Mac Vet Rev UKIM-FVMS Macedonian Veterinary Review : Mac Vet Rev 1409-7621 1857-7415 Faculty of Veterinary Medicine - Skopje https://doi.org/10.2478/macvetrev-2025-0027 macvetrev-2025-0027 Original Scientific Article Echocardiographic and hematological changes in dogs naturally infected with Ehrlichia spp. https://orcid.org/0009-0000-1718-2216 Ustaer Zeynep 1 https://orcid.org/0000-0002-9873-0364 Özalp Tahir 2 https://orcid.org/0000-0002-7833-5519 Erdogan Songül 3 https://orcid.org/0000-0003-1867-7143 Ural Kerem 4 https://orcid.org/0000-0001-5141-5108 Erdogan Hasan 5 15 10 2025 31 07 2025 48 2 i x 01 11 2024 13 06 2025 27 06 2025 © 2025 Ustaer Z. This is an open-access article published under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 2025 https://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. This study aimed to assess the severity of anemia, associated parameters, and their impact on cardiac function in dogs naturally infected with Ehrlichia canis (E. canis), a zoonotic pathogen primarily transmitted by the brown dog tick. Forty-seven dogs, comprising 38 infected and 9 healthy controls, were classified into non-anemic (n=12), mildly anemic (n=9), moderately anemic (n=11), severely anemic (n=4), and very severely anemic (n=2) groups. Hematological and echocardiographic assessments were performed, along with separate measurements of cardiac biomarkers (e.g., cardiac troponin I [cTnI]) and iron metabolism biomarkers (e.g., serum iron, total iron-binding capacity [TIBC], and transferrin saturation). Hematological analysis revealed a significant reduction in hematocrit (17.11±1.34%), hemoglobin (5.22±0.88 g/dL), and platelet counts (38.25±22.62×109/L) in severely anemic dogs compared to other groups. Echocardiographic findings demonstrated increased interventricular septal thickness during diastole (1.16±0.60 cm) and systole (1.38±0.10 cm) in moderately anemic dogs, indicating compensatory adaptations. Dogs with severe anemia exhibited elevated left ventricular end-diastolic (3.56±0.54 cm) and end-systolic dimensions (1.71±0.22 cm) in comparison to control dogs (p<0.05). Fractional shortening (53.83±7.71%) and ejection fraction (77.58±8.19%) were diminished among the severely anemic group, denoting altered cardiac function. Elevated cardiac troponin I (cTnI) levels (up to 0.29±0.22 ng/mL) were noted in a limited number of cases, suggesting that myocardial injury was not widespread. This study demonstrated that anemia severity significantly influences myocardial and hematological parameters in E. canis-infected dogs, with evidence indicating compensatory cardiac remodeling in response to chronic anemia. Further research using advanced diagnostic techniques is recommended to better understand disease progression and associated complications. Ehrlichiosis dogs haematology biochemistry echocardiography INTRODUCTION

Ehrlichiosis is a multisystemic disease that affects various organs and tissues, including the heart. Recent studies continue to focus on the cardiac effects of canine monocytic ehrlichiosis (CME). Gal et al. (2008) reported that E. canis spreads to multiple organs, including the heart 1. Varshney et al. (2015) suggested an increased risk of myocardial damage in acute CME cases 2, while Charaborty et al. 3 found that CME cases exhibited macroscopic and microscopic hemorrhages in the heart. Kumar and Varshney 4 identified cardiac arrhythmias in canine ehrlichiosis but were unable to confirm the presence of myocardial damage.

Co-infections involving Ehrlichia and Babesia or Trypanosoma have also been associated with significant arrhythmias 4, 5. Electrocardiographic abnormalities such as sinus tachycardia, atrial fibrillation, second-degree Mobitz type B block, ventricular premature contractions (VPC), and ST changes are commonly found in dogs infected with CME 6. Similar findings have been observed in human ehrlichiosis patients 7.

