ORIGINAL_ARTICLE
The prevalence of Pasteurella multocida from farm pigs in Serbia
The investigations covered a total of 234 lungs from necropsied pigs with different pneumonic lesions, from 6 farrow-to-finish pig farms during 2013 and 2014. The samples were inoculated on selective culture media and aerobically incubated at 37oC and in carbon dioxide condition. The isolated bacterial colonies were further characterised morphologically and biochemically. The identification was confirmed using the BBL Crystal, E/N, G/P ID Kit (Becton Dickinson). For determination of the type of Pasteurella multocida, the PCR method was used. The findings showed that bacteria were isolated from 202 (86%) out of 234 examined lung samples. The pure isolates of Pasteurella multocida were obtained from 71 (35 %) samples. Out of the remaining 29 (14%) examined lung samples, 9, 8, 7 and 5 examined lung samples were shown as mixed cultures of Pasteurella multocida and Streptococcus spp., Arcanobacterium pyogenes, Actinobacillus pleuropneumoniae and Haemophilus parasuis, respectively. The PCR method confirmed that all 15 investigated strains of P. multocida belong to type A.
https://macvetrev.mk/Files/Article/2020/10.1515/macvetrev-2015-0063/macvetrev-2015-0063.pdf
2016-03-15T09:00:00
39
42
10.2478/macvetrev-2015-0063
Pasteurella multocida
lung
pig
Oliver
Radanovic
radanovic.oliver@gmail.com
false
1
Institute of Veterinary Medicine of Serbia, Vojvode Toze 14, 11000 Belgrade, Serbia
LEAD_AUTHOR
Jadranka
Zutic
false
2
Institute of Veterinary Medicine of Serbia, Vojvode Toze 14, 11000 Belgrade, Serbia
AUTHOR
Dobrila
Jakic-Dimic
false
3
Institute of Veterinary Medicine of Serbia, Vojvode Toze 14, 11000 Belgrade, Serbia
AUTHOR
Branislav
Kureljusic
false
4
Institute of Veterinary Medicine of Serbia, Vojvode Toze 14, 11000 Belgrade, Serbia
AUTHOR
Bozidar
Savic
false
5
Institute of Veterinary Medicine of Serbia, Vojvode Toze 14, 11000 Belgrade, Serbia
AUTHOR
Bochev, I. (2007). Porcine respiratory disease complex (PRDC): A review. I. Etiology, epidemiology, clinical forms and pathoanatomical features. Bulg. J. Vet. Med. 10, 131-146.
1
Savić, B., Radanović, O., Žutić, M., Pavlović, I., Jakić-Dimić, D. (2011). Prevalenca bakterijskih uzročnika pneumonija svinja. IX Simpozijum Veterinara “Zdravstvena zaštita, selekcija i reprodukcija svinja”. Zbornik radova. 77-82. Srebreno Jezero.
2
Woeste, K. Grosse, E. (2007). Transmission of agents of the porcine respiratory disease complex (PRDC) between swine herds; a review. Part 2-Pathogen transmission via semen air and living/nonliving vectors. Deutsche. Tierarzt. Wochenschrift. 114, 364-373. PMid:17970334
3
Baker, R.B. (2005). Diseases of the respiratory system. Proceedings of the North Carolina healthy hogs seminar. Clinton. 1-19. PMCid:PMC4147871
4
Halbur, P.G. (1997). Porcine respiratory disease complex, Proceedings of the North Carolina healthy hogs seminar. 1-15.
5
Lee, A. (2013). Mycoplasmal pneumonia in pigs, 1-3. http://www.dpi.nsw.gov.au
6
Tigga, M., Ghosh, R.C., Malik, P., Choudhary, B.K., Tigga, P., Nagar, D.K. (2014). Isolation, characterization, antibiogram and pathology of Pasteurella multocida isolated from pigs, Vet. World 7, 363-368.
