ORIGINAL_ARTICLE We hypothesized that a single dose of PGF2α belatedly injected on day 8 after GnRH-1 in cows receiving a 7-day Ovsynch-56 protocol (GnRH – 7 days – PGF2α – 56h – GnRH – 16h – timed AI) will increase the proportion of cows with complete luteolysis. At day 35±3 postpartum, 70 lactating Holstein cows from one herd were scored for body condition and pre-synchronized with PGF2α and GnRH (3 days apart) and 7 days later submitted to an Ovsynch-56 protocol for first AI after random assignment to two treatments: (1) OV-7 (n=35) with an injection of PGF2α either on day 7; or (2) OV-8 (n=35) on day 8 after G1, respectively. Blood was collected before the first PGF2α, at day 7 and day 8 in OV-7 and OV-8, respectively, at AI and at 7 days after AI to assess progesterone concentration. Ten cows were classified as acyclic and were excluded from the analysis resulting in 60 cows (OV-8, n=27; OV-7, n=33). In total, more (P=0.01) OV-8 cows and more (P=0.04) primiparous OV-8 cows had complete luteolysis compared with their OV-7 herd mates. In addition, more (P=0.008) OV-8 cows with BCS<2.75 had complete luteolysis compared with their OV-7 herd mates, whereas no difference was observed between treatments among cows with BCS ≥2.75. In conclusion, delaying the application of PGF2α by 1 day reduced the percentage of primiparous cows and cows with poorer BCS having incomplete luteal regression at the time of AI. https://macvetrev.mk/Files/Article/2020/10.2478/macvetrev-2020-0028/macvetrev-2020-0028.pdf 2020-10-15T09:00:00 161 167 10.2478/macvetrev-2020-0028 luteolysis synchronization risk Ovsynch dairy cows BCS Branko Atanasov batanasov@fvm.ukim.edu.mk false 1 Faculty of Veterinary Medicine–Skopje, Ss. Cyril and Methodius University in Skopje, Lazar Pop Trajkov 5-7, 1000 Skopje, R. of N. Macedonia LEAD_AUTHOR Nikola Adamov false 2 Faculty of Veterinary Medicine–Skopje, Ss. Cyril and Methodius University in Skopje, Lazar Pop Trajkov 5-7, 1000 Skopje, R. of N. Macedonia AUTHOR Irena Celeska false 3 Faculty of Veterinary Medicine–Skopje, Ss. Cyril and Methodius University in Skopje, Lazar Pop Trajkov 5-7, 1000 Skopje, R. of N. Macedonia AUTHOR Ksenija Ilievska false 4 Faculty of Veterinary Medicine–Skopje, Ss. Cyril and Methodius University in Skopje, Lazar Pop Trajkov 5-7, 1000 Skopje, R. of N. Macedonia AUTHOR Branko Angjelovski false 5 Faculty of Veterinary Medicine–Skopje, Ss. Cyril and Methodius University in Skopje, Lazar Pop Trajkov 5-7, 1000 Skopje, R. of N. Macedonia AUTHOR Zvonko Trbogazov false 6 Dairy Farm ZSK “Strumica”, Sushica, Strumica, R. of N. Macedonia AUTHOR Filip Davkov false 7 Dairy Farm ZK “Pelagonija”, Bitola, R, of N. Macedonia AUTHOR Toni Dovenski false 8 Faculty of Veterinary Medicine–Skopje, Ss. Cyril and Methodius University in Skopje, Lazar Pop Trajkov 5-7, 1000 Skopje, R. of N. Macedonia AUTHOR Geert Opsomer false 9 Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Ghent, Belgium AUTHOR Jeffrey Stevenson false 9 Department of Animal Sciences and Industry, Kansas State University Manhattan, KS, USA AUTHOR Pursley, J.R., Kosorok, M.R., Wiltbank, M.C. (1997). Reproductive management of lactating dairy cows using synchronization of ovulation. J Dairy Sci. 80(2): 301-306. 1 10.3168/jds.S0022-0302(97)75938-1 Stevenson, J.S. (2016). Physiological predictors of ovulation and pregnancy risk in a fixed-time artificial insemination program, J Dairy Sci. 99(12): 10077-10092. PMid:27720155 2 10.3168/jds.2016-11247 Vasconcelos, J.L.M., Silcox, R.W., Rosa, G.J., Pursley, J.R., Wiltbank, M.C. (1999). Synchronization rate, size of the ovulatory follicle, and pregnancy rate after synchronization of ovulation beginning on different days of the estrous cycle in lactating dairy cows. 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