ORIGINAL_ARTICLE Physico-chemical and microbiological characteristics, sensory quality and acceptability of native chicken and rabbit sausage produced with corn oil, margarine and beef fat In this paper, we evaluated the effect of certain oils or fats (corn oil, margarine and beef fat) on the physico-chemical, microbiological characteristics and sensory quality of native chicken and rabbit sausages as low fat sausages. The sausages were evaluated by physicochemical analysis of pH, aw, moisture content, protein and fat, TPC and Salmonella as microbiological characteristics, as well as flavor, juiciness and overall acceptability as sensory quality, and with regards to diversification of meat products in Indonesia. Three formulations for native chicken (FA) and rabbit (FB) sausages were prepared: FA1 and FB1 (with 10% corn oil); FA2 and FB2 (with 10% margarine): FA3 and FB3 (with 10% beef fat). Results indicated no significant difference (p<0.05) between the pH and aw values of native chicken and rabbit sausages using different fat sources. The moisture content was significantly different (p<0.05) both between the native chicken and rabbit sausages and the types of fat source (corn oil, margarine and beef fat) added. No effect on protein content was found both in native chicken and rabbit sausages. The lowest fat content was found in rabbit sausages (18.79 + 0.70%) where corn oil was added as a fat source. Lowest TPC was also observed. Salmonella were not detected in any of the sausage groups. There was no significant difference (p<0.05) in various sensory attributes. In conclusion, native chicken and rabbit meat seems to be a suitable alternative for low fat sausages production, particularly with corn oil as fat source. https://macvetrev.mk/Files/Article/2019/10.1515/macvetrev-2016-0087/macvetrev-2016-0087.pdf 2016-10-15T09:00:00 193 199 10.2478/macvetrev-2016-0087 native chicken sausages rabbit sausages corn oil margarine beef fat Hendronoto Arnoldus Walewangko Lengkey lengkeyhendronoto@gmail.com false 1 Food Technology Department, Animal Husbandry, Universitas Padjadjaran, Indonesia LEAD_AUTHOR Balia Roostita Lobo false 2 Food Technology Department, Animal Husbandry, Universitas Padjadjaran, Indonesia AUTHOR Buckle K.A, Buckle K.A, Edwards R.A, Fleet G.H, Wootton M, Meat science and technologyFood Science 1978; Australian Vice-Chancellor’s Committee; 143-167. 1 Muir W.M, Aggrey S. E, Poultry genetics, breeding and biotechnology 2003; Wallingford. UK: CABI Publishing; 58- 2 McNitt J.I, Patton N.M, Lukefahr S.D, Cheeke P.R, Rabbit production 2011; 8th ed. Wallingford, UK: Paperback CABI; 453-475. 3 Olivares A, Navarro J.L, Salvador A, Flores M, Sensory acceptability of slow fermented sausages based on fat content and ripening timeMeat Sci 2010; 86: 251-257. PMid: 20510528 4 10.1016/j.meatsci.2010.04.005 Koutsopoulos D.A, Koutsimanis G.E, Blougas J.G, Effect of carrageenan level and packaging during ripening on processing and quality characteristics of low-fat fermented sausages produced with olive-oilMeat Sci 2008; 79: 1188-197. PMid: 22062612 5 10.1016/j.meatsci.2007.08.016 Ospina E.J.C, Cruz-S A, Perez-Alvarez J.A, Fernandez-Lopez J, Development of Combinations of chemically modified oils as pork backfat substitutes in sausages formulationsMeat Sci 2010; 84: 3491-497. PMid: 20374815 6 10.1016/j.meatsci.2009.10.003 Lengkey H.A.W, Suryaningsih L, Sembor S, Balia R.L, The effect of various fat on rabbit sausages chemical properties. Prosiding Seminar Nasional Peternakan Berkelanjutan 6. November, 18-192014; Bandung, Indonesia: Fakultas Peternakan Universitas Padjadjaran; 310-315.in Indonesian 7 Jimenez-Colmenero F, Triki M, Herrero A.M, Rodriguez-Salas L, Healthy oil combination stabilized in a konjac matrix as pork fat replacement in low-fat, PUFA-enriched, dry fermented sausagesLWT-Food Science and Technology 2013; 51: 158-163. 