7
10.14202/vetworld.2014.363-368
Jaglic, Z., Kucerova, Z., Nedbalcova, K., Pavlik, I., Alexa, P., Bartos, M. (2005). Characterisation and comparison of Pasteurella multocida isolated from different species in the Czech Republic: capsular PCR typing, ribotyping and dermonecrotoxin production. Vet. Med. – Czech. 50 (8): 345–354
8
Dutra, V., Chitarra, C., Paula, D.A. (2013). Detection of Pasteurella multocida by qPCR Associated with Ppneumonic lung in pigs slaughtered in M Grosso Brazil. Int. J. Sc. 2,25-31.
9
Holko, I., Urbanova, J., Holkova, T., Kmet, V. (2004). Diagnostics of main bacterial agents of porcine respiratory diseases complex (PRDC) using PCR detection of Mycoplasma hyopneumoniae. Vet. Med. – Czech. 49, 35–41.
10
Radanović, O., Savić, B., Kureljušić, B., Ivetić, V., Žutić, M. (2010). Učestalost izolacije Pasteurella multocida iz pluća prasadi iz kategorije odgoja.Proceedings of the XII Symposium of Epidemiological days with international participation. Oplenac-Topola. 99-102.
11
Stepniewska, K., Markowska-Daniel, I. (2013). Phenotypic and genotypic characterization of Pasteurella multocida strains isolated from pigs in Poland. Bull Vet Inst Pulawy 57, 29-34.
12
Radanović, O., Žutić, M., Ivetić, V., Savić, B., Stanojević, S. (2008). Significance of Pasteurella multocida in respiratory disease complex of swine. Proceedings of the X Symposium of Epidemiological days with international participation. Tara. 253-255.
13
Žutić, M., Ivetić, V., Radanović, O., Žutić, J., Jakić-Dimić, D., Savić, B., Pavlović, I., Stanojević, S. (2009). Investigations of representation of certain bacteria strains in lungs of pigs with pneumonia. Vet. Glasnik 63, 3-15.
14
10.2298/VETGL0902003Z
Choi, Y.K., Goya, S.M., Joo, H.S. (2003). Retrospective analysis of etiological agents associated with respiratory diseases in pigs. Can. Vet. J. 44, 735-737. PMid:14524628 PMCid:PMC340270
15
Hansen, M.S., Pors, S.E., Jensen, H.E., Bille-Hansen V., Bisgard, M., Flachs, E.M., Nielsen O.L. (2010). An investigation of the pathology and pathogens associated with porcine respiratory disease complex. in Denmark. J. Comp. in Denmark. J. Comp. Pathology. 143,120-131. PMid:20181357
16
10.1016/j.jcpa.2010.01.012
Opriessnig, T., Gimenez-Lirola, L.G., Halbur, P.G. (2011). Polymicrobial respiratory disease in pigs. Anim. Health. Res. Rev. 12,133-48. PMid:22152290
17
10.1017/S1466252311000120
Weissenbacher-Lang, C., Kureljušić, B., Nedorost, N., Stixenberger, D., Binanti, D., Viehmann, M., Weissenböck, H. (2014). Association between Pneumocystis carinii and bacterial lung pathogens in pigs with pneumonia. Proc. 23rd IPVS Congress,Cancún, Mexico, 08.06.–11.06.2014. p. 53.
18
Pors, S.E., Hensen, M.S., Christensen, H., Jensen, H.E., Petersen, A., Bisgard, M. (2011). Genetic diversity and associated pathology of Pasteurella multocida isolated from porcine pneumonia. Vet. Microbiol. 150, 354-361
19
10.1016/j.vetmic.2011.02.050
Petrović, T., Miličević, V., Radulović-Prodanov, J., Maksimović-Zorić, J., Lupulović, D., Došen, R., Lazić, S. (2011). Molecular detection and genetic analysis of Serbian PRRSV isolates. Euro PRRS Symposium. Proceedings pp. 50-56. 12-14 October Novi Sad.
20
Truszczyński, M., Pejsak, Z. (2010). Immunosuppression as the cause of swine diseases of multifactorial etiology. Meycyna Wet. 66, 370-373.
21