ORIGINAL_ARTICLE The development of an antimicrobial stewardship plan (AMSP) for live bird sellers (LBS) requires an understanding of the current biosecurity status, antimicrobial use (AMU) and the practices involved in live bird selling (e.g., drivers, sellers, etc.) which is a direct source of poultry meat for human consumption. Seven Live Bird Markets (LBMs) within Abeokuta, Ogun State were surveyed using a semi-structured questionnaire. Data on LBMs characteristics, LBS demographics, biosecurity, and AMU practices, awareness on Antimicrobial Resistance (AMR), as well as preferred channels of information on antimicrobial stewardship were gathered. A total of 40 consenting LBS with 82.5% female and 17.5% male participants were included in the study. The participants’ mean age was 45.3 years (SD±11.9, range: 23-70 years). Laying hens, broilers, and cockerels were the main poultry types sold by LBS. Antimicrobials (AMs) were used for growth promotion (57.5%), therapeutic (40.0%), and prophylactic (2.5%) purposes. Tetracycline, metronidazole, and chloramphenicol were the most frequently used AMs. The majority of the participants (90.0%) have treated birds based on their empirical experience, with little or no inputs from veterinarians. Biosecurity and AMU practices were generally low (54.0% and 34.0%, respectively). The contact with veterinarians was associated with satisfactory biosecurity practices (p=0.049). No significant factors were found to be linked with AMU. This study has provided recent evidence-based data on practices in poultry management among LBS in Abeokuta, Ogun state. The findings would be useful for policy decisions and the development of AMSP on prudent AMU among LBS. https://macvetrev.mk/Files/Article/2021/10.2478/macvetrev-2021-0024/macvetrev-2021-0024.pdf 2021-10-15T09:00:00 i ixvi 10.2478/macvetrev-2021-0024 antimicrobial use biosecurity live bird markets live bird sellers risk factors Abeokuta Oluwawemimo Adebowale adebowaleoluwawemimo1@gmail.com false 1 Department of Veterinary Public Health and Preventive Medicine, Federal University of Agriculture, Abeokuta, Nigeria LEAD_AUTHOR Motunrayo Makanjuola false 2 Department of Veterinary Public Health and Preventive Medicine, Federal University of Agriculture, Abeokuta, Nigeria AUTHOR Noah Bankole false 3 Department of Veterinary Microbiology, Federal University of Agriculture, Abeokuta, Nigeria AUTHOR Olanike Adeyemo false 4 Department of Veterinary Public Health and Preventive Medicine, Federal University of Agriculture, Abeokuta, Nigeria AUTHOR Ayomikun Awoseyi false 5 Department of Mathematics and Computer Sciences, First Technical University, Ibadan, Nigeria AUTHOR Olajoju Jokotola Awoyomi false 6 Department of Veterinary Public Health and Preventive Medicine, Federal University of Agriculture, Abeokuta, Nigeria AUTHOR Fagbamila, I.O., Barco, L., Mancin, M., Kwaga, J., Ngulukun, S.S., Zavagnin, P., Lettini, A.A. 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