ORIGINAL_SCIENTIFIC_ARTICLE This study aimed to investigate the effects of dietary supplementation with modified-hen-egg-yolk on plasma lipids and lipoprotein profiles in rats. During the four-week-experiment, 64 Wistar rats were divided into four groups of 16 (eight of both sexes), and fed commercial rat food (group C); food containing 70% commercial rat mixture and 30% freshly cooked egg yolk originating from laying hen eggs fed with 3% fish oil (group F); 3% palm olein (group P), or 3% lard (group L). The cooked egg yolk in the rat diet affected the concentrations of plasma total and LDL-cholesterol in males of the P and L groups. Cholesterol and total fat in the diet did not have a hypercholesterolemic effect on their own, but when in combination with fatty acid composition, they could contribute to an increase in plasma total and LDL cholesterol concentrations in rats. HDL-cholesterol was the most resilient plasma lipoprotein of rats to dietary treatments in our experiment. Compared to the control group, the addition of hen egg yolk to the rat diet regardless of its quality, adversely affected the values of HDL-C/TC and HDL-C/LDL-C in both males and females. 2023-3-15 51 59 10.2478/macvetrev-2023-0013 egg yolk plasma lipids rat dietary supplementation hen Aida Hodžić false 1 Department of Basic Veterinary Sciences, Veterinary Faculty, University of Sarajevo, Zmaja od Bosne 90, 71000 Sarajevo, Bosnia and Herzegovina AUTHOR Husein Ohran husein.ohran@vfs.unsa.ba false 2 Department of Basic Veterinary Sciences, Veterinary Faculty, University of Sarajevo, Zmaja od Bosne 90, 71000 Sarajevo, Bosnia and Herzegovina LEAD_AUTHOR Muhidin Hamamdžić false 3 Department of Basic Veterinary Sciences, Veterinary Faculty, University of Sarajevo, Zmaja od Bosne 90, 71000 Sarajevo, Bosnia and Herzegovina AUTHOR Abdulah Gagić false 4 Department of Animal Production and Biotechnology, Veterinary Faculty, University of Sarajevo, Zmaja od Bosne 90, 71000 Sarajevo, Bosnia and Herzegovina AUTHOR Amina Hrković-Porobija false 5 Department of Basic Veterinary Sciences, Veterinary Faculty, University of Sarajevo, Zmaja od Bosne 90, 71000 Sarajevo, Bosnia and Herzegovina AUTHOR Eva Pašić-Juhas false 6 Department of Basic Veterinary Sciences, Veterinary Faculty, University of Sarajevo, Zmaja od Bosne 90, 71000 Sarajevo, Bosnia and Herzegovina AUTHOR Amina Selimović false 7 Medical Faculty, University of Sarajevo, Čekaluša 19, 71000 Sarajevo, Bosnia and Herzegovina AUTHOR Atifa Ajanović false 8 Department of Basic Veterinary Sciences, Veterinary Faculty, University of Sarajevo, Zmaja od Bosne 90, 71000 Sarajevo, Bosnia and Herzegovina AUTHOR Josip Krnić false 9 Department of Basic Veterinary Sciences, Veterinary Faculty, University of Sarajevo, Zmaja od Bosne 90, 71000 Sarajevo, Bosnia and Herzegovina AUTHOR Isdadiyanto, S., Sitasiwi, A.J., Mardiati, S.M. (2020). The lipid profile of rats (Rattus norvegicus L.) induced by high fat ration after exposed to ethanolicneem (Azadirazchta indica) leaf extract. J Phys Conf Ser. 1524, 012126. 1 https://doi.org/10.1088/1742-6596/1524/1/012126 Bogoriani, N.W., Ariati, N.K. (2018). The activity of Bali Andong rhizome extract of Cordyline terminalis Kunth as a hypolipidemic agent in Wistar rats with high-cholesterol diets. eIJPPR 8(1): 75-80. 2 Wu, J.H., Micha, R., Mozaffarian, D. (2019). Dietary fats and cardiometabolic disease: mechanisms and effects on risk factors and outcomes. Nat Rev Cardiol. 