The arrhythmias and conduction abnormalities in acute ehrlichiosis are thought to be linked to hypoxia and subsequent metabolic changes resulting from hemodynamic alterations 4, 5. Severe arrhythmias, such as atrial fibrillation or multiple pathological electrocardiographic changes, may increase the risk of death in ehrlichiosis cases, emphasizing the need for prompt intervention. Despite the presence of arrhythmias in acute CME cases, electrocardiography (ECG) alone may not be sufficient to detect minor myocardial damage. Hence, cardiac biomarkers and echocardiography are crucial for early detection 8. Biomarkers such as serum cardiac troponin I (cTnI), a sensitive indicator of myocardial injury, have been studied in ehrlichiosis-infected dogs. Elevated cTnI levels were observed in a significant number of cases, which showed a marked decrease after 14 days of treatment, suggesting that cardiac damage in E. canis infection may be reversible 8.

Additionally, a study reported echocardiographic abnormalities in 33% of mono-infected, 27% of co-infected, and 34% of PCR-negative dogs, with left ventricular dilation and increased wall thickness correlated with elevated cTnI levels 8. Anemia, which commonly affects multiple organs, can significantly influence cardiac function. Its impact is determined by factors such as anemia severity, onset rate, compensatory mechanisms, and the patient's physiological status 9. Red blood cells play a key role in oxygen transport, and any imbalance can lead to reduced oxygen delivery, which the body attempts to compensate through mechanisms such as increased cardiac output and enhanced erythropoietin production 10, 11.

The present study aimed to evaluate the severity of anemia, its related parameters, and its effects on cardiac function in dogs infected with E. canis, a zoonotic pathogen of increasing importance. We hypothesized that the severity of anemia in E. canis-infected dogs is associated with progressive echocardiographic and hematological alterations, reflecting compensatory cardiac remodeling and systemic involvement.

 MATERIAL AND METHODS <italic>Animal subjects and grouping</italic>

A total of 47 dogs were included in this study, consisting of 38 infected dogs and 9 healthy control dogs. The infected dogs, with no known history of cardiac disease, were presented to the Internal Medicine Clinic of the Faculty of Veterinary Medicine, Aydın Adnan Menderes University. These dogs exhibited clinical signs characteristic of different stages of canine monocytic ehrlichiosis (CME), such as fever, lethargy, anorexia, generalized lymphadenopathy, and epistaxis. The infected dogs were categorized based on anemia severity into five subgroups: very severe (n=2), severe (n=4), moderate (n=11), mild (n=9), and non-anemic (n=12). The severity of anemia was classified based on hematocrit (HCT) values as follows: very severe (<15%), severe (15–20%), moderate (21–25%), mild (26–30%), and non-anemic (>30%). The healthy control group consisted of 9 dogs, matched by age with the infected group. It is important to note that the number of dogs in the very severely anemic group was limited to n=2 due to case availability in the clinical population. Therefore, findings from this subgroup should be interpreted with caution and are intended to be descriptive rather than inferential.

The inclusion and exclusion criteria for animals were as follows:

  1. Age between 1–8 years, avoiding immature or geriatric dogs;
  2. Presence of clinical and pathological signs of CME, including lethargy, anorexia, fever, epistaxis, dyspnea, and splenomegaly;
  3. No comorbidities such as hyperadrenocorticism, diabetes mellitus, hypothyroidism, or other protozoal diseases;
  4. Not pregnant or lactating;
  5. No history of chronic liver damage, congestive heart failure, or treatment with antihypertensive drugs;
  6. Absence of pericardial effusion, congenital heart disease, or moderate to severe mitral or aortic valve insufficiency.

Healthy dogs were over one year of age, vaccinated, regularly treated for internal and external parasites, clinically healthy, and free from CME or other protozoal infections. All dogs included in the infected group tested positive for E. canis antibodies using the SNAP® 4Dx® ELISA test (IDEXX Laboratories, USA), which also screens for Anaplasma spp., Borrelia burgdorferi, and Dirofilaria immitis. Dogs that tested positive for any of these co-infections were excluded from the study. Additionally, SNAP Leishmania tests (IDEXX Laboratories, USA) were performed, and animals with positive results were excluded. In cases where clinical presentation was ambiguous, indirect fluorescent antibody test (IFAT) was applied to confirm E. canis monoinfection with a cut-off titer of 1:50.

