Original Scientific Article
Asssessment of the effect of selected components of equine seminal plasma on semen freezability
Miroslava Mráčková
*
,
Marta Zavadilová
,
Markéta Sedlinská
Received: 09 September 2014
Received in revised form: 02 January 2015
Accepted: 16 January 2015
Available Online First: 19 January 2015
Published on: 15 March 2015
Correspondence: Miroslava Mráčková, mrackovam@vfu.cz
Abstract
In this study, selected components of seminal plasma in equine semen were evaluated. Levels of enzymes, electrolytes, microelements and some other components were observed. The aim of this study was to find some important differences between the levels of these components and the total sperm motility after freezing and thawing (freezability of the semen). Total of 32 ejaculates from 7 stallions were collected, assessed and prepared in 0,5 ml straws for freezing. After thawing, the sperm motility was analyzed and ejaculates were divided into two groups: “good” freezable and “poor” freezable. The only statistically significant difference between groups of „good” and „poor” freezable ejaculates was in the concentration of vitamin E in the seminal plasma. In the group of „good” freezable ejaculates, the level of vitamin E was significantly lower (p≤0,05) than in the group of “poor” freezable ejaculates.
Keywords: stallion, seminal plasma, semen freezing, vitamin E
References
2. Barrier-Battut I, Delajarraud H, Legrand E, Bruyas J-F, Fieni F, Tainturier D, Thorin C, Pouliquen H, Calcium, magnesium, cooper, and zinc in seminal plasma of fertile stallions, and their relationship with semen freezabilityTheriogenology 2002; 58: 229-232. http://dx.doi.org/10.1016/S0093-691X(02)00744-6.
3. Brinsko S P, Blanchard T.L, Varner D.D, Schumacher J, Love C.C, Hinrichs K, Hartmen D, Manual of equine reproduction 2011; 3rd ed. Missouri: Mosby; 19-192.
5. Jasko D.L, Moran D.L, Farlin M.E, Squires E.L, Effect of seminal plasma dilution or removal on spermatozoa motion characteristics of cooled semenTheriogenology 1991; 35: 1059-1067. http://dx.doi.org/10.1016/0093-691X(91)90354-G.
7. Alghamdi A.S, Troedsson M.H, Xue J.L, Crabo B.G, Effect of seminal plasma concentration and various extenders on post-thaw motility and glass wool - Sephadex filtration of cryopreserved stallion semenAm J Vet Res 2002; 63: 880-885. http://dx.doi.org/10.2460/ajvr.2002.63.880. PMid:12061536
8. Ben W.X, Fu M.T, Mao L.K, Ming Z.W, Xiong W.W, Effects of various concentration of native seminal plasma in cryoprotectant on viability on human spermArch Androl 1997; 39: 211-216. http://dx.doi.org/10.3109/01485019708987918. PMid:9352032
11. Morrell J.M, Pihl J, Dalin A.M, Johannisson A, Restoration of seminal plasma to stallion spermatozoa selected by colloid centrifugation increase progressive motility but is determinal to chromatin integrityTheriogenology 2012; 78: 345-352. http://dx.doi.org/10.1016/j.theriogenology.2012.02.009. PMid:22494676
12. Aurich J.E, Kuhne A, Hoope H, Aurich C, Effect of seminal plasma on stallion semen quality after cryopreservationJ Reprod Fertil Abstr Ser 1998; 15: 34-
14. Taylor K, Roberts P, Sanders K, Burton P, Effect of antioxidant supplementation of cryopreservation medium on post-thaw integrity of human spermatozoaRBM online 2009; 18: 184-189.
15. Pech-Sansores A.G.C, Centurion-Castro F.G, Rodriguez-Buenfil J.C, Segura-Correa J.C, Ake-Lopez J.R, Effect of the addition of seminal plasma, vitamin E and incubation time on post-thawed sperm viability in boar semenTropical and Subtropical Agroecosystems 2011; 14: 965-971.
16. Therond P, Auger J, Legrand A, Jouannet P, α-tocopherol in human spermatozoa and seminal plasma:relationship with mitility, antioxidant enzymes and leukocytesMolecular Human Reproduction 1996; 10: 739-744. http://dx.doi.org/10.1093/molehr/2.10.739.
