ORIGINAL_SCIENTIFIC_ARTICLE The study aimed to investigate the Staphylococcus species from dogs with chronic otitis externa in Istanbul and to determine their antibiotic susceptibility patterns. Ear swab samples were collected from 100 dogs suspected of otitis externa admitted at the clinics of Istanbul University - Cerrahpaşa, Faculty of Veterinary Medicine. The dogs were of different age, gender, and breed. The bacterial isolation was performed by conventional methods. BD Phoenix Automated Microbiology System was used to confirm bacterial identification by conventional methods and to test antimicrobial susceptibility. Staphylococcus spp. were isolated from 36% of the samples collected from the dogs. S. pseudintermedius, S. aureus, S. epidermidis, S. hyicus and S. chromogenes were identified in 41.6%, 22.2%, 11.1%, 5.5%, and 5.5%. In Staphylococcus spp. isolates, enrofloxacin, penicillin, and ampicillin-sulbactam resistance was 8.3%, marbofloxacin resistance was 11.1%, doxycycline resistance was 16.6%, amoxicillin-clavulanic acid, erythromycin, and gentamicin resistance was 19.4%, tetracycline, clindamycin, and sulphonamide resistance was 25%. Methicillin resistance was not observed in any of the isolates. However, multiple drug resistance (MDR) was detected in 11 (30.5%) of 36 isolates. In conclusion, the early detection and antimicrobial sensitivity testing of Staphylococcus spp in dog otitis externa cases that do not respond to empiric therapy could be beneficial for appropriate antibiotic selection and treatment thus preventing MDR https://macvetrev.mk/LoadArticlePdf/360 2023-3-15 43 50 10.2478/macvetrev-2023-0012 antimicrobial resistance Staphylococcus canine otitis externa Mohammad Hassan mhemedhassan88@gmail.com false 1 Department of Microbiology, Institute of Graduate Studies, Istanbul Unıversity-Cerrahpasa, Avcilar, 34315 Istanbul, Türkiye AUTHOR Ayse Ilgin Kekeç ilginkekec@iuc.edu.tr false 2 Department of Microbiology, Faculty of Veterinary Medicine, Istanbul University-Cerrahpaşa, Büyükçekmece, 34500 Istanbul, Türkiye LEAD_AUTHOR Barış Halaç baris.halac@iuc.edu.tr false 3 Department of Microbiology, Faculty of Veterinary Medicine, Istanbul University-Cerrahpaşa, Büyükçekmece, 34500 Istanbul, Türkiye AUTHOR Beren Başaran Kahraman beren@iuc.edu.tr false 4 Department of Microbiology, Faculty of Veterinary Medicine, Istanbul University-Cerrahpaşa, Büyükçekmece, 34500 Istanbul, Türkiye AUTHOR Fernández, G., Barboza, G., Villalobos, A., Parra, O., Finol, G., Ramirez, R. 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