Original Scientific Article
Asssessment of the effect of selected components of equine seminal plasma on semen freezability
Miroslava Mráčková * ,
Marta Zavadilová ,
Markéta Sedlinská

Mac Vet Rev 2015; 38 (1): 91 - 96

10.14432/j.macvetrev.2015.01.037

Received: 09 September 2014

Received in revised form: 02 January 2015

Accepted: 16 January 2015

Available Online First: 19 January 2015

Published on: 15 March 2015

Correspondence: Miroslava Mráčková, mrackovam@vfu.cz
PDF HTML

Abstract

In this study, selected components of seminal plasma in equine semen were evaluated. Levels of enzymes, electrolytes, microelements and some other components were observed. The aim of this study was to find some important differences between the levels of these components and the total sperm motility after freezing and thawing (freezability of the semen). Total of 32 ejaculates from 7 stallions were collected, assessed and prepared in 0,5 ml straws for freezing. After thawing, the sperm motility was analyzed and ejaculates were divided into two groups: “good” freezable and “poor” freezable. The only statistically significant difference between groups of „good” and „poor” freezable ejaculates was in the concentration of vitamin E in the seminal plasma. In the group of „good” freezable ejaculates, the level of vitamin E was significantly lower (p≤0,05) than in the group of “poor” freezable ejaculates.

