Original Scientific Article
The prevalence of Pasteurella multocida from farm pigs in Serbia
Oliver Radanovic
*
,
Jadranka Zutic
,
Dobrila Jakic-Dimic
,
Branislav Kureljusic
,
Bozidar Savic
Received: 20 July 2015
Received in revised form: 16 October 2015
Accepted: 19 October 2015
Available Online First: 13 November 2015
Published on: 15 March 2016
Correspondence: Oliver Radanovic, radanovic.oliver@gmail.com
Abstract
The investigations covered a total of 234 lungs from necropsied pigs with different pneumonic lesions, from 6 farrow-to-finish pig farms during 2013 and 2014. The samples were inoculated on selective culture media and aerobically incubated at 37oC and in carbon dioxide condition. The isolated bacterial colonies were further characterised morphologically and biochemically. The identification was confirmed using the BBL Crystal, E/N, G/P ID Kit (Becton Dickinson). For determination of the type of Pasteurella multocida, the PCR method was used. The findings showed that bacteria were isolated from 202 (86%) out of 234 examined lung samples. The pure isolates of Pasteurella multocida were obtained from 71 (35 %) samples. Out of the remaining 29 (14%) examined lung samples, 9, 8, 7 and 5 examined lung samples were shown as mixed cultures of Pasteurella multocida and Streptococcus spp., Arcanobacterium pyogenes, Actinobacillus pleuropneumoniae and Haemophilus parasuis, respectively. The PCR method confirmed that all 15 investigated strains of P. multocida belong to type A.
Keywords: Pasteurella multocida, lung, pig
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Copyright
© 2015 Radanovic O. This is an open-access article published under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Conflict of Interest Statement
The authors declared that they have no potential conflict of interest with respect to the authorship and/or publication of this article.
Citation Information
Macedonian Veterinary Review. Volume 39, Issue 1, Pages 39-42, p-ISSN 1409-7621, e-ISSN 1857-7415, DOI: 10.1515/macvetrev-2015-0063, 2016
10.1515/macvetrev-2015-0063
