Original Scientific Article
Anthelmintic resistance and associated management practices in local horses in Sokoto metropolis, Nigeria
Abubakar Musa Mayaki * ,
Fatimah Folashade Mohammed ,
Sherifat Banke Idris

Mac Vet Rev 2018; 41 (1): 55 - 64

10.1515/macvetrev-2017-0031

Received: 30 March 2017

Received in revised form: 21 September 2017

Accepted: 15 November 2017

Available Online First: 14 December 2017

Published on: 15 March 2018

Correspondence: Abubakar Musa Mayaki, bubakar241@yahoo.com
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Abstract

This study was carried out to assess the management practices used in the control of gastrointestinal (GI) nematodes of horses and to determine the efficacy of three anthelmintics commonly used in Sokoto metropolis. A questionnaire was administered on management practices, while an anthelmintic efficacy test was carried out using 15 horses. The 15 horses were divided into three groups (A, B and C) comprising of 5 each and treated with albendazole, ivermectin and fenbendazole, respectively. The faecal egg count reduction test (FECRT) was used to determine the efficacy and faecal culture was used to determine the parasite species. Majority of the respondents (80%) claimed to have worm control strategies, but only 32.5% used anthelmintics for the control of GI parasites. 62.5% of respondents designed their deworming plan, while only 25% relied on veterinarians. Most of the treatments were done by the horse owners and/or handlers and they largely depended on visual judgement in dosage determination. Their selection of anthelmintics was based on familiarity and 52.5% of the respondents dewormed their horses six times a year using a particular class of anthelmintic or herbal remedies. Resistance against albendazole as well as suspected resistance against fenbendazole by the GI nematodes identified was observed, while ivermectin demonstrated high efficacy against all nematodes isolated. In conclusion, a single dose of subcutaneous injection of ivermectin was highly effective against gastrointestinal parasites in horses, while the worm control strategies employed by respondents enhanced the selection of nematode resistance to albendazole and fenbendazole.