8 10.1016/j.lwt.2012.10.016 Menegas L.Z, Pimentel T.C, Gracia S, Prudencio S.H, Dry-fermented chicken sausage produced with inulin and corn oil: Physico-chemical, microbiological, and textural characteristics and acceptability during storageMeat Sci 2013; 93: 501-506. PMid: 23273457 9 10.1016/j.meatsci.2012.11.003 Rossi L.P.R, Almeida R.C.C, Lopes L.S, Figueiredo A.C.L, Ramos M.P.P, Almeida P.F, Occurrence of Listeria spp. in Brazilian fresh sausage and control of Listeria monocytogenes using bacteriophage P100Food Control 2010; 22: 954-958. 10 10.1016/j.foodcont.2010.12.001 AOAC. Official methods of analysis 1990; 15th ed. Arlington, Virginia, USA: Association of official analytical chemists; 931-948. 11 Maturin L, Peeler J.T, Bacteriological analytical manual. Chapter 3. Aerobic plate count 2001; Available at: http://www.fda.gov/Food/FoodScienceResearch/LaboratoryMethods/ucm063346. 12 Heinz G, Hautzinger P, Meat processing technology 2007; Bangkok: FAO Regional office for Asia and the Pacific; 335- 13 Larmond E, Laboratory methods for sensory evaluation of food 1977; Research branch Canada department of agriculture. Publication 11637; 57- 14 Cocolin L, Rantsiou K, Iacumin L, Urso R, Cantoni C, Comi G, Study of the ecology of fresh sausages and characterization of population of lactic acid bacteria by molecular methodJ.Appl. Environ. Microbiol 2004; 70: 41883-1894. PMCid: PMC383013 15 10.1128/AEM.70.4.1883-1894.2004 Fernando-Lopez J, Sendra E, Sayas-Barbera E, Navarro C, Perez-Alvarez J.A, Physico-chemical and microbiological profiles of salchichon (Spanish dry-fermented sausage) enriched with orange fibreEuropean Food Research and Technology 2008; 221: 410-417. 16 Thomas R, Anjaneyulu A.S.R, Kondaiah N, Quality of hurdle treated pork sausages during refrigerated (4 +10C) storageJ Food Sci Technol 2010; 47: 3266-272. PMid: 23572635 PMCid: PMC3551018 17 10.1007/s13197-010-0041-1 International Commission on Microbiological Specifications for Food. Microorganisms in foods 2002; 6: Gaithersburg. Maryland: Microbial ecology of food commodities. Aspen Publ. Inc; 18 Wijtes T, Mc Clure P.J, Zwietering M.H, Roberts T.A, Modeling bacterial growth of Listeria monocytogenes as a function of water activity, pH and temperatureInt. J. Food Microbiol 1993; 18: 139-149. 19 10.1016/0168-1605(93)90218-6 Delgado-Pando G, Cofrades S, Ruiz-Capillas C, Jimenez-Colmenero F, Healthier lipid combination oil-in water emulsions prepared with various protein systems: an approach for development of functional meat productsEuropean Journal of Lipid Science and Technology 2010; 112: 7791-801. 20 10.1002/ejlt.200900234 Nistor E, Bampidis V.A, Pacala N, Pentea M, Tozer J, Prundeanu H, Nutrient content of rabbit meat as compared to chicken, beef and pork meatJ. Anim. Prod. Adv 2013; 3: 172-176. 21 10.5455/japa.20130411110313 Baiao N.C, Lara L.J.C, Oil and fat in broiler nutritionBrazil J. Poult. Sci 2005; 7: 129-141. 22 10.1590/s1516-635x2005000300001