All procedures involving animals were conducted in accordance with national and institutional guidelines for the care and use of animals and were approved by the Local Ethics Committee for Animal Experiments (ADU-HADYEK), Aydın Adnan Menderes University, under approval number 64583101/2022/76. This study was supported by the Scientific Research Projects Coordination Unit of Aydın Adnan Menderes University under project code VTF-23023.

 <italic>Sample collection and laboratory analyses</italic>

Blood samples were collected from the cephalic vein (V. cephalica antebrachii) of dogs presented to the Internal Medicine Clinic of Aydın Adnan Menderes University Veterinary Faculty. A total of 6 mL of blood was collected for analysis in separate tubes containing lithium heparin (4 mL) and EDTA (2 mL). Hematological analyses were performed using the Abacus Vet5 (Diatron®, Hungary), and anemia severity was determined. The remaining blood samples were centrifuged at 3,000 rpm for 15 min to obtain plasma samples, which were stored at -20 °C until cTnI levels were measured. Complete blood count was performed using the Abacus Vet5 (Hungary) device. Plasma samples were obtained by centrifugation of lithium heparin tubes at 3,000 rpm for 15 min. Biochemical analyses, including total iron, iron-binding capacity, and transferrin saturation, were conducted using enzymatic colorimetric methods with a Randox autoanalyzer (Randox Laboratories Ltd., UK). cTnI concentrations, a biomarker of myocardial injury, were measured using a fluorescent immunoassay device (Healvet Fia-3000, Healvet Medtech GZ Ltd.®, China). Test kits were stored according to the manufacturer's instructions, and testing procedures were performed within one hour of opening.

 <italic>Echocardiographic evaluation</italic>

Following inclusion in the study, echocardiographic evaluations were conducted using a 5–7 MHz probe, performed by a single operator without the need for sedation to ensure consistency and minimize stress-related physiological variations. Imaging was acquired in both the long and short axes using B-mode and M-mode modalities, facilitating comprehensive assessment of ejection fraction, fractional shortening, and left ventricular dimensions during both systole and diastole. Additionally, mitral valve regurgitation was evaluated. Identical echocardiographic protocols were applied to the healthy control group to ensure comparability.

 <italic>Statistical analysis</italic>

Descriptive statistics were performed for numerical variables, with normality assessed using standard diagnostic tests. The normality of data distribution was evaluated using Shapiro-Wilk tests. Depending on the distribution and homogeneity of variances, statistical comparisons between groups were performed using either one-way ANOVA followed by Tukey’s post-hoc test or the non-parametric Kruskal-Wallis test followed by Dunn’s test for multiple comparisons. Relationships between variables were evaluated using Pearson correlation coefficients. All statistical analyses were conducted using IBM SPSS Statistics for Windows, Version 26.0 (IBM Corp., Armonk, NY, USA), and the significance level was set at p<0.05.

 RESULTS <italic>Clinical findings</italic>

The clinical evaluation of dogs presumptively diagnosed with CME revealed that cough was present in 9.1% of moderately anemic and 8.3% of severely anemic animals. Lethargy was absent in the control group but was observed in 25%, 77.8%, 81.8%, and 100% of non-anemic, mildly anemic, moderately anemic, and severely anemic dogs, respectively. Lymphadenopathy and bleeding manifestations, ranging from petechiae to epistaxis, were particularly notable in dogs with severe anemia. Dermatologic lesions, primarily characterized by exfoliative dermatitis and lunate formations, were prevalent in infected dogs, with a higher frequency among those with moderate to severe anemia, recorded at 63.6% and 75%, respectively. Poor body condition was noted exclusively in dogs with severe anemia.

