ORIGINAL_ARTICLE Bovine rotavirus A (BRVA) is a frequent causative agent of diarrhea in neonatal calves. Accurate and rapid diagnosis is crucial to prevent calf mortality from BRVA induced diarrhea. Currently, variety of diagnostic methods are being used to detect BRVA from calves’ feces: antibody-based rapid test and ELISA, and molecular-based RT-PCR and RT-qPCR. The aim of the study was to evaluate the accuracy (sensitivity and specificity) of the rapid test (Immunochromatography), ELISA, and RT-PCR assays, using RT-qPCR as the gold standard, in detection of BRVA in diarrheic calves’ fecal samples. One hundred (n=100) clinically diarrheic fecal samples were tested with four different diagnostic tools. The percent of samples positive by rapid test, ELISA, RT-PCR and RT-qPCR was 10%, 16%, 17%, and 33%, respectively. The agreement between different assays was 75% to 99%. The highest agreement was observed between ELISA and RT-PCR assay (99%). The lowest agreement was recorded (75%) between rapid test and RT-qPCR. The sensitivity of the rapid test, ELISA, and RT-PCR were 30%, 49%, and 52%, respectively when compared to the reference test (RT-qPCR), whereas specificity was 100% for all assays. In conclusion, none of the frequently used diagnostic tests showed a satisfactory level of sensitivity to identify BRVA in calves’ feces. Therefore, the use of a more sensitive rapid test should be used to identify infected calves in field conditions in order to prevent calf mortality from rotaviral diarrhea. https://macvetrev.mk/Files/Article/2021/10.2478/macvetrev-2020-0033/macvetrev-2020-0033.pdf 2021-03-15T09:00:00 37 45 10.2478/macvetrev-2020-0033 calf feces diagnostic assays bovine rotavirus sensitivity and specificity Shama Ranjan Barua samardvm27@gmail.com false 1 Department of Pathology and Parasitology, Chattogram Veterinary and Animal Sciences University, Zakir Hossain, Chattogram 4225, Bangladesh LEAD_AUTHOR Shariful Islam false 2 Department of Pathology and Parasitology, Chattogram Veterinary and Animal Sciences University, Zakir Hossain, Chattogram 4225, Bangladesh AUTHOR А.М.А.М. Zonaed Siddiki false 3 Animal Health Research Division, Bangladesh Livestock Research Institute (BLRI), Dhaka-1341, Bangladesh & EcoHealth Alliance, New York, NY 10001-2023, USA AUTHOR Md Masuduzzaman false 4 Department of Pathology and Parasitology, Chattogram Veterinary and Animal Sciences University, Zakir Hossain, Chattogram 4225, Bangladesh AUTHOR Mohammad Alamgir Hossain false 5 Department of Pathology and Parasitology, Chattogram Veterinary and Animal Sciences University, Zakir Hossain, Chattogram 4225, Bangladesh AUTHOR Sharmin Chowdhury false 6 Department of Pathology and Parasitology, Chattogram Veterinary and Animal Sciences University, Zakir Hossain, Chattogram 4225, Bangladesh AUTHOR Fritzen, J.T., Oliveira, M.V., Lorenzetti, E., Alfieri, A.F., Alfieri, A.A. (2020). Genotype constellation of a rotavirus A field strain with an uncommon G8P [11] genotype combination in a rotavirus vaccinated dairy cattle herd. Arch Virol. 165, 1855-1861. 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