ORIGINAL_SCIENTIFIC_ARTICLE β-lactamases are a diverse class of enzymes produced by bacteria that present a major cause for resistance to β-lactams.In this study we analysed 159 fecal samples from dairy cows, for the presence of presumptive ESBL, AmpC, and carbapenemase-producing E. coli. Phylotyping was done using Clermont phylo-typing method, targeting arpA, ChuA, and YjaA genes, along with the DNA fragment TspE4.C2. Convetional PCR method was used to confirm the presence of bla genes among 39 phenotypically confirmed ESBL producing E. coli. The results showed presence of CTX-M, SHV, TEM and OXA1 bla genes in 28 (71.79%), 1 (2.56%), 29 (74.35%), 2 (5.12%) of isolates, respectively Twenty (51.28%) isolates showed presence of both blaCTX-M and TEM genes. The strain that carried the blaSHV gene was found to carry blaTEM gene as well, while one of the strains that carried blaOXA1 gene was also carrying blaCTX-M and TEM gene. The ration between isolates and phylo-groups was as follows: 9 (23.07%) strains were assigned to phyllo-group D; 14 (35.89%) to phyllo-group B; 16 (41.02%) to phyllo-group A. Out of the 39 strains where bla genes were identified, 29 (74.35%) were categorized as multi drug resistant. https://macvetrev.mk/LoadArticlePdf/365 2023-3-15 89 97 10.2478/macvetrev-2023-0017 ESBL E. coli bla genes phylogenetic groups dairy cow Maksud Kerluku false 1 Veterinary Practice Maxi, Amdi Leshi 21, 1250 Debar, North Macedonia AUTHOR Dean Jankuloski intanshameha@upm.edu.my false 2 Faculty of Veterinary Medicine-Skopje, Ss. Cyril and Methodius University in Skopje,Lazar Pop-Trajkov 5-7, 1000 Skopje, North Macedonia AUTHOR Marija Ratkova-Manovska false 3 Faculty of Veterinary Medicine-Skopje, Ss. Cyril and Methodius University in Skopje,Lazar Pop-Trajkov 5-7, 1000 Skopje, North Macedonia AUTHOR Mirko Prodanov false 4 Faculty of Veterinary Medicine-Skopje, Ss. Cyril and Methodius University in Skopje,Lazar Pop-Trajkov 5-7, 1000 Skopje, North Macedonia AUTHOR Biljana Stojanovska-Dimzoska false 5 Faculty of Veterinary Medicine-Skopje, Ss. Cyril and Methodius University in Skopje,Lazar Pop-Trajkov 5-7, 1000 Skopje, North Macedonia AUTHOR Aleksandar Dodovski false 6 Faculty of Veterinary Medicine-Skopje, Ss. Cyril and Methodius University in Skopje,Lazar Pop-Trajkov 5-7, 1000 Skopje, North Macedonia AUTHOR Katerina Blagoevska katerinab@fvm.ukim.edu.mk false 7 Faculty of Veterinary Medicine-Skopje, Ss. Cyril and Methodius University in Skopje,Lazar Pop-Trajkov 5-7, 1000 Skopje, North Macedonia LEAD_AUTHOR Hosain, M.Z., Kabir, S.M.L., Kamal, M.M. (2021).Antimicrobial uses for livestock production in developing countries. Vet World. 14(1): 210-221. PMid:33642806 PMCid:PMC7896880. 1 https://doi.org/10.14202/vetworld.2021.210-221 Serwecińska, L. (2020). Antimicrobials and antibiotic-resistant bacteria: a risk to the environment and to public health. 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