 <italic>Hematological findings</italic>

Hematological analyses revealed significant differences in anemia severity between the infected groups. Dogs in the very severe and severe anemia groups exhibited markedly lower hematocrit, hemoglobin, and red blood cell (RBC) counts compared to those in the mild and non-anemic groups (p<0.05). White blood cell (WBC) counts were elevated in the infected groups relative to the control group, suggesting an inflammatory response. Platelet counts were significantly reduced in infected dogs, particularly in those with severe anemia (p<0.01) (Table 1).

Haematological findings in Ehrlichiosis-infected and healthy animals
Type of anemia Mild Moderate Severe Very severe Non-anemic Control
WBC (109/L) 9.39±1.93 10.57±2.19 7.17±0.95 0.79±0.27 10.95±1.62 11.27±1.35
NEU (109/L) 6.15±1.38 7.83±1.74 3.05±1.30b 0.51±0.14b 7.99±1.30a 7.63±0.83a
LYM (109/L) 2.27±0.85 1.84±0.49 3.96±1.92 0.25±0.16 3.90±1.92 3.02±0.49
MON (109/L) 0.69±0.23b 1.21±0.55b 0.12±0.03a 0.02±0.01a 1.05±0.29b 0.51±0.08
EOS (109/L) 0.28±0.07 0.23±0.09 0.03±0.01 0.01±0.01 0.21±0.07 0.10±0.06
RBC (1012/L) 5.14±0.10 4.09±0.20b 2.45±0.25b 1.25±0.17b 6.68±0.34a 8.52±0.51a
HGB (g/dL) 23.83±12.28 8.40±0.46b 5.22±0.88b 2.45±0.25b 15.00±0.65a 18.26±0.72a
HCT (%) 33.70±0.64 25.52±0.96b 17.11±1.34b 9.25±1.09b 45.37±2.43a 57.18±1.97a
MCV (fL) 65.77±1.31 56.06±4.56 70.62±2.54 73.50±1.50 67.25±1.42 67.86±3.02
MCH (pg) 22.60±0.83 20.75±1.22 21.15±1.62 19.75±0.65 22.00±0.74 21.79±1.35
MCHC (g/dL) 34.39±1.03 32.91±1.38 30.20±2.97 26.75±0.25 33.05±0.99 31.91±0.64
PLT (109/L) 116.22±45.30 95.67±35.31b 38.25±22.62b 2.00±1.00b 161.44±63.09 300.86±60.6a
MPV (fL) 10.45±1.65 11.89±1.05 6.46±1.87 10.10±1.50 32.41±22.52 9.01±0.29
PCT (%) 0.43±0.19 0.39±0.15 0.06±0.03 0.00±0.00 1.10±0.54 0.27±0.06

a,bValues expressed with different letters in the same row are statistically significantly different (p<0.05).

WBC: white blood cell count; NEU: neutrophil count; LYM: lymphocyte count; MON: monocyte count; EOS: eosinophil count; RBC: red blood cell count; HGB: hemoglobin concentration; HCT: hematocrit; MCV: mean corpuscular volume; MCH: mean corpuscular hemoglobin; MCHC: mean corpuscular hemoglobin concentration; PLT: platelet count; MPV: mean platelet volume; PCT: plateletcrit.

 <italic>Biochemical findings</italic>

Biochemical analyses demonstrated that total iron levels and transferrin saturation were significantly lower in dogs with severe anemia compared to non-anemic and control groups (p<0.05). Iron-binding capacity was elevated in the severe anemia group, reflecting altered iron metabolism associated with the infection. Elevated cardiac troponin I (cTnI) levels were observed in infected dogs, with the highest mean concentration detected in the severely anemic group (0.29±0.22 ng/mL), significantly higher than in control animals (0.01±0.00 ng/mL) (p<0.01) (Table 2).

Iron profile and cTnI findings in Ehrlichiosis-infected and healthy animals
Type of anemia Mild Moderate Severe Very severe Non-anemic Control
cTnI (ng/mL) 0.22±0.19 0.29±0.22 0.01±0.00 0.01±0.00 0.17±0.16 0.01±0.00
Iron (µg/dl) 165.44±11.15 178.64±30.09 188.75±48.95 272.50±175.50 162.50±24.75 155.44±12.88
Iron-binding capacity (µg/dl) 282.67±17.81 251.27±16.61 224.50±12.50b 219.50±0.50b 336.33±22.51 345.44±9.62a
Transferrin saturation (%) 62.11±7.72 69.76±10.26 82.07±18.85 123.96±79.67 48.37±6.99 44.74±3.06

a,bValues expressed with different letters in the same row are statistically significantly different (p<0.05).

cTnI: cardiac troponin I; Iron: serum iron concentration.

