ORIGINAL_ARTICLE The study was planned to evaluate the use of laparoscopy for the diagnosis and retrieval of abdominal cysts in sheep and goats. The abdominal cysts were located in 10 of 135 adult, healthy small ruminants by using ultrasonography (USG). Percutaneous Puncture-Aspiration-Injection-Reaspiration (PAIR) technique was used for six animals. Thirty animals, including four diagnosed with abdominal cysts by USG, were subsequently subjected to laparoscopy under diazepam sedation (0.1 mg/kg, IV) and lumbosacral epidural regional anesthesia using 2% lignocaine hydrochloride (1.0 ml/5 kg). The animals were restrained in dorsal recumbency and two-port paramedian laparoscopy was performed. Abdominal cysts were located in seven animals (one cyst/animal). The cysts were grasped carefully, lifted close to the abdominal wall, and evacuated percutaneously under laparoscopic vision. The collapsed cysts were then retrieved. In two animals enlarged ports were sutured, treated with an antibiotic and an analgesic, resulting in uneventful healing. Five cysts were nonparasitic, two were parasitic, one extracted from a sheep, and one from a goat. In conclusion, laparoscopy has higher diagnostic accuracy in detecting unattached abdominal cysts in sheep and goats compared to USG. It is also a reliable, minimally invasive, and safe procedure for cyst retrieval. However, a larger-scale study is necessary for ascertaining long-term complications and the recurrence rate. https://macvetrev.mk/Files/Article/2021/10.2478/macvetrev-2021-0022/macvetrev-2021-0022.pdf 2021-10-15T09:00:00 225 233 10.2478/macvetrev-2021-0022 abdomen cyst goat laparoscopy sheep ultrasonography Nida Handoo false 1 Division of Veterinary Surgery and Radiology, Faculty of Veterinary Sciences and AH, Shere Kashmir University of Agricultural Sciences and Technology of Kashmir, Kashmir, India AUTHOR Mujeeb Fazili fazili_mr@yahoo.co.in false 2 Division of Veterinary Clinical Complex, Faculty of Veterinary Sciences SKUAST-Kashmir, Srinagar, Kashmir, India LEAD_AUTHOR Riyaz Ahmad Shah false 3 Division of Animal Biotechnology, Faculty of Veterinary Sciences and AH, Shere Kashmir University of Agricultural Sciences and Technology of Kashmir, Kashmir, India AUTHOR Hakim Athar false 4 Division of Veterinary Clinical Complex, Faculty of Veterinary Sciences SKUAST-Kashmir, Srinagar, Kashmir, India AUTHOR Idrees Allai false 5 Division of Veterinary Parasitology, Faculty of Veterinary Sciences and AH, Shere Kashmir University of Agricultural Sciences and Technology of Kashmir, Kashmir, India AUTHOR Bisma Kashani false 6 Division of Veterinary Pathology, Faculty of Veterinary Sciences and AH, Shere Kashmir University of Agricultural Sciences and Technology of Kashmir, Kashmir, India AUTHOR Masood Saleem Mir false 7 Division of Veterinary Pathology, Faculty of Veterinary Sciences and AH, Shere Kashmir University of Agricultural Sciences and Technology of Kashmir, Kashmir, India AUTHOR Mathai, R., Bhatt, R.H., Jhala, S., Kelawala, N.H., Patil, D.B., Parikh, P.V. (2008). Current diagnostic techniques in veterinary surgery. Vet World. 1(3): 90-91. 1 Bayne, J.E., Edmondson, M.A. (2021). Diseases of the gastrointestinal system. In: D.G. Pugh (Ed.), Sheep, goat and cervid medicine, (pp. 63-96). 3rd edition, Elsevier. PMCid:PMC7169351 2 10.1016/B978-0-323-62463-3.00014-1 Del Poggio, P., Buonocore, M. (2008). Cystic tumors of the liver: a practical approach. World J Gastroenterol. 14(23): 3616-3620. PMid:18595127 PMCid:PMC2719223 3 10.3748/wjg.14.3616 Dore, F., Varcasia, A., Pipia, P., Sanna, G., Parpaglia, M.L.P., Corda, A., Romic, T., Antonio, S. (2014). Ultrasound as a monitoring tool for cystic echinococcosis in sheep. Vet Parasitol. 203(1-2): 59-64. PMid:24704343 4 10.1016/j.vetpar.2014.03.016 Cariati, A., Tavian, M., Lo Casto, R.M., Morelli, N., Piromalli, E., Cariati, E. (2014). Role of surgery and minimally invasive approach in multi-organ localization of cystic echinococcosis. Arch Clin Exp Surg. 3(2): 102-106. 5 10.5455/aces.20130306052457 Bickel, A., Loberant, N., Singer-Jordan, J., Goldfeld, M., Daud, G., Eitan, A. (2001). The laparoscopic approach to abdominal hydatid cysts: a prospective nonselective study using the isolated hyperbaric technique. Arch Surg. 136, 789-795. PMid:11448392 6 10.1001/archsurg.136.7.789 Wieczorek, J., Kosenyuk, Y., Kareta, W., Cegła, M. (2007). Oocyte recovery rate with application of laparoscopic ovum pick-up in pigs. Ann Anim Sci. 7(1): 89-97. 