Original Scientific Article
Fatty acid composition of ostrich (Struthio camelus) abdominal adipose tissue
Daniela Belichovska * ,
Zehra Hajrulai-Musliu ,
Risto Uzunov ,
Katerina Belichovska ,
Mila Arapcheska

Mac Vet Rev 2015; 38 (1): 53 - 59

10.14432/j.macvetrev.2014.11.028

Received: 04 July 2014

Received in revised form: 30 October 2014

Accepted: 05 November 2014

Available Online First: 20 November 2014

Published on: 15 March 2015

Correspondence: Daniela Belichovska,
PDF HTML

Abstract

Fatty acid composition of foods has a great impact on nutrition and health. Therefore, the determination and knowledge of the fatty acid composition of food is very important for nutrition. Due to the high nutritional characteristics of ostrich meat and its products, the research determining their quality is of topical interest. The aim of the present investigation was the determination of fatty acid composition of ostrich adipose tissue. The content of fatty acids was determined according to AOAC Official Methods of Analysis and determination was performed using a gas chromatograph with a flame-ionization detector (GC-FID). The results are expressed as a percentage of the total content of fatty acids. The method was validated and whereupon the following parameters were determined: linearity, precision, recovery, limit of detection and limit of quantification. The repeatability was within of 0.99 to 2.15%, reproducibility from 2.01 to 4.57%, while recovery ranged from 94.89 to 101.03%. According to these results, this method is accurate and precise and can be used for analysis of fatty acids in foods. It was concluded that the content of saturated fatty acids (SFA) accounted 34.75%, of monounsaturated fatty acids (MUFA) 38.37%, of polyunsaturated fatty acids (PUFA) 26.88%, of total unsaturated fatty acids (UFA) 65.25% and of desirable fatty acids (DFA) (total unsaturated + stearic acid) 70.37% of the analysed samples. The ratio polyunsaturated/saturated fatty acids accounted 0.77. The most present fatty acid is the oleic (C18:1n9c) with 28.31%, followed by palmitic (C16:0) with 27.12% and linoleic (C18:2n6c) acid with 25.08%. Other fatty acids are contained in significantly lower quantities.

