ORIGINAL_ARTICLE New records of the Asian tiger mosquito (aedes albopictus) in North Macedonia Aedes albopictus is an invasive mosquito species spreading throughout Europe and its presence in North Macedonia was recorded in 2016. Following the first detection in September 2018, we conducted a two-week mosquito monitoring by ovitraps in order to determine if there were established populations of Aedes albopictus in Skopje, the capital of North Macedonia. Ninety-four Ae. albopictus eggs (0 to 18 eggs per ovitrap per week) were collected from 7 (14%) ovitraps in 3 (30%) municipalities. Thirty-eight eggs (40.4%) successfully hatched and the adult mosquitoes were identified by morphology and PCR. No other potentially invasive species were identified during the monitoring period. Ae. albopictus distribution is expanding and poses a risk for an Aedes-borne disease transmission in North Macedonia. The available data highlight the need for a regular monitoring for tiger mosquitoes to plan adequate control measures. https://macvetrev.mk/Files/Article/2020/10.2478/macvetrev-2020-0025/macvetrev-2020-0025.pdf 2020-10-15T09:00:00 125 129 10.2478/macvetrev-2020-0025 Aedes albopictus tiger mosquito invasive species insect vector Aleksandar Cvetkovikj acvetkovic@fvm.ukim.edu.mk false 1 Veterinary Institute, Faculty of Veterinary Medicine-Skopje, Ss. Cyril and Methodius University in Skopje, Lazar Pop-Trajkov 5-7, 1000 Skopje, North Macedonia LEAD_AUTHOR Igor Djadjovski false 2 Veterinary Institute, Faculty of Veterinary Medicine-Skopje, Ss. Cyril and Methodius University in Skopje, Lazar Pop-Trajkov 5-7, 1000 Skopje, North Macedonia AUTHOR Kiril Krstevski false 3 Veterinary Institute, Faculty of Veterinary Medicine-Skopje, Ss. Cyril and Methodius University in Skopje, Lazar Pop-Trajkov 5-7, 1000 Skopje, North Macedonia AUTHOR Zagorka Popova false 4 Veterinary Institute, Faculty of Veterinary Medicine-Skopje, Ss. Cyril and Methodius University in Skopje, Lazar Pop-Trajkov 5-7, 1000 Skopje, North Macedonia AUTHOR Ljubica Rashikj false 5 Veterinary Institute, Faculty of Veterinary Medicine-Skopje, Ss. Cyril and Methodius University in Skopje, Lazar Pop-Trajkov 5-7, 1000 Skopje, North Macedonia AUTHOR Kristina Atanasova false 6 Veterinary Institute, Faculty of Veterinary Medicine-Skopje, Ss. Cyril and Methodius University in Skopje, Lazar Pop-Trajkov 5-7, 1000 Skopje, North Macedonia AUTHOR Iskra Cvetkovikj false 7 Veterinary Institute, Faculty of Veterinary Medicine-Skopje, Ss. Cyril and Methodius University in Skopje, Lazar Pop-Trajkov 5-7, 1000 Skopje, North Macedonia AUTHOR Jovana Stefanovska false 8 Veterinary Institute, Faculty of Veterinary Medicine-Skopje, Ss. Cyril and Methodius University in Skopje, Lazar Pop-Trajkov 5-7, 1000 Skopje, North Macedonia AUTHOR Medlock, J. M., Hansford, K. M., Versteirt, V., Cull, B., Kampen, H., Fontenille, D., Hendrickx, G., Zeller, H., Van Bortel, W., Schaffner, F. (2015). An entomological review of invasive mosquitoes in Europe. Bull Entomol Res. 105, 637-663. PMid:25804287 1 10.1017/S0007485315000103 Badieritakis, Ε., Papachristos, D., Latinopoulos, D., Stefopoulou, Α., Kolimenakis, Α., Bithas, K., Patsoula, Ε., Beleri, S., Maselou, D., Balatsos, G., Michaelakis, Α. (2018). Aedes albopictus (Skuse, 1895) (Diptera: Culicidae) in Greece: 13 years of living with the Asian tiger mosquito. Parasitol Res. 117, 453-460. PMid:29275504 2 10.1007/s00436-017-5721-6 Kotsakiozi, P., Richardson, J. B., Pichler, V., Favia, G., Martins, A. J., Urbanelli, S., Armbruster, P. A., Caccone, A. (2017). Population genomics of the Asian tiger mosquito, Aedes albopictus: insights into the recent worldwide invasion. Ecol Evol. 7, 10143-10157. PMid:29238544 