 <italic>Echocardiographic findings</italic>

Echocardiographic evaluation revealed significant changes in cardiac dimensions and function among infected dogs. Dogs with severe anemia had increased left ventricular end-diastolic and end-systolic dimensions compared to the control group (p<0.05). Fractional shortening and ejection fraction were reduced in the severely anemic group, indicating compromised cardiac function. Mitral valve regurgitation was more prevalent in infected dogs, particularly those with moderate to severe anemia (Table 3).

Echocardiographic findings in Ehrlichiosis-infected and healthy animals
Type of anemia Mild Moderate Severe Very severe Non-anemic Control
IVSd (cm) 0.98±0.66 1.16±0.60b 1.10±0.07 1.07±0.05 1.00±0.36 0.89±0.60a
LVIDd (cm) 3.19±0.44 3.27±0.19 3.56±0.54 3.13±0.25 3.71±0.20 2.81±0.13
LVPWd (cm) 0.80±0.95 0.99±0.08 1.12±0.23 0.84±0.22 0.86±0.06 0.75±0.08
EDV (ml) 40.37±15.45 42.67±6.31 60.19±18.31 39.34±7.68 50.20±5.89 30.61±3.49
IVSs (cm) 1.00±0.11 1.38±0.10b 1.52±0.16 1.41±0.11 0.98±0.08a 1.12±0.08
LVIDs (cm) 1.94±0.28 1.93±0.17 1.71±0.22 1.89±0.42 2.15±0.15 1.62±0.12
LVPWs (cm) 1.03±0.14 1.38±0.08 1.62±0.11 1.21±0.42b 1.14±0.74 0.95±0.09a
ESV (ml) 14.41±5.02 13.20±2.50 9.37±2.49 12.07±6.34 17.64±3.08 8.48±0.81
SV (ml) 25.95±11.13 29.56±4.27 55.21±14.94 27.27±1.33 35.01±4.55 20.79±3.56
EF (%) 65.60±3.84 67.32±3.99 77.58±8.19 71.35±10.55 63.81±3.10 68.39±2.62
FS (%) 33.89±2.75a 38.41±2.40 53.83±7.71b 40.35±8.65 36.91±3.17 37.67±2.30
LA/Ao 1.19±0.04 1.27±0.11 1.26±0.15 1.30±0.24 1.36±0.13 1.21±0.04

a,bValues expressed with different letters in the same row are significantly different (p<0.05).

IVSd: interventricular septal thickness in diastole; LVIDd: left ventricular internal diameter in diastole; LVPWd: left ventricular posterior wall thickness in diastole; EDV: end-diastolic volume; IVSs: interventricular septal thickness in systole; LVIDs: left ventricular internal diameter in systole; LVPWs: left ventricular posterior wall thickness in systole; ESV: end-systolic volume; SV: stroke volume; EF: ejection fraction; FS: fractional shortening; LA/Ao: left atrium-to-aortic root ratio.

 <italic>Correlation analysis</italic>

Pearson correlation analysis revealed a significant negative correlation between hematocrit levels and cTnI concentrations (r=-0.62, p<0.01). Additionally, left ventricular dimensions were positively correlated with cTnI levels (r=0.55, p<0.05) (Table 4).

