Original Scientific Article There is no known artificial breeding method for depik fish Rasbora tawarensis. Sperm extender and the dilution ratio are crucial factors in the artificial breeding of fish. The current study aimed to assess the effect of several common fish sperm extenders and its dilution ratio on sperm characteristics and fertilization of depik fish eggs. Two series of experiments were performed to find the best extender and its dilution ratio. In the first experiment, five types of extenders were tested: Ringer’s solution, physiological solution, coconut water, sugarcane water, and roomie water. The second experiment studied four dilution levels of sperm and extender (v/v): 1:20, 1:30, 1:40, and 1:50. Every treatment was conducted in four replicates and the experiments were conducted consequently so that the findings in the first experiment were applied in the second. The first experiment showed that Ringer’s is the best extender for depik sperm with sperm motility, fertilization and hatching rates of 71.00%, 69.30%, and 53.66%, respectively; and the second experiment revealed that Ringer’s at dilution ratio 1:40 gave higher sperm motility, fertilization, and hatching rates of 74.66, 70.33, and 59.00%, respectively. In conclusion, Ringer’s solution was the most favorable extender for sperm which yielded best fertilization results at 1:40 dilution ratio (sperm:extender, v/v) in depik fish. https://macvetrev.mk/LoadArticlePdf/361 2024-10-15 123 129 https://doi.org/10.2478/macvetrev-2024-0022 depik fish sperm fertilization Ringer’s solution physiological solution Zainal Abidin Muchlisin muchlisinza@usk.ac.id false 1 Department of Aquaculture, Faculty of Marine and Fisheries, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia LEAD_AUTHOR Dhea Febby Aldila false 2 Department of Aquaculture, Faculty of Marine and Fisheries, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia AUTHOR Putri Inten Sarah false 3 Department of Aquaculture, Faculty of Marine and Fisheries, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia AUTHOR Kartini Eriani false 4 Department of Biology, Faculty of Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia AUTHOR Iwan Hasri false 5 Veterinary and Fisheries Affair of Aceh Tengah District, Takengon, Indonesia AUTHOR Nur Fadli false 6 Department of Aquaculture, Faculty of Marine and Fisheries, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia AUTHOR Abdullah Abbas Muhammadar false 7 Department of Aquaculture, Faculty of Marine and Fisheries, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia AUTHOR Luvi Syafrida Handayani false 8 Department of Aquaculture, Faculty of Marine and Fisheries, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia AUTHOR Siti Maulida false 9 Department of Aquaculture, Faculty of Marine and Fisheries, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia AUTHOR Mohd Nor Siti-Azizah false 10 Institute Marine Biotechnology and Climate Changes, Universiti Malaysia Terengganu, Malaysia AUTHOR Filiz Kutluyer Kocabas false 11 Faculty of Fishery, Munzur University, Turkey AUTHOR Mehmet Kocabas false 12 Faculty of Life Science, Karadeniz Technical University, Turkey AUTHOR Muchlisin, Z.A., Fadli, N., Rudi, E., Mendo, T., Siti-Azizah, M.N. 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