PMCid:PMC5723592 3 10.1002/ece3.3514 Becker, N., Schön, S., Klein, A. M., Ferstl, I., Kizgin, A., Tannich, E., Kuhn, C., Pluskota, B., Jöst, A. (2017). First mass development of Aedes albopictus (Diptera: Culicidae)-its surveillance and control in Germany. Parasitol Res. 116, 847-858. PMid:28116530 PMCid:PMC5313584 4 10.1007/s00436-016-5356-z Delatte, H., Desvars, A., Bouétard, A., Bord, S., Gimonneau, G., Vourc'h, G., Fontenille, D. (2010). Blood-feeding behavior of Aedes albopictus, a vector of chikungunya on la réunion. Vector-Borne Zoonotic Dis. 10, 249-258. PMid:19589060 5 10.1089/vbz.2009.0026 Giangaspero, A., Marangi, M., Latrofa, M. S., Martinelli, D., Traversa, D., Otranto, D., Genchi, C. (2013). Evidences of increasing risk of dirofilarioses in southern Italy. Parasitol Res. 112, 1357-1361. PMid:23224639 6 10.1007/s00436-012-3206-1 Sokolovska, N., Kostovska, J., Musa, S., Lazarevska, L., Bajrami, L., Arsenievski, Z. (2017). Our experience in collecting tiger mosquitoes using ovitraps in Strumica, Gevgelija and the border crossing to Greece. J Heal Sci. 5, 345-349. 7 10.17265/2328-7136/2017.06.010 Gunay, F., Picard, M., Robert ,V. (2018). MosKeyTool, an interactive identification key for mosquitoes of Euro-Mediterranean. Version 2.1. In English available at www.medilabsecure.com/moskeytool. Last update: 01/08/2018. 8 Arcà, B., Lombardo, F., Francischetti, I. M. B., Pham, V. M., Mestres-Simon, M., Andersen, J. F., Ribeiro, J. M. C. (2007). An insight into the sialome of the adult female mosquito Aedes albopictus. Insect Biochem Mol Biol. 37, 107-127. PMid:17244540 9 10.1016/j.ibmb.2006.10.007 Carrieri, M., Albieri, A., Angelini, P., Baldacchini, F., Venturelli, C., Zeo, S. M., Bellini, R. (2011). Surveillance of the chikungunya vector Aedes albopictus (Skuse) in Emilia-Romagna (northern Italy): Organizational and technical aspects of a large scale monitoring system. J Vector Ecol. 36, 108-116. PMid:21635648 10 10.1111/j.1948-7134.2011.00147.x Manica, M., Filipponi, F., D'Alessandro, A., Screti, A., Neteler, M., Rosà, R., Solimini, A., della Torre, A., Caputo, B. (2016). Spatial and temporal hot spots of Aedes albopictus abundance inside and outside a South European metropolitan area. PLoS Negl Trop Dis. 10, 1-17. PMid:27333276 PMCid:PMC4917172 11 10.1371/journal.pntd.0004758 Marques, C. C., Marques, G. R., de Brito, M., dos Santos Neto, L. G., Ishibashi, V. C., Gomes, F. A. (1993). Comparative study of the efficiency of larval and ovitraps for the surveillance of dengue and yellow fever vectors. Rev Saude Publica 27, 237-241. PMid:8209154 12 10.1590/S0034-89101993000400002 Kavran, M., Ignjatović Ćupina, A., Zgomba, M., Žunić, A., Bogdanović, S., Srdić, V., Dondur, D., Pudar, D., Marinković, D., Petrić, D. (2019). Invasive mosquito surveillance and first record of Aedes japonicus in Serbia. Proceedings of The IXth EMCA conference. March, 10-14, (p.78), La Rochelle, France. 13 Eritja, R., Palmer, J. R. B., Roiz, D., Sanpera-Calbet, I., Bartumeus, F. (2017). Direct evidence of adult Aedes albopictus dispersal by car. Sci Rep. 7, 1-15. PMid:29070818 PMCid:PMC5656642  14 10.1038/s41598-017-12652-5 Greenberg, H. S., Wilson-Fallon, A. N., Peper, S. T., Haydett, K. M., Presley, S. M. (2019). New records of Aedes aegypti and Aedes albopictus in eight Texas counties, U.S.A. J Vector Ecol. 44, 199-200. PMid:31124239 15 10.1111/jvec.12345 González-Acosta, C., Correa-Morales, F., Canche-Aguilar, I., Silva-Domínguez, R., Salgado-Alonzo, M. C., Muñoz-Urias, R., Salazar-Bueyes, V. M., Moreno-García, M. (2019). First report of Aedes albopictus in Guerrero State, Mexico. J Am Mosq Control Assoc. 35, 285-287. PMid:31922941 16 10.2987/19-6829.1