SHORT_COMMUNICATION Severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) is an RNA virus, responsible for the current pandemic outbreak. In total, 200 genomes of the SARS‐CoV‐2 strains from four host organisms have been analyzed. To investigate the presence of the new mutations in the RNA-directed RNA Polymerase (RdRp) of SARS-CoV-2, we analyzed sequences isolated from different hosts, with particular emphasis on human isolates. We performed a search for the new mutations of the RdRp proteins and study how those newly identified mutations could influence RdRp protein stability. Our results revealed 25 mutations in Rhinolophus sinicus, 1 in Mustela lutreola, 6 in Homo sapiens, and none in Mus musculus RdRp proteins of the SARS-CoV-2 isolates. We found that P323L is the most common stabilising radical mutation in human isolates. Also, we described several unique mutations, specific for studied hosts. Therefore, our data suggest that new and emerging variants of the SARS-CoV-2 RdRp have to be considered for the development of effective therapeutic agents and treatments. https://macvetrev.mk/Files/Article/2021/10.2478/macvetrev-2020-0036/macvetrev-2020-0036.pdf 2021-03-15T09:00:00 87 94 10.2478/macvetrev-2020-0036 SARS-CoV-2 mutation RNA-dependent RNA polymerases RdRp Nsp12 Siarhei A. Dabravolski sergedobrowolski@gmail.com false 1 Department of Clinical Diagnostics, Vitebsk State Academy of Veterinary Medicine (UO VGAVM), Dovatora str. 7/11, Vitebsk 21002, Belarus LEAD_AUTHOR Yury K. Kavalionak false 2 Department of Clinical Diagnostics, Vitebsk State Academy of Veterinary Medicine (UO VGAVM), Dovatora str. 7/11, Vitebsk 21002, Belarus AUTHOR Wu, Z., Yang, L., Ren, X., He, G., Zhang, J., Yang, J., Qian, Z., et al. (2016). Deciphering the bat virome catalog to better understand the ecological diversity of bat viruses and the bat origin of emerging infectious diseases. 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