Short Communication This descriptive study aims to examine the behavior of dogs diagnosed with hypothyroidism and the potential effect of hormonal treatment. Eight client-owned dogs with clinical hypothyroidism were evaluated using an adapted C-BARQ questionnaire, clinical description, and hematological analysis. Six of the dogs’ behavior was monitored for four months after the treatment initiation. The study found that excitation, whining, and urinating when left alone were frequently observed. Attention-seeking was also a common behavior reported by the owners. The biochemical analysis before the treatment had revealed elevated cholesterol, triglycerides, and alkaline phosphatase levels in the majority of dogs. The study emphasizes the need for prospective studies using a larger sample size to gain further insight into the relationship between hypothyroidism and behavior in dogs. Monitoring changes in behavior over time can provide insight into how thyroid dysregulation may contribute to the onset of certain behavioral patterns. Functional brain imaging and pathohistological brain analysis in dogs with long-term hypothyroidism are also required to confirm the effects of hypothyroidism on canine brain function. https://macvetrev.mk/LoadArticlePdf/361 2023-6-27 185 191 https://doi.org/10.2478/macvetrev-2023-0021 Canine hypothyroidism behavior clinical presentation endocrinology hematology Elena Mitrevska e.mitrevska@fvm.ukim.edu.mk false 1 Faculty of Veterinary Medicine-Skopje, Ss. Cyril and Methodius University in Skopje, Lazar Pop-Trajkov 5-7, 1000 Skopje, N. Macedonia LEAD_AUTHOR Irena Celeska false 2 Faculty of Veterinary Medicine-Skopje, Ss. Cyril and Methodius University in Skopje, Lazar Pop-Trajkov 5-7, 1000 Skopje, N. Macedonia AUTHOR Miroslav Kjosevski false 3 Faculty of Veterinary Medicine-Skopje, Ss. Cyril and Methodius University in Skopje, Lazar Pop-Trajkov 5-7, 1000 Skopje, N. Macedonia AUTHOR Elena Atanaskova Petrov false 4 Faculty of Veterinary Medicine-Skopje, Ss. Cyril and Methodius University in Skopje, Lazar Pop-Trajkov 5-7, 1000 Skopje, N. Macedonia AUTHOR O’Neill, D.G., Khoo, J.S.P., Brodbelt, D.C., Church, D.B., Pegram, C., Geddes, R.F. (2022). Frequency, breed predispositions and other demographic risk factors for diagnosis of hypothyroidism in dogs under primary veterinary care in the UK. Canine Med Genet. 9, 11. PMid:36217196 PMCid:PMC9552398 1 https://doi.org/10.1186/s40575-022-00123-8 Dixon, M., Reid, S.W., Mooney, C.T. (1999). Epidemiological, clinical, hematological and biochemical characteristics of canine hypothyroidism. Vet Rec. 145(17): 481-487. PMid:10596870 2 https://doi.org/10.1136/vr.145.17.481 Bertalan, A., Kent, M., Glass, E. (2013). Neurologic manifestations of hypothyroidism in dogs. Compend Contin Educ Vet. 35(3): E2. 3 Panciera, D.L. (2001). Conditions associated with canine hypothyroidism. Vet Clin North Am Small Anim Pract. 31(5): 935-950. 4 https://doi.org/10.1016/S0195-5616(01)50006-6 Mooney, C.T., Peterson, M.E. (2004). Canine hypothyroidism. In C.T. Mooney, M.E. Peterson (Eds.), BSAVA manual of canine and feline endocrinology (pp. 63-85). British Small Animal Veterinary Association PMCid:PMC6065554 5 https://doi.org/10.22233/9781905319893.8 Scott-Moncrieff, J.C. (2007). Clinical signs and concurrent diseases of hypothyroidism in dogs and cats. Vet Clin North Am Small Anim Pract. 37(4): 709-722. PMid:17619007 6 https://doi.org/10.1016/j.cvsm.2007.03.003 Roopali, B., Roy, S., Roy, M., Ali, S.L. (2020). Heamatological alterations in hypothyroidism dogs. Pharm Innov J. SP-9(9): 49-52. 