ORIGINAL_ARTICLE Synthetic detergents which have a major role in environmental pollution accumulate over time and reach levels that harm nature. The surfactants which are abundantly used as cleaning components are discharged into the Van Lake with the sewage water. These chemicals accumulating in the lake may reach a level that could affect the only fish species of the lake, the Van fish. This study aimed to determine the antioxidant levels of Van fish hepatocyte cell culture medium treated with sodium lauryl sulphate (SLS) and to assess the DNA damage. The effect of SLS was assessed by its dose (1x10-5, 1x10-6, 1x10-7 M) and treatment time (24 h, 48 h). Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), malondialdehyde (MDA), and DNA damage (8-OHdG) were determined in the SLS hepatocyte culture. SOD and GSH-Px were higher on 24 h and 48 h compared to the control group. A significant increase was observed in CAT level in the first 24 h, especially in 1x10-6 and 1x10-5 M concentration. At 48 h, it was observed that the CAT level decreased significantly as the concentration increased. It was determined that MDA and 8-OHdG levels increased depending on concentration and time. In conclusion, different concentrations of SLS affected antioxidant levels in the primary hepatocyte culture of Van Fish and were found to cause an increase in the levels of MDA and 8-OHdG. https://macvetrev.mk/Files/Article/2022/10.2478/macvetrev-2022-0027/macvetrev-2022-0027.pdf 2022-10-15 169 175 10.2478/macvetrev-2022-0027 SLS fish DNA damage antioxidants MDA Aslı Ç. Yeltekin aslicyeltekin@gmail.com false 1 Department of Chemistry, Faculty of Science, University of Van Yuzuncu Yil, 65080, Turkey LEAD_AUTHOR Ahmet R. Oğuz false 2 Department of Biology, Faculty of Science, University of Van Yuzuncu Yil, 65080, Turkey AUTHOR Holland, P.M., Rubingh, D.N. (1992). 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