ORIGINAL_ARTICLE The aim of this study was to carry out the metabolic profile comparison between follicular fluid and serum in normal cows and those affected by ovarian cysts (OC). After slaughtering, blood samples and follicular fluids from normal and cystic animals were collected and assayed using commercial kits to determine the concentrations of metabolites (glucose, total protein, total cholesterol, cortisol, triglycerides, urea, creatinine and insulin) and the liver enzymes activity. Data showed that OC were characterized by low levels of glucose, total protein, cholesterol and cortisol in cystic fluid, while urea concentrations were high compared to normal follicular fluid (P < 0.001). On the other hand, serum assays of cystic animals revealed very low values of insulin and urea, whereas cortisol levels were relatively high in comparison with the serum of normal cows (P < 0.001). Significant correlations between the serum and follicular fluid concentrations of normal cows were found for glucose (r=0.49), total cholesterol (r=0.31), cortisol (r=0.38) and total protein (r=0.63). The highest correlation was found for urea (r=0.86). On contrary, weak correlations were observed between metabolites concentrations in cystic fluid and in serum for normal and cystic cows. In conclusion, OC grow and persist in a metabolic environment, which differs from follicular fluid to blood. These changes may act together and/or separately to ensure the continuous development of OC. To understand a part of the mechanism, the authors propose a deep study about blood-follicle-barrier. https://macvetrev.mk/Files/Article/2020/10.2478/macvetrev-2018-0030/macvetrev-2018-0030.pdf 2019-03-15T09:00:00 51 59 10.2478/macvetrev-2018-0030 ovarian cyst follicular fluid serum metabolic profile Nora Mimoune nora.mimoune@gmail.com false 1 National High School of Veterinary Medicine, Bab-Ezzouar, Algiers, Algeria; Institute of Veterinary Sciences, LBRA, University of Blida 1, PB 270, Soumaa, Blida, Algeria LEAD_AUTHOR Rachid Kaidi false 2 Institute of Veterinary Sciences, LBRA, University of Blida 1, PB 270, Soumaa, Blida, Algeria; School of Veterinary Medicine and Science, University of Nottingham, Nottingham, Leicestershire, United Kingdom AUTHOR Mohammed Hocine Benaissa false 3 Scientific and Technical Research Centre for Arid Areas (CRSTRA), Biophysical Station, Touggourt, Algeria AUTHOR Mohamed Wail Bahouh false 4 National High School of Veterinary Medicine, Bab-Ezzouar, Algiers, Algeria AUTHOR Ratiba Baazizi false 5 National High School of Veterinary Medicine, Bab-Ezzouar, Algiers, Algeria AUTHOR Mohamed Yassine Azzouz false 6 Institute of Veterinary Sciences, LBRA, University of Blida 1, PB 270, Soumaa, Blida, Algeria AUTHOR Ghoneim, I.M., Waheed, M.M., El-Bahr, S.M., Alhaider, A.K., Al-Eknah, M.M. 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