Original Scientific Article This work aimed to evaluate the lactoferrin (LF) effect on arsenic (As) and imidacloprid (IMI) toxicity in broiler chicks. One-week old broiler chicks (n=105) were divided into seven groups (x15 each). The animals were orally supplemented with As, IMI, and/or LF for 4 weeks as follows: Control (G1) no supplements, G2 supplemented with As, G3 supplemented with IMI, G4 supplemented with As+IMI, G5 supplemented with As+LF, G6 supplemented with IMI+LF, G7 supplemented with As+IMI+LF. Body weight and weight gain were recorded on weekly interval. Blood, serum, liver, kidney, and muscle samples were collected at the end of the experimental period for biochemical and histopathological examination. Body weight performance, hematological, serum, and liver tissue biochemical analysis revealed adverse changes in G2, G3, and G4 compared to control, G5, G6, and G7. There was higher tissue residue of As and IMI in G4 and G5 compared to G5, G6, and G7. Liver histopathological changes in the groups supplemented with As and/or IMI were observed with necrosis, congestion, and inflammatory cell aggregates. The use of LF in broiler chicks improves weight gain performance and modulates the adverse effects of As and/or IMI toxicity. https://macvetrev.mk/LoadArticlePdf/361 2024-10-15 167 178 https://doi.org/10.2478/macvetrev-2024-0028 lactoferrin effect arsenic toxicity imidacloprid toxicity histopathology broilers chicks Marwa Fouad Hassan false 1 Department of Biochemistry, Toxicology and Feed Deficiency, Animal Health Research Institute (AHRI), Agricultural Research Center (ARC), Dokki, Giza, Egypt AUTHOR Asmaa Gamal Abd El Monsef false 2 Department of Biochemistry, Toxicology and Feed Deficiency, Animal Health Research Institute (AHRI), Zagazig Branch, Agricultural Research Center (ARC), Zagazig, Egypt AUTHOR Nermin Farouq El Zohairy false 3 Department of Biochemistry, Toxicology and Feed Deficiency, Animal Health Research Institute (AHRI), Zagazig Branch, Agricultural Research Center (ARC), Zagazig, Egypt AUTHOR Sanaa Mohamed Salem false 4 Department of Pathology, Animal Health Research Institute (AHRI), Zagazig Branch, Agricultural Research Center (ARC), Zagazig, Egypt AUTHOR Safaa Mohamed Elmesalamy false 5 Department of Biochemistry, Toxicology and Feed Deficiency, Animal Health Research Institute (AHRI), Zagazig Branch, Agricultural Research Center (ARC), Zagazig, Egypt AUTHOR Hamada Mahmoud Yousif hamadayousif82@gmail.com false 6 Department of Biochemistry, Toxicology and Feed Deficiency, Animal Health Research Institute (AHRI), Agricultural Research Center (ARC), Dokki, Giza, Egypt LEAD_AUTHOR Mogda Kamel Mansour false 7 Department of Biochemistry, Toxicology and Feed Deficiency, Animal Health Research Institute (AHRI), Agricultural Research Center (ARC), Dokki, Giza, Egypt AUTHOR Valskys, V., Hassan, H.R., Wołkowicz, S., Satkūnas, J., Kibirkštis, G., Ignatavičius, G. 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