Correlation of hematocrit levels and echocardiographic parameters
Echocardiographic parameters HCT (%) IVSd (cm) LVIDd (cm) LVPWd (cm) EDV (mL) IVSs (cm) LVIDs (cm) LVPWs (cm) ESV (mL) SV (mL) EF (%) FS (%) LA_Ao
HCT (%) Pearson Correlation 1 -0.308 0.145 -0.223 0.028 -0.472** 0.191 -0.290 0.199 -0.065 -0.207 -0.259 -0.033
P value 0.060 0.385 0.178 0.865 0.003 0.250 0.077 0.230 0.697 0.212 0.117 0.844
IVSd (cm) Pearson Correlation -0.308 1 -0.014 0.515** -0.103 0.357* -0.072 0.358* -0.108 -0.070 0.000 -0.107 -0.004
P value 0.060 0.933 0.001 0.539 0.028 0.666 0.027 0.517 0.678 0.998 0.523 0.979
LVIDd (cm) Pearson Correlation 0.145 -0.014 1 -0.009 0.859** 0.217 0.715** 0.094 0.683** 0.766** -0.193 0.062 0.242
P value 0.385 0.933 0.958 0.000 0.190 0.000 0.573 0.000 0.000 0.246 0.712 0.144
LVPWd (cm) Pearson Correlation -0.223 0.515** -0.009 1 0.052 0.341* -0.064 0.691** -0.033 0.083 0.100 0.073 0.150
P value 0.178 0.001 0.958 0.757 0.036 0.701 0.000 0.846 0.622 0.550 0.662 0.368
EDV (mL) Pearson Correlation 0.028 -0.103 0.859** 0.052 1 0.385* 0.754** 0.222 0.747** 0.935** -0.180 0.112 0.164
P value 0.865 0.539 0.000 0.757 0.017 0.000 0.180 0.000 0.000 0.280 0.503 0.326
IVSs (cm) Pearson Correlation -0.472** 0.357* 0.217 0.341* 0.385* 1 0.012 0.551** -0.002 0.494** 0.180 0.423** 0.222
P value 0.003 0.028 0.190 0.036 0.017 0.945 0.000 0.990 0.002 0.279 0.008 0.181
LVIDs (cm) Pearson Correlation 0.191 -0.072 0.715** -0.064 0.754** 0.012 1 -0.040 0.966** 0.523** -0.604** -0.515** 0.012
P value 0.250 0.666 0.000 0.701 0.000 0.945 0.810 0.000 0.001 0.000 0.001 0.943
LVPWs (cm) Pearson Correlation -0.290 0.358* 0.094 0.691** 0.222 0.551** -0.040 1 -0.049 0.307 0.219 0.326* 0.154
P value 0.077 0.027 0.573 0.000 0.180 0.000 0.810 0.771 0.061 0.186 0.046 0.356
ESV (mL) Pearson Correlation 0.199 -0.108 0.683** -0.033 0.747** -0.002 0.966** -0.049 1 0.514** -0.555** -0.487** -0.014
P value 0.230 0.517 0.000 0.846 0.000 0.990 0.000 0.771 0.001 0.000 0.002 0.933
SV (mL) Pearson Correlation -0.065 -0.070 0.766** 0.083 0.935** 0.494** 0.523** 0.307 0.514** 1 0.033 0.374* 0.216
P value 0.697 0.678 0.000 0.622 0.000 0.002 0.001 0.061 0.001 0.842 0.021 0.193
EF (%) Pearson Correlation -0.207 0.000 -0.193 0.100 -0.180 0.180 -0.604** 0.219 -0.555** 0.033 1 0.688** 0.175
P value 0.212 0.998 0.246 0.550 0.280 0.279 0.000 0.186 0.000 0.842 0.000 0.293
FS (%) Pearson Correlation -0.259 -0.107 0.062 0.073 0.112 0.423** -0.515** 0.326* -0.487** 0.374* 0.688** 1 0.162
P value 0.117 0.523 0.712 0.662 0.503 0.008 0.001 0.046 0.002 0.021 0.000 0.331
LA_Ao Pearson Correlation -0.033 -0.004 0.242 0.150 0.164 0.222 0.012 0.154 -0.014 0.216 0.175 0.162 1
P value 0.844 0.979 0.144 0.368 0.326 0.181 0.943 0.356 0.933 0.193 0.293 0.331

