SHORT_COMMUNICATION The development and implementation of new and safe means of treating subclinical mastitis (SM) in the production process remains a pressing problem. Our study aimed to investigate the therapeutic effect of the Bacillus megaterium NCH 55 strain on cow SM. All animals with signs of SM were fed with a basic diet with probiotic’s addition of 15 g/per day. The experiment was conveyed in 30 consecutive days. Milk and blood were collected on the 7th, 15th, and 30th day of the experiment. Daily milk yield was increased by 32.2% and somatic cell count was decreased from 290.00±32.12 to 96.80±39.03 ths/cm3. A decrease in inflammation was indicated by a decrease in POM370 (by 2.2 times) and POM430 (by 2.8 times). No significant changes in the blood serum indices of cows were observed. A probiotic’s positive effect has been established on the treatment of SM. The probiotic B. megaterium NСH 55 can be considered as an alternative to antibiotics and other chemotherapeutic drugs for treating SM in cows. https://macvetrev.mk 2022-10-15 209 2014 10.2478/macvetrev-2022-0023 cow mastitis milk probiotics Bacillus megaterium Oksana Shkromada false 1 Department of Obstetrics and Surgery, Faculty of Veterinary Medicine, Sumy National Agrarian University, Sumy, Ukraine AUTHOR Alina Pikhtirova a.pikhtirova@med.sumdu.edu.ua false 2 Department of Public Health, Medical Institute, Sumy State University, Sumy, Ukraine LEAD_AUTHOR Ewa Pecka-Kiełb false 3 Department of Biostructure and Animal Physiology, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland AUTHOR Oleksandr Skliar false 4 Department of Therapy, Pharmacology, Clinical Diagnostics and Chemistry, Faculty of Veterinary Medicine, Sumy National Agrarian University, Sumy, Ukraine AUTHOR Yurii Musiienko false 5 Department of Obstetrics and Surgery, Faculty of Veterinary Medicine, Sumy National Agrarian University, Sumy, Ukraine AUTHOR Ruegg, P.L. 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