Keywords: stallion, seminal plasma, semen freezing, vitamin E


References

1. Kareskoski M, Katila T, Components of stallion seminal plasma and the effect of seminal plasma on sperm longevityAnim Reprod Sci 2008; 107: 249-256.
http://dx.doi.org/10.1016/j.anireprosci.2008.04.013. PMid:18556156
2. Barrier-Battut I, Delajarraud H, Legrand E, Bruyas J-F, Fieni F, Tainturier D, Thorin C, Pouliquen H, Calcium, magnesium, cooper, and zinc in seminal plasma of fertile stallions, and their relationship with semen freezabilityTheriogenology 2002; 58: 229-232. http://dx.doi.org/10.1016/S0093-691X(02)00744-6.
3. Brinsko S P, Blanchard T.L, Varner D.D, Schumacher J, Love C.C, Hinrichs K, Hartmen D, Manual of equine reproduction 2011; 3rd ed. Missouri: Mosby; 19-192.
4. Trein C.R, Zikler H, Bustamante-Filho I.C, Malschitzky E, Jobim M.I.M, Sieme H, Mattos R.C, Equine seminal plasma proteins related with semen freezabilityAnim Reprod Sci 2008; 107: 252-253. http://dx.doi.org/10.1016/j.anireprosci.2008.05.129.
5. Jasko D.L, Moran D.L, Farlin M.E, Squires E.L, Effect of seminal plasma dilution or removal on spermatozoa motion characteristics of cooled semenTheriogenology 1991; 35: 1059-1067. http://dx.doi.org/10.1016/0093-691X(91)90354-G.
6. Braun J, Sakai M, Hochi S, Oguri N, Preservation of ejaculated and epididymal stallion spermatozoa by cooling and freezingTheriogenology 1994; 41: 809-818.
http://dx.doi.org/10.1016/0093-691X(94)90497-7.
7. Alghamdi A.S, Troedsson M.H, Xue J.L, Crabo B.G, Effect of seminal plasma concentration and various extenders on post-thaw motility and glass wool - Sephadex filtration of cryopreserved stallion semenAm J Vet Res 2002; 63: 880-885. http://dx.doi.org/10.2460/ajvr.2002.63.880. PMid:12061536
8. Ben W.X, Fu M.T, Mao L.K, Ming Z.W, Xiong W.W, Effects of various concentration of native seminal plasma in cryoprotectant on viability on human spermArch Androl 1997; 39: 211-216. http://dx.doi.org/10.3109/01485019708987918. PMid:9352032
9. Aurich J.E, Kuhne A, Hoope H, Aurich C, Seminal plasma effects membrane integrity and motility of equine spermatoza after cryopreservationTheriogenology 1996; 46: 791-797. http://dx.doi.org/10.1016/S0093-691X(96)00237-3.
10. Katila T, Anderson M, Reilas T, Koskinen E, Post-thaw motility and viability of fractionated and frozen stallion ejaculatesTheriogenology 2002; 58: 241-244.
http://dx.doi.org/10.1016/S0093-691X(02)00783-5.
11. Morrell J.M, Pihl J, Dalin A.M, Johannisson A, Restoration of seminal plasma to stallion spermatozoa selected by colloid centrifugation increase progressive motility but is determinal to chromatin integrityTheriogenology 2012; 78: 345-352. http://dx.doi.org/10.1016/j.theriogenology.2012.02.009. PMid:22494676
12. Aurich J.E, Kuhne A, Hoope H, Aurich C, Effect of seminal plasma on stallion semen quality after cryopreservationJ Reprod Fertil Abstr Ser 1998; 15: 34-
13. Ackay E, Reilas T, Andersson M, Katila T, Effect of seminal plasma fractions on sperm survival after cooled storageJ Vet Med A 2006; 53: 481-485.
http://dx.doi.org/10.1111/j.1439-0442.2006.00882.x. PMid:17054486
14. Taylor K, Roberts P, Sanders K, Burton P, Effect of antioxidant supplementation of cryopreservation medium on post-thaw integrity of human spermatozoaRBM online 2009; 18: 184-189.
15. Pech-Sansores A.G.C, Centurion-Castro F.G, Rodriguez-Buenfil J.C, Segura-Correa J.C, Ake-Lopez J.R, Effect of the addition of seminal plasma, vitamin E and incubation time on post-thawed sperm viability in boar semenTropical and Subtropical Agroecosystems 2011; 14: 965-971.
16. Therond P, Auger J, Legrand A, Jouannet P, α-tocopherol in human spermatozoa and seminal plasma:relationship with mitility, antioxidant enzymes and leukocytesMolecular Human Reproduction 1996; 10: 739-744. http://dx.doi.org/10.1093/molehr/2.10.739.
17. Mahmoud G.B, Abdel-Raheem S.M, Hussein H.A, Effect of combination of vitamin E and selenium injections on reproductive performance and blood parameters of Ossimi ramsSmall Ruminant Res 2013; 113: 103-108. http://dx.doi.org/10.1016/j.smallrumres.2012.12.006.
18. Contri A, De Amicis I, Molinari A, Faustini M, Gramenzi A, Robbe D, Carluccio A, Effect of dietary antioxidant supplementation on fresh semen quality in stallionTheriogenology 2011; 75: 1319-1326. http://dx.doi.org/10.1016/j.theriogenology.2010.12.003. PMid:21295825
19. Deichsel K, Palm F, Koblischke P, Budik S, Aurich C, Effect of a dietary antioxidant supplementation on semen quality in pony stallionsTheriogenology 2008; 69: 940-945. http://dx.doi.org/10.1016/j.theriogenology.2008.01.007. PMid:18358523