**p<0.01 *p<0.05 WBC: white blood cell count; NEU: neutrophil count; LYM: lymphocyte count; MON: monocyte count; EOS: eosinophil count; BAS: basophil count; RBC: red blood cell count; HGB: hemoglobin concentration; HCT: hematocrit; MCV: mean corpuscular volume; MCH: mean corpuscular hemoglobin; MCHC: mean corpuscular hemoglobin concentration; RDWCV: red cell distribution width-coefficient of variation; RDWSD: red cell distribution width-standard deviation; PLT: platelet count; MPV: mean platelet volume; PDW: platelet distribution width; PCT: platelet crit; BUN: blood urea nitrogen; CREA: creatinine; NA: sodium; K: potassium; CL: chloride; SDMA: symmetric dimethylarginine; PROBNP: N-terminal pro-brain natriuretic peptide; VHS: vertebral heart size; CAKG: heart size on X-ray; T: temperature; PDK: right atrial diameter; RDK: right ventricular diameter; KDSSN: interatrial septum thickness; IVSd: interventricular septal thickness in diastole; LVIDd: left ventricular internal diameter in diastole; LVPWd: left ventricular posterior wall thickness in diastole; IVSs: interventricular septal thickness in systole; LVIDs: left ventricular internal diameter in systole; LVPWs: left ventricular posterior wall thickness in systole; FS: fractional shortening; EF: ejection fraction; Ao: aortic root diameter

DISCUSSION

Canine Monocytic Ehrlichiosis (CME) is a globally distributed disease caused by the gram-negative, obligate intracellular bacterium E. canis, primarily transmitted by the brown dog tick, Rhipicephalus sanguineus (12, 13, 14). Transmission could also occur through blood transfusion from an infected donor 15. The incubation period of E. canis in dogs ranges from 8 to 20 days 16. Typical clinical manifestations include high body temperature, ophthalmic lesions, epistaxis, and lymphadenopathy 17. Other reported clinical signs include lethargy, depression, anorexia, hemorrhage, neurological symptoms, ocular inflammation, chronic weight loss, hind limb edema, pallor of mucous membranes, and cachexia 17. The clinical findings in this study were consistent with previously reported data, with lethargy, lymphadenopathy, and exfoliative dermatitis being the most frequent signs, particularly in severely anemic cases.

Anemia in CME can be attributed to several mechanisms, with mild to moderate anemia commonly observed during the acute phase as a result of inflammation-related processes. Chronic-phase anemia is typically characterized as normochromic, normocytic, and non-regenerative due to extensive bone marrow involvement 18. Previous studies indicate that anemia severity escalates as the disease progresses to its chronic stage, evidenced by declining hematocrit levels 19, 20. In this study, anemia presence and severity were determined based on positive E. canis antibody detection via rapid test kits, which precluded differentiation of acute, chronic, or exposure phases, as well as antigen detection by PCR.

Hematocrit values were significantly lower in moderately, severely, and very severely anemic dogs compared to the non-anemic group, suggesting chronic phase involvement or prolonged exposure. Consistent with Kaewmongkol et al. (2017), platelet counts in infected dogs exhibited significant reduction in moderately to severely anemic groups compared to non-anemic or healthy controls 19. Thrombocytopenia is a hallmark hematologic alteration in CME, particularly in the acute and chronic phases, characterized by significant platelet depletion, while subclinical cases often exhibit no platelet changes 21. Pancytopenia development during the chronic phase, associated with bone marrow suppression, further underscores the chronic nature of the disease in these cases. Our findings demonstrated a parallel between changes in hematocrit and platelet counts, with significantly lower platelet counts observed in moderately, severely, and very severely anemic dogs compared to non-anemic and healthy controls. These thrombocytopenic profiles align with previous reports emphasizing the role of thrombocytopenia in CME pathogenesis, underscoring its significance in diagnosing ehrlichiosis 21, 22, 23.

Total leukocyte counts exhibited a decline from the non-anemic to the very severely anemic groups, although no significant differences were noted between healthy and infected groups. Neutrophil counts were significantly reduced in the severely and very severely anemic groups compared to the non-anemic and healthy controls, in agreement with Dixit et al. 24. However, De Castro et al. 25 reported contrasting results, with significantly elevated neutrophil levels in infected dogs compared to healthy controls, possibly attributed to underlying co-infections.