16(10): 581-601 3 https://doi.org/10.1038/s41569-019-0206-1 Siri-Tarino, P.W., Sun, Q., Hu, F.B., Krauss, R.M. (2010). Saturated fat, carbohydrate, and cardiovascular disease. Am J Clin Nutr. 91(3): 502-509. 4 https://doi.org/10.3945/ajcn.2008.26285 Bos, M.B., de Vriesa, J.H.M., Feskensa, E.J.M., van Dijka, S.J., Hoelenc, D.W.M., Siebelinka, E., Heijligenbergc, R., de Groot, L.C.P.G.M. (2010). Effect of a high monounsaturated fatty acids diet and a Mediterranean diet on serum lipids and insulin sensitivity in adults with mild abdominal obesity. Nutr Metab Cardiovasc Dis. 20(8): 591-598. 5 https://doi.org/10.1016/j.numecd.2009.05.008 Kromhout, D., de Goede, J. (2014). Update on cardiometabolic health effects of ω-3 fatty acids. Curr Opin Lipidol. 25(1): 85-90. 6 https://doi.org/10.1097/MOL.0000000000000041 Siri-Tarino, P.W., Sun, Q., Hu, F.B., Krauss, R.M. (2010). Saturated fatty acids and risk of coronary heart disease: modulation by replacement nutrients. Curr Atheroscler Rep. 12(6): 384-390 7 https://doi.org/10.1007/s11883-010-0131-6 Kapoor, B., Kapoor, D., Gautam, S., Singh, R., Bhardwaj, S. (2021). Dietary polyunsaturated fatty acids (PUFAs): Uses and potential health benefits. Curr Nutr Rep. 10(3): 232-242 8 https://doi.org/10.1007/s13668-021-00363-3 Saini, R.K., Keum, Y.S. (2018). Omega-3 and omega-6 polyunsaturated fatty acids: dietary sources, metabolism, and significance - a review. Life Sci. 203, 255-267. 9 https://doi.org/10.1016/j.lfs.2018.04.049 Jiang, Z., Sim, J.S. (1993). Consumption of n-3 polyunsaturated fatty acid-enriched eggs and changes in plasma lipids of human subjects. Nutrition 9(6): 513-518 10 Simopoulos, A.P. (1999). New products from the agri-food industry: the return of n-3 fatty acids into the food supply. Lipids 34(S1Part3): S297-S301. 11 https://doi.org/10.1007/BF02562324 Lewis, N.M., Seburg, S., Flanagan, N.L. (2000). Enriched eggs as a source of n-3 polyunsaturated fatty acids for humans. Poult Sci. 79(7): 971-974 12 https://doi.org/10.1093/ps/79.7.971 FAO (2010). Fats and fatty acids in human nutrition. Report of an expert consultation. FAO FAO Food Nutr Pap. 91, 1-166 13 Franczyk-Zarow, M., Kostogrys, R., Szymczyk, B., Jawien, J., Gajda, M., Cichocki, T., Wojnar, L., Chlopicki, S., Pisulewski, P. (2008). Functional effects of eggs, naturally enriched with conjugated linoleic acid, on the blood lipid profile, development of atherosclerosis and composition of atherosclerotic plaque in apolipoprotein E and low-density lipoprotein receptor double-knockout mice (apoE/LDLR−/−). Br J Nutr. 99(1): 49-58 14 https://doi.org/10.1017/S0007114507793893 Airaodion, A.I., Ngwogu, A.C., Ekenjoku, J.A., Ngwogu, K.O. (2019). Egg yolk causes high blood pressure in Wistar rats. J BioSci Biotechnol. 11(11): 94-99 15 Bogoriani, N.W., Putra, A.A.B., Heltyani, W.E. (2019). The effect of intake duck egg yolk on body weight, lipids profile and atherosclerosis diseases in male wistar rats. IJPSR 10(2): 926-932 16 Hodzic, A., Hamamdzic, M., Gagic, A., Mihaljevic, M., Vegara, M., Krnic, J., Pasic-Juhas, E. (2008). The influence of dietary palm olein, fish oil and lard on the egg yolk and plasma lipid composition and performances of laying hens. Pol J Vet Sci. 11(1): 1-7 17 Hodzic, A., Hamamdzic, M., Gagic, A., Crnkic, C., Kadric, M., Pasic-Juhas, E., Krnic, J., Hrkovic, A. (2012). Lipid composition of liver in rats fed diets