Original Scientific Article Biosecurity measures, coupled with effective on-farm management, encompass all aspects aimed at preventing the entry of pathogens into pig farms and their subsequent spread within the facility. This study assessed biosecurity practices on commercial pig farms in Serbia by surveying farm veterinarians/managers. To evaluate biosecurity on these farms, a Biocheck online survey (https://biocheckgent.com) was conducted across ten commercial pig farms in Northern Serbia, where the highest concentration of industrial pig production occurs. The average overall biosecurity score was 65.56 ±11.65. External biosecurity scored higher (75.56 ±9.39), whereas internal biosecurity scored lower (54.89 ±16.92). Within external biosecurity, the weakest scores were observed in categories such as feed, water, and equipment supply (47 ±25.48), showing considerable variation among farms, particularly in feed, water, equipment supply (54.22), and farm location (57.65). Regarding internal biosecurity, the lowest scores were noted during the farrowing and suckling period (37.22 ±15.08) and the finishing unit (37.66 ±24.12). This study reveals that biosecurity measures in commercial pig farms in Serbia are slightly below the global average. Given the predominance of extensive production systems in Serbia, urgent improvements in biosecurity practices on industrial farms are essential. Identification and implementation of appropriate biosecurity measures tailored to existing animal production systems are urgently needed. https://macvetrev.mk/LoadArticlePdf/361 2024-10-15 141 149 https://doi.org/10.2478/macvetrev-2024-0025 biosecurity assessment pig commercial (industrial) farms Serbia Branislav Kureljušić branislavkureljusic@yahoo.com false 1 Scientific Institute of Veterinary Medicine of Serbia, Janisa Janulisa 14, 11000 Belgrade, Republic of Serbia LEAD_AUTHOR Jelena Maletić false 2 Scientific Institute of Veterinary Medicine of Serbia, Janisa Janulisa 14, 11000 Belgrade, Republic of Serbia AUTHOR Božidar Savić false 3 Scientific Institute of Veterinary Medicine of Serbia, Janisa Janulisa 14, 11000 Belgrade, Republic of Serbia AUTHOR Bojan Milovanović false 4 Scientific Institute of Veterinary Medicine of Serbia, Janisa Janulisa 14, 11000 Belgrade, Republic of Serbia AUTHOR Milan Ninković false 5 Scientific Institute of Veterinary Medicine of Serbia, Janisa Janulisa 14, 11000 Belgrade, Republic of Serbia AUTHOR Nemanja Jezdimirović false 6 Scientific Institute of Veterinary Medicine of Serbia, Janisa Janulisa 14, 11000 Belgrade, Republic of Serbia AUTHOR Jasna Prodanov Radulović false 7 Scientific Veterinary Institute “Novi Sad”, Rumenački put 20, 21000 Novi Sad, Republic of Serbia AUTHOR Alarcón, L.V., Allepuz, A., Mateu, E. 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