Keywords: ostrich, adipose tissue, fatty acids, validation, GC-FID


References

1. AOAC. Official Methods of Analysis of AOAC International 2005; 18th Edition. Gaithersburg, MD, USA:
2. Basuny A.M.M, Arafat S.M, Nasef S.L, Utilization of ostrich oil in foodsInt. Res. J. Biochem. Bioinfor 2011; 2: 199-208.
3. Escobar S, Processing of the fat in commercial oil of ostrich 2003; http://www.world-ostrich.org/download/ostoilen.pdf.
4. Frontczak M, Krysztofiak K, Bilska A, Uchman W, Characteristics of fat from African ostrich Struthio camelusFood Sci. Technol 2008; 11: 420-428.
5. Grompone A.M, Irigaray B, Gil M, Uruguayan nandu (Rhea americana) oil:A comparison with emu and ostrich oilsJ. Am. Oil Chem. Soc 2005; 82: 687-689.
http://dx.doi.org/10.1007/s11746-005-1130-1.
6. Harris S.D, Morris C.A, May S.G, Jackson T.C, Lucia L.M, Hale D.S, Miller R.K, Keeton J.T, Savell J.W, Acuff G.R, Ostrich Meat Industry DevelopmentFinal report to AOA. Texas Agricultural Extension Service, the Texas A&M University System, College Station, TX, USA 1994;
7. Hoffman L.C, Joubert M, Brand T.S, Manley M, The effect of dietary fish oil rich in n – 3 fatty acids on the organoleptic, fatty acid and physicochemical characteristics of ostrich meatMeat Sci 2005; 70: 45-53. http://dx.doi.org/10.1016/j.meatsci.2004.11.019. PMid:22063279
8. Hoffman L.C, Brand M.M, Cloete S.W.P, Muller M, The fatty acid composition of muscles and fat depots of ostriches as influenced by genotypeS. Afr. J. Anim. Sci 2012; 42: 256-265. http://dx.doi.org/10.4314/sajas.v42i3.7.
9. Horbañczuk J.O, Cooper R.G, Jóźwik A, Klewiec J, Krzyżewski J, Malecki I, Chyliński W, Wójcik A, Kawka M, Cholesterol content and fatty acid composition of fat from culled breeding ostriches (Struthio camelus)Anim. Sci. Pap. Rep 2003; 21: 271-275.
10. Horbañczuk J.O, Malecki I, Cooper R.G, Jóźwik A, Klewiec J, Krzyżewski J, Khalifa H, Chyliński W, Wójcik A, Kawka M, Cholesterol content and fatty acid composition of two fat depots from slaughter ostriches (Struthio camelus) aged 14 monthsAnim. Sci. Pap. Rep 2004; 22: 247-251.
11. Morris C.A, Harris S.D, May S.G, Jackson T.C, Hale D.S, Miller R.K, Keeton J.T, Acuff G.R, Lucia L.M, Savell J.W, Ostrich slaughter and fabrication:1. Slaughter yields of carcasses and effects of electrical stimulation on post-mortem pHPoult. Sci 1995; 74: 1683-1687. http://dx.doi.org/10.3382/ps.0741683. PMid:8559734
12. Poławska E, Jóźwik A, Wójcik A, Strzałkowska N, Pierzchała M, Tolik D, Półtorak A, Hoffman L.C, Effect of dietary linseed and rapeseed supplementation on fatty acids profiles in the ostriches. Part 2Fat. Anim. Sci. Pap. Rep 2013; 31: 347-354.
13. Pollok K.D, Hale D.S, Miller R.K, Angel R, Blue-McLendon A, Baltmanis B, Keeton J.T, Ostrich slaughter and by-product yieldsAmerican Ostrich 1997; 4: 31-35.
14. Rhee K. S, Chow C.K, Fatty acids in meat and meat productsIn:Fatty acids in foods and their health implications 1992; New York: Marcel Dekker, Inc; 65-93.
15. Rulebook for specific requirements for food of animal origin, Official Gazette of RM no. 115/2008
16. Sales J, Franken L, Ostrich fatAust. Ostrich Assoc. J 1996; 37: 39-45.
17. Sales J, Marais D, Kruger M, Fat Content, caloric value, cholesterol content and fatty acid composition of row and cooked ostrich meatJ. Food Compos. Anal 1996; 9: 85-89. http://dx.doi.org/10.1006/jfca.1996.0010.
18. Sales J, Horba-czuk J.O, Dingle J, Coleman R, Sensik S, Carcass characteristics of emus (Dromaius novaehollandiae)Br. Poult. Sci 1999; 40: 145-147.
http://dx.doi.org/10.1080/00071669987999. PMid:10405052
19. Shahryar H.A, Lotfi A, Fatty acid composition of fat depot in 11 month old slaughtered ostriches, Struthio camelus LCurrent Biotica 2012; 6: 246-250.
20. Taverniers I, De Loose M, Van Bockstaele E, Trends in quality in the analytical laboratory:Analytical method validation and quality assuranceTrends in analytical chemistry 2004; 23: 535-552. http://dx.doi.org/10.1016/j.trac.2004.04.001.
21. WHO/FAO. Diet Nutrition and the Prevention of Chronic Diseases 2003; Geneve: WHO; 4-101.(cit. Poławska et al., 2013)
22. Stein J, Kulemeier J, Lembcke B, Caspary W.F, Simple and rapid method for determination of short-chain fatty acids in biological materials by high-performance liquid chromatography with ultraviolet detectionJ Chromatogr 1992; 576: 153-61. http://dx.doi.org/10.1016/0378-4347(92)80174-O.


Copyright

© 2015 Belichovska D. This is an open-access article published under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Conflict of Interest Statement

The authors declared that they have no potential conflict of interest with respect to the authorship and/or publication of this article.

Citation Information

Macedonian Veterinary Review. Volume 38, Issue 1, Pages 53-59, p-ISSN 1409-7621, e-ISSN 1857-7415, DOI: 10.14432/j.macvetrev.2014.11.028, 2015