supplemented with egg yolks of modified composition. Acta Vet. 62 (4): 455-466 18 https://doi.org/10.2298/AVB1204455H Friedewald, W.T., Levy, R.I., Fredrickson, D.S. (1972). Estimation of the concentration of lowdensity lipoprotein cholesterol in plasma, without use of preparative ultracentrifuge. Clin Chem. 18(6): 499-502 19 https://doi.org/10.1093/clinchem/18.6.499 Adamopoulos, P.N., Papamichael, C.M., Zampelas, A., Moulopoulos, S.D. (1996). Cholesterol and unsaturated fat diets influence lipid and glucose concentrations in rats. Comp Biochem Physiol B Biochem Mol Biol. 113 (3): 659-663 20 https://doi.org/10.1016/0305-0491(95)02078-0 Yu, Z., Mao, C., Fu, X., Ma, M. (2019). High density lipoprotein from egg yolk (EYHDL) improves dyslipidemia by mediating fatty acids metabolism in high fat diet-induced obese mice. Food Sci Anim Resour. 39(2): 179-196 21 https://doi.org/10.5851/kosfa.2018.e38 Yang, F., Ma, M., Xu, J., Yu, X., Qiu, N. (2012). An egg-enriched diet attenuates plasma lipids and mediates cholesterol metabolism of high-cholesterol fed rats. Lipids 47(3): 269-277 22 https://doi.org/10.1007/s11745-011-3646-x Liu, A.G., Ford, N.A., Hu, F.B., Zelman, K.M., Mozaffarian, D., Kris-Etherton, P.M. (2017). A healthy approach to dietary fats: understanding the science and taking action to reduce consumer confusion. Nutr J. 16, 53 23 https://doi.org/10.1186/s12937-017-0271-4 Chen, X., Du, Y., Boni, G.F., Liu, X., Kuanga, Genga, Z. (2019). Consuming egg yolk decreases body weight and increases serum HDL and brain expression of TrkB in male SD rats. J Sci Food Agric. 99(8): 3879-3885 24 https://doi.org/10.1002/jsfa.9610 Fernandez-Robredo, P., Rodriguez, J.A., Sadabaa, L.M., Recaldea, S., Garcia-Layana, A. (2008). Egg yolk improves lipid profile, lipid peroxidation and retinal abnormalities in a murine model of genetic hypercholesterolemia. J Nutr Biochem. 19(1): 40-48 25 https://doi.org/10.1016/j.jnutbio.2006.12.020 Grundy, S.M., Denke, M.A. (1990). Dietary influences on serum lipids and lipoproteins. J Lipid Res. 31(7): 1149-1172 26 https://doi.org/10.1016/S0022-2275(20)42625-2 Aguila, M.B., Loureiro, C.C., Pinheiro, A.R., Mandarim-de-Lacerda, C.A. (2002). Lipid metabolism in rats fed diets containing different types of lipids. Arq Bras Cardiol. 78(1): 32-38 27 https://doi.org/10.1590/S0066-782X2002000100003 Farrell, D.J. (1993). Enrichment of hen eggs with n-3 long-chain fatty acids and evaluation of enriched eggs in humans. Am J Clin Nutr. 68(3): 538-544 28 https://doi.org/10.1093/ajcn/68.3.538 Yashodhara, B.M., Umakanth, S., Pappachan, J.M., Bhat, S.K., Kamath, R., Choo, B.H. (2009). Omega-3 fatty acids: A comprehensive review of their role in health and disease. Postgrad Med J. 85(1000): 84-90 29 https://doi.org/10.1136/pgmj.2008.073338 Dreon, D.M., Vranizan, K.M., Krauss, R.M., Austin, M.A., Wood, P.D. (1990). The effect of polyunsaturated fat vs monounsaturated fat on plasma lipoproteins. JAMA 263(18): 2462-2466 30 https://doi.org/10.1001/jama.263.18.2462 Harris, W.S., Dayspring, T.D., Moran, T.J. (2013). Omega-3 fatty acids and cardiovascular disease: new developments and applications. Postgrad Med. 125(6): 100-113 31 https://doi.org/10.3810/pgm.2013.11.2717 Kuang, H., Yang, F., Zhang, Y., Wang, T., Chen, G. (2018). The impact of egg nutrient composition and its consumption on cholesterol homeostasis. Cholesterol 2018: 6303810 32 https://doi.org/10.1155/2018/6303810