Original Scientific Article Serratiopeptidase is a zinc-containing metalloprotease primarily obtained from Serratia marcescens isolated from the silkworm guts. This study aimed to assess the antinociceptive, anti-inflammatory, and antipyretic effects of serratiopeptidase in hen chicks. It included 104 hen chicks weighing 70-90 g. The antinociception efficacy was assessed by electricalstimulation and hot-water test. Anti-inflammatory efficacy was assessed by formalin test. Assessment of therapeutic and antipyretic efficacy was determined by Baker’s yeast-induced pyrexia test. In the electrical-stimulation test, 20 and 40 mg/kg of serratiopeptidase induced an antinociceptive effect in 15% and 18%, respectively. In the hot-water test, this effect was observed in 31 and 82%, respectively. In the first phase of the formalin test, an antinociceptive effect was observed yeast. 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