20. Franco J.S.V, Chaveiro A, Góis A, da Silva F.M, Effects of α-tocopherol and ascorbic acid on equine semen quality after cryopreservationJ Eq Vet Sci 2013; 33: 787-793.
http://dx.doi.org/10.1016/j.jevs.2012.12.012.
21. Schmid-Lausigk Y, Aurich C, Influences of a diet supplemented with linseed oil and antioxidants on quality of equine semen after cooling and cryopreservation during winterTheriogenology 2014; 81: 966-973. http://dx.doi.org/10.1016/j.theriogenology.2014.01.021. PMid:24576708
22. Blomfield J, Tucker L, McLeay L, Morris L.H.A, Evaluation of antioxidant dietary supplementation on semen quality parameters in New Zealand Standard bred stallionsJ Eq Vet Sci 2014; 34: 89- http://dx.doi.org/10.1016/j.jevs.2013.10.061.
23. Bertelsmann H, Keppler S, Holtershinken M, Bollwein H, Behne D, Alber D, Bukalis G, Kyriakopoulos A, Sieme H, Selenium in blood, semen, seminal plasma and spermatozoa of stallions and its relationship to sperm qualityReprod Fertil Dev 2010; 22: 886-891. http://dx.doi.org/10.1071/RD10032. PMid:20450841
24. Fuse H, Kazama T, Ohta S, Fujiuchi Y, Relationship between zinc concentrations in seminal plasma and variuos sperm parametersInternational Urology and Nephrology 1999; 31: 401-408. http://dx.doi.org/10.1023/A:1007190506587.
25. Colagar A.H, Marzony E.T, Chaichi M.J, Zinc levels in seminal plasma are associated with sperm quality in fertile and infertile menNutrition Research 2009; 29: 82-88.
http://dx.doi.org/10.1016/j.nutres.2008.11.007.
26. Wong W.Y, Filk G, Groenen P.M, Swinkels D.W, Thomas C.M, Copius-Peereboom J.H, Merkus H.M, Steegers-Theunissen R.P, The impact of calcium, magnesium, zinc, and cooper in blood and seminal plasma on semen parameters in menReprod Toxicol 2001; 15: 131-136. http://dx.doi.org/10.1016/S0890-6238(01)00113-7.
27. Amann R.P, Pickett B.W, Principles of cryopreservation and a review of cryopreservation of stallion spermatozoaJ Eq Vet Sci 1987; 7: 145-173.
http://dx.doi.org/10.1016/S0737-0806(87)80025-4.
28. Kareskoski M, Reilas T, Andersson M, Katila T, Motility and plasma membrane integrity of spermatozoa in fractionated stallion ejaculates after storageReprod Dom Anim 2006; 41: 33-38. http://dx.doi.org/10.1111/j.1439-0531.2006.00647.x. PMid:16420325
29. Pesch S, Bergmann M, Bostedt H, Determination of some enzymes and macro- and microelements in stallion seminal plasma and their correlations to semen qualityTheriogenology 2006; 66: 307-313. http://dx.doi.org/10.1016/j.theriogenology.2005.11.015. PMid:16413936
30. Kareskoski M, Reilas T, Sankari S, Andersson M, Guvenc K, Katila T, Alkaline and acid phosphatase, β-glucuronidase and electrolyte levels in fractionated stallion ejaculatesReprod Dom Anim 2010; 45: 396-374. http://dx.doi.org/10.1111/j.1439-0531.2009.01579.x. PMid:20074319
31. Koskinen E, Karlsson M, Reilas T, Sankari S, Esala A-L, Katila T, Catalase activity and total protein in fractionated stallion seminal plasmaTheriogenology 2002; 58: 337-340. http://dx.doi.org/10.1016/S0093-691X(02)00767-7.
32. Kohdaira T, Kinoshita Y, Konno M, Oshima H, Distribution of G-glutamyl-transpeptidase in male reproductive system of rats and its age-related changesAndrologia 1986; 18: 610-617. http://dx.doi.org/10.1111/j.1439-0272.1986.tb01839.x. PMid:2880528
33. Hinton B.T, Lan Z.J, Rudolph D.B, Labus J.C, Lye R.J, Testicular regulation of epididymal gene expressionJ Reprod Fertil, Suppl 1998; 53: 47-58.
34. Kutzler M.A, Solter P.F, Hoffmann W.E, Volkmann D.H, Characterization and localization of alkaline phosphatase in canine seminal plasma and gonadal tissuesTheriogenology 2003; 60: 299-306. http://dx.doi.org/10.1016/S0093-691X(02)01366-3.
35. Turner R.M.O, McDonnell S, Alkaline phosphatase in stallion semen:chracterization and clinical applicationsTheriogenology 2003; 60: 1-10.
http://dx.doi.org/10.1016/S0093-691X(02)00956-1.
36. Kosiniak-Kamysz K, Bittmar A, Podstawski Z, Alkalina phosphatase activity as a marker of dog semen freezabilityZootehniesi Biotehnologii 2007; 40: 131-135.


Copyright

© 2015 Mráčková M. This is an open-access article published under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Acknowledgment

This project was supported by grant no. IGA 59/2013/FVL.

Conflict of Interest Statement

The authors declared that they have no potential conflict of interest with respect to the authorship and/or publication of this article.

Citation Information

Macedonian Veterinary Review. Volume 38, Issue 1, Pages 91-96, p-ISSN 1409-7621, e-ISSN 1857-7415, DOI: 10.14432/j.macvetrev.2014.10.024, 2015