7 https://doi.org/10.20546/ijcmas.2020.903.167 Shahid, M.A., Ashraf, M.A., Sharma, S. (2022). Physiology, thyroid hormone. [Updated 2023 May 1]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing 8 Aronson, L.P., Dodds, W.J. (2005). The effect of hypothyroid function on canine behavior. In: D. Mills, E. Levine, G. Landsberg, D. Horwitz, M. Duxbury, P. Mertens, K. Meyer, L.R. Huntley, M. Reich, J. Willard (Eds.), Current issues and research in veterinary behavioral medicine, Papers presented at the 5th international veterinary behavior meeting, (pp.131-138). West Lafayette, Indiana: Purdue University Press 9 Schreuder, B., de John, M., Pekelder, J.J., Vellema, P. (2002). Four cases of aggression and hypothyroidism in dogs. Vet Rec. 151(18): 547-548. PMid:12448494 10 https://doi.org/10.1136/vr.151.18.547 Manteca, X. (2002). Difficulties in the diagnosis of dominance aggression in dogs. Proceedings of the 27 WSAVA Congress. Autonomous University of Barcelona, Spain 11 Hrovat, A., De Keuster, T., Kooistra, H.S., Ducheteau, L., Oyama, M.A., Peremans, K., Damient, S. (2019). Behavior in dogs with spontaneous hypothyroidism during treatment with levothyroxine. J Vet Intern Med. 33(1): 64-71. PMid:30499213 PMCid:PMC6335523 12 https://doi.org/10.1111/jvim.15342 Radosta, L.A., Shofer, F.S. Reisner, I.R. (2012). Comparison of thyroid analytes in dogs aggressive to familiar people and in non-aggressive dogs. Vet J. 192(3) : 472-475. PMid:21795082 13 https://doi.org/10.1016/j.tvjl.2011.06.029 Lin, C., Li, N., Chang, H., Shen, Y., Li, Z., Wei, W., Chen, H., et al. (2020). Dual effects of thyroid hormone on neurons and neurogenesis in traumatic brain injury. Cell Death Dis. 11(8): 671. PMid:32826870 PMCid:PMC7442821 14 https://doi.org/10.1038/s41419-020-02836-9 Pilhatsch, M., Marxen, M., Winter, C., Smolka, M.N., Bauer, M. (2011). Hypothyroidism and mood disorders: integrating novel insights from brain imaging techniques. Thyroid Res. 4(Suppl 1): S3. PMid:21835050 PMCid:PMC3155109 15 https://doi.org/10.1186/1756-6614-4-S1-S3 Yin, X.L. (2021). Changes of structural and functional attention control networks in subclinical hypothyroidism. Front Behav Neurosci. 15, 725908. PMid:34776889 PMCid:PMC8585844 16 https://doi.org/10.3389/fnbeh.2021.725908 Lucia, F.S., Pacheco-Torres, J., González-Granero, S., Canals, S., Obregón, M.J., García-Verdugo, J.M., Berbel, P. (2018). Transient hypothyroidism during lactation arrests myelination in the anterior commissure of rats. A magnetic resonance image and electron microscope study. Front Neuroanat. 12, 31. PMid:29755326 PMCid:PMC5935182 17 https://doi.org/10.3389/fnana.2018.00031 Mooney, C.T. (2011). Canine hypothyroidism: a review of aetiology and diagnosis. N Z Vet J. 59(3): 105-114. PMid:21541883 18 https://doi.org/10.1080/00480169.2011.563729 Nishii, N., Okada, R., Matsuba, M., Takashima, S., Kobatake, Y., Kitagawa, H. (2019). Risk factors for low plasma thyroxine and high plasma thyroidstimulating hormone concentrations in dogs with non-thyroidal diseases. J Vet Med Sci. 81(8): 1097-1103. PMid:31204368 PMCid:PMC6715931 19 https://doi.org/10.1292/jvms.19-0169 Canejo-Teixeira, R., Almiro, P.A., Serpell, J.A., Baptista, L.V. Niza, M.M. (2018). Evaluation of the factor structure of the Canine Behavioural Assessment and Research Questionnaire (C-BARQ) in European Portuguese. PloS One 13(12): e0209852. PMid:30589886 PMCid:PMC6307869 20 https://doi.org/10.1371/journal.pone.0209852 Beaver, B.V., Haug, L.I. (2003). Canine behaviors associated with hypothyroidism. J Am Anim Hosp Assoc. 39(5): 431-434. PMid:14518649 21 https://doi.org/10.5326/0390431 Dixon, R.M., Mooney, C.T. (1999). Evaluation of serum free thyroxine and thyrotropin concentrations in the diagnosis of canine hypothyroidism. J Small Anim Pract. 40(2): 72-78. PMid:10088086 22 https://doi.org/10.1111/j.1748-5827.1999.tb03040.x Bennaim, M., Shiel, R.E., Evans, H. Mooney, C.T. (2022). Free thyroxine measurement by analogue immunoassay and equilibrium dialysis in dogs with non-thyroidal illness. Res Vet Sci. 147, 37-43. PMid:35430462 23 https://doi.org/10.1016/j.rvsc.2022.03.016