7 Franz, S., Dadak, A.M., Schoffmann, G., Khol, J.L., Baumgartner, W., Dupre, G. (2009). Laparoscopicassisted cystotomy: an experimental study in male sheep. Vet Med (Praha). 54(8): 367-373. 8 10.17221/106/2009-VETMED Handoo, N., Fazili, M.R., Gayas, M.A., Shah, R.A., Malik, A., Athar, H., Khan, H.M. (2020). Successful laparoscope-assisted orchiectomy in three cryptorchid sheep. Vet Anim Sci. 9, 100112. PMid:32734113 PMCid:PMC7386778 9 10.1016/j.vas.2020.100112 Handoo, N., Fazili, M.R., Gayas, M.A., Athar, H., Raja, T. (2021). Percutaneous aspiration-injectionreaspiration (PAIR) for the treatment of abdominal cysts: Initial report in sheep and goat. Acta Vet Hung. 393-398. PMid:33459611 10 10.1556/004.2020.00059 Luna, L.G. (1968). Manual of histologic staining methods of the armed forces institute of pathology. 3rd ed. New York: Blakiston Division, McGraw-Hill. 11 Fazili, M.R. (2020). Laparoscopic surgery in ruminants. In: J. Singh, S.S. Singh, R.P.S. Tyagi (Eds.), Ruminant surgery (pp. 279-291). 2nd ed. New Delhi: CBS Publishers and Distributors Pvt Ltd. 12 Sage, A.M., Wachira, T.M., Zeyhle, E.E., Weber, E.P., Njoroge, E., Smith, G. (1998). Evaluation of diagnostic ultrasound as a mass screening technique for the detection of hydatid cysts in the liver and lung of sheep and goats. Int J Parasitol. 28(2): 349-353. 13 10.1016/S0020-7519(97)00187-2 Mohammed, N.M.O. (2009). Ultrasonographic and laparoscopic studies on the abdomen in sheep and goats. Faculty of Veterinary Medicine, Cairo University. [Dissertation]. p. 225. 14 Khuroo, M.S. (2002). Hydatid disease: current status and recent advances. Annals of Saudi Medicine. 22(1-2): 56-64. PMid:17259768 15 10.5144/0256-4947.2002.56 Fazili, M.R., Shah, R.A., Khan, F.A., Khajuria, A., Yaqoob, S.H., Naykoo, N.A., Ganai, N.A. (2015). Normal laparoscopic anatomy of the caprine pelvic cavity. Vet Sci Dev. 5, 6012. 16 10.4081/vsd.2015.6012 Khajuria, A., Fazili, M.R., Shah, R.A., Bhat, M.H., Khan, F., Syed, H.Y., Naykoo, N.A., Ganai, N.A. (2014). Comparison between lignocaine hydrochloride and ropivacaine hydrochloride as lumbosacral epidural anaesthetic agents in goats undergoing laparoscopy assisted embryo transfer. Mac Vet Rev. 37(2): 141-149. 17 10.14432/j.macvetrev.2014.06.017 Khajuria, A., Fazili, M.R., Shah, R.A., Khan, F.A., Bhat, M.H., Syed, H.Y., Naykoo, N.A., Ganai, N.A. (2014). Comparison of two doses of ropivacaine hydrochloride for lumbosacral epidural anaesthesia in goats undergoing laparoscopy assisted embryo transfer. Int Sch Res Notices. 2014, 937018. PMid:27382609 PMCid:PMC4897202 18 10.1155/2014/937018 Leil, A.Z., Shaker, M.H., El-Badry, D.A.M. (2014). Studies on laparoscopic intrauterine insemination of Barki ewes (using different insemination doses) as compared with cervical insemination. Assiut Vet Med J. 60(142): 172-178. 19 10.21608/avmj.2014.171004 Harrison, R.M., Wildt, D.T. (1980). Animal laparoscopy. Baltimore, Maryland: Williams and Wilkins Co. 20 Kassem, M.M., Abdel-Wahed, R.E., El-Gendy, S.A.A., El-Kammar, M.H. (2008). Laparoscopic anatomy of caprine abdomen and laparoscopic liver biopsy. Kafrelsheikh Vet Med J. 6(1): 94-113. 21 10.21608/kvmj.2008.115901 Boure, L. (2005). General principles of laparoscopy. Vet Clin North Am Food Anim Pract. 21(1): 227-249. PMid:15718094 22 10.1016/j.cvfa.2004.12.002 Bensghir, M., Fjouji, S., Bouhabba, N., Ahtil, R., Traore, A., Azendour, H., Kamili, N.D. (2012). Anaphylactic shock during hydatid cyst surgery. Saudi J Anaesth. 6(2): 161-164. PMid:22754444 PMCid:PMC3385260 23 10.4103/1658-354X.97031 Soulsby, E.J.L. (1976). Helminths, arthropods and protozoa of domestic animals. 6th ed. Of Monnig's veterinary helminthology and entomology (pp. 126-131). The English language book society and baillere. London: Tindall & Cassell Ltd. 24 Admasu, Y., Tadesse, T., Reta, Z. (2019). Study on prevalence of small ruminant cysticercus tenuicollis and its monetary loss at Bishoftu Elfora export abattoir, Oromia, Ethiopia. Dairy Vet Sci J. 9(2): 555759. 25 10.19080/JDVS.2019.09.555759 Khaled, K., Teber, G., Faten Bouaicha, F., Amairia, S., Rekik, M., Gharbi, M. (2019). Infestation of small ruminants by the metacestode stage of Taenia hydatigena in slaughterhouse, North East Tunisia. Vet Med Sci. 6(2): 204 - 208. PMid:31782917 PMCid:PMC7196678 26 10.1002/vms3.222 Ramani, M., Husain, K.W., Radhika Krishna, O., Reddy, R., Reddy, P.S., Kumar, D.P., Sunitha, G. (2013). Histopathological review of mesenteric and omental cysts in children. J Evol Med Dent Sci. 2(26): 4701 - 4709. 27 10.14260/jemds/901