Iron metabolism is pivotal in the pathophysiology of CME, with approximately 60–70% of total body iron contained in hemoglobin 26, 27. Disruptions in iron absorption, utilization, or loss can result in conditions such as iron deficiency anemia or anemia due to inflammation 28. In this study, serum iron, iron-binding capacity, and transferrin saturation were assessed across different anemia severity groups. No significant differences were found among the groups, likely due to the chronic or indolent nature of the disease, which allows iron stores to be maintained at adequate levels.

Myocardial injury is a recognized sequela of CME, frequently indicated by elevated cardiac troponin I (cTnI) levels, implying multisystemic involvement 29. Previous studies have reported myocardial damage in naturally infected dogs, reflected by increased cTnI levels 2, 30. In our study, elevated cTnI levels were observed in only a limited number of cases, suggesting that myocardial injury was not pervasive among the examined animals. The stage of infection—acute or chronic—may influence cTnI elevation, as acute myocardial injury can be detected within four hours of ischemic events 31. This could briefly explain our results obtained in this study.

Echocardiographic evaluation in the current study revealed that interventricular septal thickness during diastole (IVSd) was significantly greater in moderately anemic dogs compared to the healthy group, suggesting a compensatory adaptation to anemia. This increased thickness was also observed during systole (IVSs) in moderately anemic dogs compared to non-anemic infected and healthy dogs. Additionally, the fractional shortening (FS) percentage was higher in the severely anemic group compared to the mildly anemic group. These findings imply that myocardial changes are associated with anemia severity and may represent ongoing compensatory mechanisms in response to chronic anemia 32, 33. Consistent with previous studies, the echocardiographic parameters indicated hyperdynamic cardiac function, characterized by increased systolic performance while maintaining diastolic function 32, 33.

In conclusion, our findings demonstrate that anemia severity was closely correlated with both myocardial and hematological alterations in E. canis-infected dogs. The observed echocardiographic changes further support the presence of compensatory mechanisms in response to chronic anemia. Although the number of animals in the very severely anemic group was limited, their inclusion still provided valuable observational insight into extreme clinical cases. Future studies employing controlled infection models and larger sample sizes are essential to better understand the progression of myocardial injury and the influence of co-infections in ehrlichiosis.

 CONCLUSION

Differences were observed in several cardiac and hematological parameters depending on the severity of anemia. No significant differences in cardiac troponin I (cTnI) levels were found, but echocardiography revealed higher interventricular septal thickness during diastole (IVSd) and systole (IVSs) in moderately anemic dogs, suggesting early compensatory remodeling. Fractional shortening was greater in severely anemic dogs, indicating enhanced cardiac function as a compensatory response. Chronic anemia may induce cardiac changes that could progress to dysfunction, emphasizing the need for early intervention. E. canis-infected dogs may present with cardiac abnormalities such as left ventricular hypertrophy. Comprehensive studies using advanced diagnostics, including cardiac MRI and Doppler echocardiography, are needed to better understand disease progression.

 CONFLICT OF INTEREST

The authors declare that they have no financial or non-financial conflict of interest regarding authorship and publication of this article.

 ACKNOWLEDGMENTS

This study is a summary of a section from the Master's thesis entitled "Investigation of Echocardiographic and Hematological Changes in Naturally Infected Dogs With Ehrlichiosis" conducted at the Department of Internal Medicine, Institute of Health Sciences, Aydın Adnan Menderes University. It was supported by Aydın Adnan Menderes University Scientific Research Projects Unit under project number VTF-23023.

 AUTHORS’ CONTRIBUTION

All authors made the conceptualization and methodology of the study. HE and ZU were responsible for the project administration. ZU, TÖ, SE and HE conducted the investigation and data curation. ZE, KU and HE performed the formal analysis. All authors were included in the writing of the original draft, review and editing of the manuscript.

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