SHORT_COMMUNICATION Chicken astroviruses (CAstV) are enteric viruses of poultry causing gastroenteritis, malabsorption, gout and white chick disease commonly known as runting-stunting syndrome (RSS). It can affect the wide range of poultry birds, especially chicken, turkey and duck worldwide. To our best knowledge there is no published report on presence of antibodies against CAstV in Bangladesh. Therefore, the study aimed to detect the presence of CAstV antibodies in broilers and sonali chickens (a cross-bread) in Bangladesh through a cross-sectional survey. A total of 454 blood samples from 66 flocks of broiler (n=343) and sonali chickens (n=111) of different ages were obtained during 2017 from four districts. The birds were healthy but were not vaccinated against CAstV. The samples were tested for specific antibodies against CAstV Group B by using commercially available ELISA kit. Overall, 16.74% (76/454) samples and 34.84% (23/66) flocks were positive for CAstV antibodies. The seroprevalence of CAstV was significantly (p=0.001) higher in sonali chickens (36.96%) than broiler (10.20%), while it was significantly higher (p=0.001) in birds of Bogura district (36.94%) than the other three districts. Regarding the age groups, seroprevalence was insignificantly (p=0.192) higher in sonali chicken before laying age (45%) than during laying age (27.45%). Regarding the seasons, CAstV infection was prevalent significantly (p=0.001) higher in winter season. Thus, the present study indicated the presence of CAstV in poultry in Bangladesh, so further studies are required to find out the magnitude of the problem in the country. https://macvetrev.mk/Files/Article/2020/10.2478/macvetrev-2020-0016/macvetrev-2020-0016.pdf 2020-03-15T09:00:00 75 80 10.2478/macvetrev-2020-0016 chicken astroviruses poultry antibody seroprevalence survey Md Zulfekar Ali zulfekar@blri.gov.bd false 1 National Reference Laboratory for Avian Influenza, Animal Health Research Division, Bangladesh Livestock Research Institute, Savar, Dhaka 1341, Bangladesh LEAD_AUTHOR Mohammad Moktader Moula false 2 Central Poultry Laboratory, Nourish Poultry and Hatchery Ltd., Dhaka, Bangladesh AUTHOR Zafar Ahmed Bhuiyan false 3 Central Poultry Laboratory, Nourish Poultry and Hatchery Ltd., Dhaka, Bangladesh AUTHOR Muhammad Tariq Javed false 4 Department of Pathology, Faculty of Veterinary Science, University of Agriculture, Faisalabad 38040, Pakistan AUTHOR Matsui, S.M., Greenberg, H.B. (2001). Astroviruses. In: D.M. Knipe, P.M. Howley (Ed.). Fields Virol. 4th ed., Vol. 1. (pp. 875-894). Lippincott Williams and Wilkins, Philadelphia, PA. 1 Kang, K., Icard, A.H., Linnemann, E. (2012). Determination of the full length sequence of a chicken astrovirus suggests a different replication mechanism. Virus Genes 44(1): 45-50. PMid:21879342 2 10.1007/s11262-011-0663-z Méndez,   E.,   Arias,  C.F.   (2013).   Astrovirus. In: D.M. Knipe, P.M. Howley (Ed.). Fields Virology. 6th ed. (pp. 611). Lippincott Williams and Wilkins; Philadelphia, PA, USA. 3 Madeley, C.R., Cosgrove, B.P. (1975).  Letter:  28 nm particles in faeces in infantile gastroenteritis. Lancet 2(7932): 451-452. 4 10.1016/S0140-6736(75)90858-2 Pantin-Jackwood,  M.J.,  Spackman,  E., Woolcock, P.R. (2006). Molecular characterization and typing of chicken and turkey astroviruses circulating in the United States: implications for diagnostics. Avian Dis. 50(3): 397-404. PMid:17039840 5 10.1637/7512-020606R.1 Kang, K.I., Linnemann, E., Icard, A.H., Durairaj, V., Mundt, E., Sellers, H.S. (2018). Chicken astrovirus as an aetiological agent of runting-stunting syndrome in broiler chickens. J. Gen. Virol. 