ORIGINAL_ARTICLE Due to the actuality of spongiform encephalopathies and their proven spreading by means of animal feed containing meat and bone meal, the description and measurement of osteocytic lacunae contributes to more easily distinguish bone fragments in meat and bone meal. Transmissible spongiform encephalopathies (TSEs) have attracted a lot of attention, especially after 1986, when the first case of BSE (bovine spongiform encephalopathy) was detected. Since the outbreak of spongiform encephalopathy (BSE), the use of animal protein including bone meal as an ingredient in animal feed has been controlled by several regulations including Regulation (EC) 999/2001, Regulation (EC) 1774/2002, and Regulation (EC) 1234/2003. The classical microscopic method is the only official method for detecting animal protein in animal feed in the European Union (Commission Regulation (EC) 152/2009). By applying the microscopic method to the animal feed samples, we performed detection in order to determine the presence of animal proteins that originate from mammals and fish. The microscopic analysis of all 421 samples, of which 115 were raw materials for the production of animal feed, 230 were concentrates for ruminant nutrition and 76 were concentrates for non-ruminant nutrition (32 concentrates for laying hens and 44 concentrates for pigs), did not provide positive results, that is, no remains of animal tissues of mammalian origin were found in any specimen. Whereas in 10 out of 32 (31.25%) concentrates intended for non-ruminant nutrition (laying hens), pieces of fish tissue were found. In these samples, we usually detected the presence of fish bones, gills and scales. https://macvetrev.mk/Files/Article/2021/10.2478/macvetrev-2021-0021/macvetrev-2021-0021.pdf 2021-10-15T09:00:00 169 178 10.2478/macvetrev-2021-0021 animal proteins in animal feed microscopy decision support system ARIES Radmila Crceva Nikolovska rnikolovska@fvm.ukim.edu.mk false 1 Faculty of Veterinary Medicine, University “Ss. Cyril and Methodius”, Lazar Pop Trajkov 5-7, 1000 Skopje, Republic of North Macedonia LEAD_AUTHOR Aleksandra Angeleska false 2 Faculty of Veterinary Medicine, University “Ss. Cyril and Methodius”, Lazar Pop Trajkov 5-7, 1000 Skopje, Republic of North Macedonia AUTHOR Srecko Gjorgjievski false 3 Institute of Animal Biotechnology, Faculty of Agricultural Sciences and Food, University “Ss. Cyril and Methodius”, 1000 Skopje, Republic of North Macedonia AUTHOR Katerina Blagoevska false 4 Faculty of Veterinary Medicine, University “Ss. Cyril and Methodius”, Lazar Pop Trajkov 5-7, 1000 Skopje, Republic of North Macedonia AUTHOR Riste Uzunov false 5 Faculty of Veterinary Medicine, University “Ss. Cyril and Methodius”, Lazar Pop Trajkov 5-7, 1000 Skopje, Republic of North Macedonia AUTHOR Vasilka Poposka Trenevska false 6 Faculty of Veterinary Medicine, University “Ss. Cyril and Methodius”, Lazar Pop Trajkov 5-7, 1000 Skopje, Republic of North Macedonia AUTHOR Hendriks, W.H., Butts, C.A., Thomas, V.D., James, K.A.C., Morel, P.C.A., Verstegen, M.W.A. (2002). Nutritional quality and variation of meat and bone meal. Asian-Aust. J. Anim. Sci. 15(10): 1507-1516. 1 10.5713/ajas.2002.1507 Rodehutscord, M., Abel, H.J., Friedt, W, Wenk, C., Flachowsky, G.,Ahlgrimm, H.J., Johnke, B., et al. (2002). Consequences of the ban of by-products from terrestrial animals in livestock feeding in Germany and the European Union: alternatives, nutrient and energy cycles, plant production, and economic aspects. Arch Tierernahr. 56, 67-91. 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