17. Mahmoud G.B, Abdel-Raheem S.M, Hussein H.A, Effect of combination of vitamin E and selenium injections on reproductive performance and blood parameters of Ossimi ramsSmall Ruminant Res 2013; 113: 103-108. http://dx.doi.org/10.1016/j.smallrumres.2012.12.006.
18. Contri A, De Amicis I, Molinari A, Faustini M, Gramenzi A, Robbe D, Carluccio A, Effect of dietary antioxidant supplementation on fresh semen quality in stallionTheriogenology 2011; 75: 1319-1326. http://dx.doi.org/10.1016/j.theriogenology.2010.12.003. PMid:21295825
21. Schmid-Lausigk Y, Aurich C, Influences of a diet supplemented with linseed oil and antioxidants on quality of equine semen after cooling and cryopreservation during winterTheriogenology 2014; 81: 966-973. http://dx.doi.org/10.1016/j.theriogenology.2014.01.021. PMid:24576708
22. Blomfield J, Tucker L, McLeay L, Morris L.H.A, Evaluation of antioxidant dietary supplementation on semen quality parameters in New Zealand Standard bred stallionsJ Eq Vet Sci 2014; 34: 89- http://dx.doi.org/10.1016/j.jevs.2013.10.061.
23. Bertelsmann H, Keppler S, Holtershinken M, Bollwein H, Behne D, Alber D, Bukalis G, Kyriakopoulos A, Sieme H, Selenium in blood, semen, seminal plasma and spermatozoa of stallions and its relationship to sperm qualityReprod Fertil Dev 2010; 22: 886-891. http://dx.doi.org/10.1071/RD10032. PMid:20450841
24. Fuse H, Kazama T, Ohta S, Fujiuchi Y, Relationship between zinc concentrations in seminal plasma and variuos sperm parametersInternational Urology and Nephrology 1999; 31: 401-408. http://dx.doi.org/10.1023/A:1007190506587.
26. Wong W.Y, Filk G, Groenen P.M, Swinkels D.W, Thomas C.M, Copius-Peereboom J.H, Merkus H.M, Steegers-Theunissen R.P, The impact of calcium, magnesium, zinc, and cooper in blood and seminal plasma on semen parameters in menReprod Toxicol 2001; 15: 131-136. http://dx.doi.org/10.1016/S0890-6238(01)00113-7.
28. Kareskoski M, Reilas T, Andersson M, Katila T, Motility and plasma membrane integrity of spermatozoa in fractionated stallion ejaculates after storageReprod Dom Anim 2006; 41: 33-38. http://dx.doi.org/10.1111/j.1439-0531.2006.00647.x. PMid:16420325
30. Kareskoski M, Reilas T, Sankari S, Andersson M, Guvenc K, Katila T, Alkaline and acid phosphatase, β-glucuronidase and electrolyte levels in fractionated stallion ejaculatesReprod Dom Anim 2010; 45: 396-374. http://dx.doi.org/10.1111/j.1439-0531.2009.01579.x. PMid:20074319
33. Hinton B.T, Lan Z.J, Rudolph D.B, Labus J.C, Lye R.J, Testicular regulation of epididymal gene expressionJ Reprod Fertil, Suppl 1998; 53: 47-58.
34. Kutzler M.A, Solter P.F, Hoffmann W.E, Volkmann D.H, Characterization and localization of alkaline phosphatase in canine seminal plasma and gonadal tissuesTheriogenology 2003; 60: 299-306. http://dx.doi.org/10.1016/S0093-691X(02)01366-3.
36. Kosiniak-Kamysz K, Bittmar A, Podstawski Z, Alkalina phosphatase activity as a marker of dog semen freezabilityZootehniesi Biotehnologii 2007; 40: 131-135.
Copyright
© 2015 Mráčková M. This is an open-access article published under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Acknowledgment
This project was supported by grant no. IGA 59/2013/FVL.
Conflict of Interest Statement
The authors declared that they have no potential conflict of interest with respect to the authorship and/or publication of this article.
Citation Information
Macedonian Veterinary Review. Volume 38, Issue 1, Pages 91-96, p-ISSN 1409-7621, e-ISSN 1857-7415, DOI: 10.14432/j.macvetrev.2014.10.024, 2015
10.14432/j.macvetrev.2015.01.037
