ORIGINAL_ARTICLE Staphylococcus aureus is an important foodborne pathogen due to toxin-related virulence, invasiveness and antibiotic resistance. The ability of S. aureus strains to produce one or more staphylococcal enterotoxins (SEs) in food has been associated with the occurrence of staphylococcal food poisoning (SFP), which is the most common foodborne intoxication worldwide. The study aimed to determine the count of S. aureus strains in samples of raw cow’s milk and various cheeses produced in R. North Macedonia and to detect their ability to produce enterotoxins by passive agglutination SET RPLA (OXOID, UK) and by enzyme-linked fluorescence assay (ELFA) VIDAS SET 2 (Biomerieux, France). A total of 130 S. aureus strains were analyzed. The ability to produce SEs was determined in 17 (13.1%) strains using the SET RPLA detection kit and in 20 (15.4%) strains using the VIDAS SET 2. The study detected enterotoxigenic strains in cheese samples, despite the low count of S. aureus which was below the detection limit according to the Book of rules for microbiological criteria (Off. G. of R.M no 100/2013). Based on these and similar findings, S. aureus must be considered as a possible cause of intoxication, despite the undetected and underreported cases of SFP in the scientific literature. https://macvetrev.mk/Files/Article/2021/10.2478/macvetrev-2021-0014/macvetrev-2021-0014.pdf 2021-03-15T09:00:00 71 77 10.2478/macvetrev-2021-0014 Staphylococcus aureus cheese milk enterotoxins SET RPLA VIDAS SET 2 North Macedonia Marija Ratkova Manovska ratkova.marija@fvm.ukim.edu.mk false 1 Food Institute, Faculty of Veterinary Medicine – Skopje, Ss Cyril and Methodius University in Skopje, Lazar Pop Trajkov 5-7, 1000 Skopje, R. North Macedonia LEAD_AUTHOR Mirko Prodanov false 2 Food Institute, Faculty of Veterinary Medicine – Skopje, Ss Cyril and Methodius University in Skopje, Lazar Pop Trajkov 5-7, 1000 Skopje, R. North Macedonia AUTHOR Ljupcho Angelovski false 3 Food Institute, Faculty of Veterinary Medicine – Skopje, Ss Cyril and Methodius University in Skopje, Lazar Pop Trajkov 5-7, 1000 Skopje, R. North Macedonia AUTHOR Dean Jankuloski false 4 Food Institute, Faculty of Veterinary Medicine – Skopje, Ss Cyril and Methodius University in Skopje, Lazar Pop Trajkov 5-7, 1000 Skopje, R. North Macedonia AUTHOR Pavle Sekulovski false 5 Food Institute, Faculty of Veterinary Medicine – Skopje, Ss Cyril and Methodius University in Skopje, Lazar Pop Trajkov 5-7, 1000 Skopje, R. North Macedonia AUTHOR Normanno, G., Firinu, A., Virgilio, S., Mula, G., Dambrosio, A., Poggiu, A., Decastelli, L., et al. (2005). Coagulase-positive staphylococci and Staphylococcus aureus in food products marketed in Italy. 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