99(4): 512-524. PMid:29458661 6 10.1099/jgv.0.001025 Canelli, E., Cordioli, P., Barbieri, I., Catella, A., Pennelli, D., Ceruti, R., Moreno, A., Lavazza, A. (2012). Astroviruses as causative agents of poultry enteritis: genetic characterization and longitudinal studies on field conditions. Avian Dis. 56(1): 173-182. PMid:22545544 7 10.1637/9831-061311-Reg.1 Bhuiyan, Z.A., Ali, M.Z., Moula, M.M., Bary, M.A., Arefin, N., Giasuddin, M., Khan, Z. U.M. (2019). Seroprevalence of major avian respiratory diseases in broiler and sonali chicken in selected areas of Bangladesh. J. Adv. Vet. Anim. Res. 6(4): 561-566. PMid:31819887 PMCid:PMC6882717 8 10.5455/javar.2019.f383 Bulbule, N.R., Mandakhalikar, K.D., Kapgate, S.S., Deshmukh, V.V., Schat, K.A., Chawak, M.M. (2013). Role of chicken astrovirus as a causative agent of gout in commercial broilers in India. Avian Pathol. 42(5): 464-473. PMid:24015918 9 10.1080/03079457.2013.828194 Smyth, V.J. (2017). A review of the strain diversity and pathogenesis of chicken astrovirus. Viruses 9(2): 29. PMid:28208602 PMCid:PMC5332948 10 10.3390/v9020029 Schultz-Cherry, S., King, D.J., Koci, M.D. (2001). Inactivation of an astrovirus associated with poultry enteritis mortality syndrome. Avian Dis. 45(1): 76-82. PMid:11332502 11 10.2307/1593014 Ali, M.Z. (2018). The seroprevalence study of reticuloendotheliosis virus infection in chicken in Bangladesh. Egypt. J. Vet. Sci. 49(2): 179-186. 12 10.21608/ejvs.2018.5856.1051 Baxendale, W., Mebatsion, T. (2004). The isolation and characterisation of astroviruses from chickens. Avian Pathol. 33(3): 364-370. PMid:15223568 13 10.1080/0307945042000220426 Baksi, S., Rao, N., Chauhan, P., Chauhan,  A. (2018). Sero-prevalence of avian reovirus in broiler breeders in different parts of India. PSM Vet. Res. 3(2): 22-25. 14 Sajewicz-Krukowska, J., Pać, K., Lisowska, A., Pikuła, A., Minta, Z., Króliczewska, B., Domańska-Blicharz, K. (2016). Astrovirus-induced "white chicks" condition-field observation, virus detection and preliminary characterization. Avian Pathol. 45(1): 2-12. PMid:26514927 15 10.1080/03079457.2015.1114173 Nuñez, L.F.N., Santander Parra, S.H., Carranza, C., Astolfi-Ferreira, C.S., Buim, M.R., Piantino Ferreira, A.J. (2016). Detection and molecular characterization of chicken astrovirus associated with chicks that have an unusual condition known as "white chicks" in Brazil. Poult. Sci., 95(6): 1262-1270. PMid:26976903 16 10.3382/ps/pew062 Long, K.E., Ouckama, R.M., Weisz, A., Brash, M.L., Ojkić, D. (2018). White chick syndrome associated with chicken astrovirus in Ontario, Canada. Avian Dis. 62(2): 247-258. PMid:29944402 17 10.1637/11802-012018-Case.1 Bolfa, P., Callanan, J.J., Ketzis, J., Marchi, S., Cheng, T., Huynh, H., Lavinder, T., Boey, K., Hamilton, C., Kelly, P. (2019). Infections and pathology of free- roaming backyard chickens on St. Kitts,  West Indies. J. Vet. Diagn. Invest. 31(3): 343-349. PMid:30973088 18 10.1177/1040638719843638 Sharma, R.N., Dufayet, R., Maufras, T., O'Connell, K., Tiwari, K. (2017). Seroprevalence of antibodies to astrovirus in chickens in Grenada, West Indies. Vet. World 10(6): 636-639. PMid:28717315 PMCid:PMC5499080 19 10.14202/vetworld.2017.636-639 Xue, J., Han, T., Xu, M., Zhao, J., Zhang, G. (2017). The first serological investigation of chicken astrovirus infection in China. Biologicals 47, 22-24. PMid:28347631 20 10.1016/j.biologicals.2017.03.005 Todd,  D.,  Wilkinson,  D.S.,  Jewhurst,  H.L., Wylie, M., Gordon, A.W., Adair, B.M. (2009). A seroprevalence investigation of chicken astrovirus infections. Avian Pathol. 38(4): 301-309. PMid:19937515 21 10.1080/03079450903055421 Biswas, P.K., Uddin, G.M.N., Barua, H., Roy, K., Biswas, D., Ahad, A., Debnath, N.C. (2006). Causes of loss of Sonali chickens on smallholder households in Bangladesh. Prev. Vet. Med. 76(3-4): 185-195. PMid:16774795 22 10.1016/j.prevetmed.2006.05.001