Original Scientific Article
Preliminary investigation of the possibility for implementation of modified pharmacopoeial HPLC methods for quality control of metronidazole and ciprofloxacin in medicinal products used in veterinary medicine
Marjan Piponski
*
,
Tanja Bakovska
,
Marina Naumoska
,
Tatjana Rusevska
,
Gordana Trendovska Serafimovska
,
Hristina Andonoska
Received: 03 June 2014
Received in revised form: 25 September 2014
Accepted: 10 November 2014
Available Online First: 17 November 2014
Published on: 15 March 2015
Correspondence: Marjan Piponski, piponski@yahoo.com
Abstract
Quality control of veterinary medicine products containing two different frequently used antibiotics metronidazole and ciprofloxacin hydrochloride, was considered and performed, using modified pharmacopoeial HPLC methods. Three different HPLC systems were used: Varian ProStar, Perkin Elmer Series and UPLC Shimadzu Prominence XR. The chromatographic columns used were LiChropher RP Select B 75 mm x 4 mm with 5 µm particles and Discovery C18 100 mm x 4,6 mm with 5 µm particles. Chromatographic methods used for both analytes were compendial, with minor modifications made for experimental purposes. Minor modifications of the pharmacopoeia prescribed chromatographic conditions, in both cases, led to better chromatographic parameters, good resolution and shorter analysis times. Optimized methods can be used for: determination of metronidazole in gel formulation, for its simultaneous quantification with preservatives present in the formulation and even for identification and quantification of its specified impurity, 2-methyl-5-nitroimidazole; determination of ciprofloxacin hydrochloride in film coated tablets and eye drops and identification and quantification of its specified impurities. These slightly modified and optimized pharmacopoeial methods for quality control of metronidazole and ciprofloxacin dosage forms used in veterinary medicine can be successfully applied in laboratories for quality control of veterinary medicines.
Keywords: metronidazole, ciprofloxacin hydrochloride, veterinary medicines, pharmacopoeia, quality control
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Copyright
© 2015 Piponski M. This is an open-access article published under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Conflict of Interest Statement
The authors declared that they have no potential conflict of interest with respect to the authorship and/or publication of this article.
Citation Information
Macedonian Veterinary Review. Volume 38, Issue 1, Pages 31-42, p-ISSN 1409-7621, e-ISSN 1857-7415, DOI: 10.14432/j.macvetrev.2014.11.029, 2015
10.14432/j.macvetrev.2014.11.029
