ORIGINAL_SCIENTIFIC_ARTICLE The incidence of clinical mastitis (CM) in small and large-scale dairy cow farms can be highly variable and can be affected by age, parity, post-calving status, and atmospheric conditions. The current study aimed to assess the CM-incidence and its association with the post-calving days, number of lactations, season, and number of affected udder quarters in dairy cows in small-scale dairy farms. The study was conducted within one calendar year in 177 small-scale farms with 864 dairy cows. Clinically confirmed CM cows (n=72) were sampled from each udder quarter and processed for bacteriology examination. The CM-positive samples were grouped according to the season (spring, summer, autumn, and winter), the number of days after calving (<90, 90-180, and >180), the number of lactations (1-st, 2-5-th, and >6-th), and the number of affected udder quarters (one, two, three, and four). The CM-positive samples (n=72, 8.3%) were confirmed on bacteriology examination in significantly lower count (n=56, 6.5%). The 2-5-th lactations cows (n=35, 68.6%) were significantly more compared to the first lactation (n=6, 11.8%), and >6-th lactation cows (n=10, 19.6%). CM cows with infection of one udder quarter (n=40, 78.4%) were significantly more than the cows with two (n=6, 11.8%), and four infected quarters (n=5, 9.8%).The CM-incidence in small-scale dairy cow farms in North Macedonia was 8.3% and 6.5% by clinical and bacteriology examination, respectively. The highest CM susceptibility was observed in the cows between the second and fifth lactations.One udder quarter was most frequently affected in CM-positive cases. https://macvetrev.mk/LoadArticlePdf/365 2023-3-15 35 41 10.2478/macvetrev-2023-0010 mastitis cows lactation calving parity Aleksandar Janevski aleksandar_janevski@yahoo.com false 1 Department of Farm Animals Internal Medicine, Faculty of Veterinary Medicine-Skopje,Ss. Cyril and Methodius University, Lazar Pop-Trajkov 5-7, 1000 Skopje, N. Macedonia LEAD_AUTHOR Martin Nikolovski false 2 Department of Functional Morphology, Faculty of Veterinary Medicine-Skopje,Ss. Cyril and Methodius University, Lazar Pop-Trajkov 5-7, 1000 Skopje, N. Macedonia AUTHOR Iskra Cvetkovikj false 3 Department of Microbiology and Immunology, Faculty of Veterinary Medicine-Skopje,Ss. Cyril and Methodius University, Lazar Pop-Trajkov 5-7, 1000 Skopje, N. Macedonia AUTHOR Silvana Andreevska false 4 Veterinary Clinic “Pro-Infovet”, 1 bb, Petrovec, N. Macedonia AUTHOR Angjelche Todorovski false 5 4Veterinary Clinic “Pro-Infovet”, 1 bb, Petrovec, N. Macedonia AUTHOR Dine Mitrov false 6 Department of Farm Animals Internal Medicine, Faculty of Veterinary Medicine-Skopje,Ss. Cyril and Methodius University, Lazar Pop-Trajkov 5-7, 1000 Skopje, N. Macedonia AUTHOR De Vliegher, S., Fox, L.K., Piepers, S., McDougall, S.,Barkema, H.W. (2012). Invited review: Mastitis in dairy heifers; nature of the disease, potential impact, prevention, and control. J Dairy Sci. 95(3): 1025-1040. 1 https://doi.org/10.3168/jds.2010-4074 Nakov, D., Trajcev, M. (2012). Udder quarter risk factors associated with prevalence of bovine clinical mastitis. Mac Vet Rev. 35(2): 55-64. 2 Tomanić, D., Božin, B., Čabarkapa, I., Kladar, N.,Radinović, M., Maletić, M., Kovačević, Z. (2022).Chemical composition, antioxidant and antibacterial activity of two different essential oils against mastitis associated pathogens. 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