ORIGINAL_ARTICLE Analytical methods based on immunoaffinity column clean-up and quantitative determination with liquid chromatography-fluorescence detection were used to determine aflatoxins and ochratoxin A in liver samples. The validation of the procedures was performed. The linearity of the methods was checked, and a good coefficient of correlation was found for all aflatoxins and OTA as well. The LOD and LOQ were acceptable: 0.003 μg/kg and 0.009 μg/kg for AFB1; 0.001 μg/kg and 0.005 μg/kg for AFB2; 0.006 μg/kg and 0.020 μg/kg for AFG1; 0.007 μg/kg and 0.022 μg/kg for AFG2; 0.08 μg/kg and 0.27 μg/kg for OTA. The results for the repeatability estimated by the relative standard deviation (RSDr) were satisfactory and the obtained values were in the acceptable range (1.97–14.41% for all aflatoxins and 3.76-8.31% for OTA) at three proposed concentration levels. RSDR values showed acceptable correlation between two analysts for all four aflatoxins and OTA. The RSDR values were as followed: 2.37% and 5.60% for AFB1, 6.71% and 8.78% for AFB2, 4.40% and 7.00% for AFG1 and 10.30% and 13.91% for AFG2 (for the first and second analyst, respectively). The RSDR values for OTA were 4.91% and 3.15% (1 μg/kg); 3.76% and 4.12% (5 μg/kg) and 8.31% and 8.21% (10 μg/kg). The mean recovery for total aflatoxins and OTA were 78.10% and 93.34%, respectively. All validation parameters were in accordance to European legislation. They indicate that the proposed analytical procedures are suitable and they could be methods of choice for the determination of aflatoxins and OTA in liver samples. https://macvetrev.mk 2021-10-15T09:00:00 119 127 10.2478/macvetrev-2021-0017 aflatoxins ochratoxin A HPLC-FD liver samples validation Biljana Stojanovska-Dimzoska bsdimzoska@fvm.ukim.edu.mk false 1 Food Institute, Faculty of Veterinary Medicine Skopje, Lazar Pop Trajkov 5-7, 1000 Skopje, R. North Macedonia LEAD_AUTHOR Elizabeta Dimitrieska-Stojkovic false 2 Food Institute, Faculty of Veterinary Medicine Skopje, Lazar Pop Trajkov 5-7, 1000 Skopje, R. North Macedonia AUTHOR Zehra Hajrulai-Musliu false 3 Food Institute, Faculty of Veterinary Medicine Skopje, Lazar Pop Trajkov 5-7, 1000 Skopje, R. North Macedonia AUTHOR Risto Uzunov false 4 Food Institute, Faculty of Veterinary Medicine Skopje, Lazar Pop Trajkov 5-7, 1000 Skopje, R. North Macedonia AUTHOR Aleksandra Angeleska false 5 Food Institute, Faculty of Veterinary Medicine Skopje, Lazar Pop Trajkov 5-7, 1000 Skopje, R. North Macedonia AUTHOR Dean Jankuloski false 6 Food Institute, Faculty of Veterinary Medicine Skopje, Lazar Pop Trajkov 5-7, 1000 Skopje, R. North Macedonia AUTHOR Katerina Blagoevska false 7 Food Institute, Faculty of Veterinary Medicine Skopje, Lazar Pop Trajkov 5-7, 1000 Skopje, R. North Macedonia AUTHOR European Food Safety Authority (EFSA). (2006). 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