Original Scientific Article Rabies is controlled by mass animal vaccination campaigns. Cats, dogs, and wild animals (e.g., bats) are large reservoirs of this virus and can pose a significant threat to the human health, especially in the developing countries. The nucleoprotein of the rabies virus is of great scientific interest since it has the potential to generate immunity in animals and can be used as for immunochemical diagnostics. The study aimed to test a large-scale expression of the rabies N protein in a prokaryotic system. The recombinant N protein was successfully expressed and purified. It was immunologically recognized by specific antibodies and was able to induce the production of specific antibodies in a mouse immunization assay. These encouraging results indicate that the recombinant N protein can be evaluated as an antigen for the development of a subunit vaccine or for a diagnostic assay. https://macvetrev.mk/LoadArticlePdf/361 2024-10-15 151 158 https://doi.org/10.2478/macvetrev-2024-0026 rabies virus nucleoprotein Escherichia coli large-scale expression Leandro Daniel Picotto false 1 1Reproductive Biotechnology, National Agricultural Technology Institute, (INTA), Ruta 226 km 73.5, 7620, Balcarce, Argentina AUTHOR Carlos Javier Panei javierpanei@fcv.unlp.edu.ar false 2 Virology Laboratory, Faculty of Veterinary Sciences, National University of La Plata, 60& 118, CC 296, 1900, La Plata, Argentina; National Scientific and Technical Research Council (CONICET), Argentina LEAD_AUTHOR Marcelo Ricardo Pecoraro false 3 Virology Laboratory, Faculty of Veterinary Sciences, National University of La Plata, 60& 118, CC 296, 1900, La Plata, Argentina AUTHOR Guillermo Hernán Sguazza false 4 Virology Laboratory, Faculty of Veterinary Sciences, National University of La Plata, 60& 118, CC 296, 1900, La Plata, Argentina AUTHOR Martinez, L. 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