaee, A., Bokaeian, M., Saravani, M., Farhangi, A., Akbarzadeh, A. (2009). Attenuation of oxidative stress in streptozotocin-induced diabetic rats by Eucalyptus Globulus. Indian J Clin Biochem. 24 (4):419-425. PMid:23105871 PMCid:PMC3453050 21 10.1007/s12291-009-0075-1 Ramakrishna, V., Jailkhani, R. (2007). Evaluation of oxidative stress in insulin dependent diabetes mellitus (IDDM) patients. Diagn Pathol. 2, 22. PMid:17603912 PMCid:PMC1936413 22 10.1186/1746-1596-2-22 Gandhi, G. R., Sasikumar, P. (2012). Antidiabetic effect of Merremia emarginated Burm. F. in streptozotocin induced diabetic rats. Asian Pac J Trop Biomed. 2(4):281–286. 23 10.1016/S2221-1691(12)60023-9 Pareek, H., Sharma, S., Khadija, B.S., Jain, K., Jain, GC. (2009). Evaluation of hypoglycemic and anti-hyperglycemic potential of Tridax procumbent (Linn.). BMC Complement Altern Med. 9, 48. PMid:19943967 PMCid:PMC2790435 24 10.1186/1472-6882-9-48 Kondeti, V. K., Badri, K. R., Maddirala, D. R., Thur, S. K. M., Fatima, S. S., Kasetti, R.B., et al. (2010). Effect of Pterocarpus santalinus bark, on blood glucose, serum lipids, and plasma insulin and hepatic carbohydrate metabolic enzymes in streptozotocin induced diabetic rats. Food Chem Toxicol. 48, 1281–1287. PMid:20178824 25 10.1016/j.fct.2010.02.023 Giannini, E.G., Roberto Testa, R., Savarino, V. (2005). Liver enzyme alteration:a guide for clinicians. CMAJ. 172(3):367–379. PMid:15684121 PMCid:PMC545762 26 10.1503/cmaj.1040752 Hassan, H. A., El-Agmy, S. M., Gaur, R. L., Fernando, A., Raj, M. H. G., Outhit, A. (2009). In vivo evidence of hepato and reno-protective effects of garlic oil against sodium nitrite-induced oxidative stress. Int J Biol Sci. 5, 249–255. PMid:19305642 PMCid:PMC2659008 27 10.7150/ijbs.5.249