Keywords: anthelmintic efficacy, horses, gastrointestinal parasites, Sokoto

References

1. Mayaki, A.M., Ibrahim, J.I. (2015). Prevalence of equine stereotypic behaviour and its associated risk factors in Kano Metropolis, Nigeria. Afr J Livest Ext. 15, 54-60.
2. Mahfooz, A., Masood, M.Z., Yousaf, A., Akhtar, N., Zafar-Pakistan, M.A. (2008). Prevalence and anthelmintic efficacy of Abamectin, against gastrointestinal parasites of horses. Pakistan Vet J. 28(2):76-78.
3. Relf, V.E., Morgan, E.R., Hodgkinson, J.E., Matthews, J.B. (2013). Helminth excretion with regard to age, gender and management practices on UK Thoroughbred studs. Parasitology 140, 641–652. https://doi.org/10.1017/S0031182012001941 PMid:23351718
4. Morariu, S., Mederle, N., Badea, C., Dărăbuş, G., Ferrari, N., Genchi, C. (2016). The prevalence, abundance and distribution of cyathostomins (small strongyles) in horses from western Romania. Vet Parasitol. 223, 205-209. https://doi.org/10.1016/j.vetpar.2016.04.021 PMid:27198801
5. Laugier, C., Sevin, C., Ménard, S., Maillard, K. (2012). Prevalence of Parascaris equorum infection in foals on French stud farms and first report of ivermectin-resistant P. equorum populations in France. Vet Parasitol. 188, 185–189. https://doi.org/10.1016/j.vetpar.2012.02.022 PMid:22494940
6. Reinemeyer, C.R. (2012). Anthelmintic resistance in non-strongylid parasites of horses. Vet Parasitol. 185, 9–15. https://doi.org/10.1016/j.vetpar.2011.10.009 PMid:2207∬
7. Traversa, D., Castagna, G., von Samson-Himmelstjerna, G., Meloni, S., Bartolini, R., Geurden, T., Pearce, M.C., Woringer, E., Besognet, B., Milillo, P., D'Espois, M. (2012). Efficacy of major anthelmintics against horse cyathostomins in France. Vet Parasitol. 188, 294–300. https://doi.org/10.1016/j.vetpar.2012.03.048 PMid:22538094
8. von Samson-Himmelstjerna, G. (2012). Anthelmintic resistance in equine parasites:detection, potential clinical relevance and implications for control. Vet Parasitol. 185, 2–8. https://doi.org/10.1016/j.vetpar.2011.10.010 PMid:22100141
9. Peregrine, A.S., Molento, M.B., Kaplan, R.M., Nielsen, M.K. (2014). Anthelmintic resistance in important parasites of horses:does it really matter?Vet Parasitol. 201, 1–8. https://doi.org/10.1016/j.vetpar.2014.01.004
10. Gilleard, J.S., Beech, R.N. (2007). Population genetics of anthelmintic resistance in parasitic nematodes. Parasitology134, 1133-1147. https://doi.org/10.1017/S0031182007000066 PMid:17608973
11. Kaplan, R.M., Nielsen, M.K. (2010). An evidence-based approach to equine parasite control:it ain't the 60s anymore. Equine Vet Educ. 22, 306–16. https://doi.org/10.1111/j.2042-3292.2010.00084.|ax
12. Alayande, M.O., Onakpa, M.M., Lawal, M.D. (2003). Prevalence of helminth parasites in horses in Sokoto. Nig J Parasitol. 24, 191-193.
13. Useh, N.M., Oladele, S.B., Ibrahim, N.D., Nok, A.J., Esievo, K.A. (2005). Prevalence of equine diseases in the northern Guinea Savannah of Zaria, Nigeria. J Equine Sci. 16(1):27-28. https://doi.org/10.1294/jes.16.27
14. Wosu, M., Udobi, S.O. (2014). Prevalence of gastrointestinal helminths of horses (Equuscaballus) in the southern Guinea Savannah Zone of Northern, Nigeria. J Vet Adv. 4(4):499-502.
15. Sugun, M.Y., Bresibe, F., Mailafiya, S., Muhammad, M., Suleiman, I., Abechi, A.S. (2015). Prevalence of gastro-intestinal parasite in Donkeys in and around Maiduguri metropolis, Borno state, Nigeria. Direct Res J Agric Food Sci. 3(4):70-73.
16. Fashanu, S.O., Fagbemi, B.O. (2003). A preliminary investigation of resistance to anthelmintics in strongyles of cattle in Shaki, Nigeria. Afr J Biomed Res. 6, 111 –112.
17. Wikipedia. (2015). [Internet]. https://en.m.wikipidia.org/wiki/sokoto_state
18. FAOSTAT (2013). FAO statistic databases. [Internet]. Food and agriculture organization of the united nations, rome. Italy. www.faostat.fao.org/site/573/default.aspx
19. Canadian Council on Animal Care Guide (CCAC) (1993). http://www.ccac.ca/Documents/Standards/Guidelines/Experimental_Animals_Vol1.pdf , retrieved 04/1/2016.
20. Santos, R.F. (1981). Estimation of genetic and phenotypic parameters of the body of horses. The Cava of Brazilian saddle and other Equidae. (pp. 288). Botucatu, Publisher.
21. MAFF. (1986). Ministry of Agriculture, Fisheries and Food. Manual of Veterinary parasitological laboratory techniques. (pp. 160). 3rd ed. Ref. Book 418, H.M.S.O. London, UK.
22. Cernea, M., Madeira de Carvalho, L.M., V. Cozma. (2008). Identification of third stage strongyle larvae (L3). In:Atlas of Diagnosis of Equine Strongylidosis. (pp. 128). Editura Academic Pres.
23. Coles, G.C., Bauer, C., Borgsteede, F.H., Geerts, S., Klei, T.R., Taylor, M.A., Waller, P.J. (1992). World Association for the Advancement of Veterinary Parasitology (W.A.A.V.P.) methods for the detection of anthelmintic resistance in nematodes of veterinary importance. Vet Parasitol. 44, 35–44. https://doi.org/10.1016/0304-4017(92)90141-U
24. Agaie, B.M., Onyeyili, P.A. (2007). Anthelmintic activity of the crude aqueous leaf extracts of Anogeissus leiocarpus in sheep. Afr J Biotechnol. 6(13):1511-1515.
25. Suleiman, M.M., Mamman, M., Sidiama, A., Ighoja, E.J., Tauheed, M.,Talba, A.M. (2014). Evaluation of anthelmintic activity of Nigerian ethnoveterinary plants;Cassia occidentalis and Guiera senegalensi. Veterinary World 7(7):536-541. https://doi.org/10.14202/vetworld.2014.536-541
26. Veerakumari, L., Priya, P. (2006). In vitro effect of Azadirachtin on the motility and acetycholinestrease activity of Cotylophoron cotyphorum (Fischoeder, 1901). J Vet Parasitol, 20, 1–5.
27. Qiao, J., Zou, X., Lai, D., Yan, Y., Wang, Q., Li, W., Deng, S., Xu, H., Gu, H. (2013). Azadirachtin blocks the calcium channel and modulates the cholinergic miniature synaptic current in the central nervous system of Drosophila. Pest. Manag. Sci. 70(7):1041-1047. https://doi.org/10.1002/ps.3644 PMid:24002996
28. Kaplan, R.M. (2002). Anthelmintic resistance in nematodes of horses. Vet Res. 33, 491-507. https://doi.org/10.1051/vetres:2002035 PMid:123∬6
29. Relf, V.E., Morgan, E.R., Hodgkinson, J.E., Matthews, J.B. (2012). A questionnaire study on parasite control practices on UK breeding Thoroughbred studs. Equine Vet J. 44, 466-471. https://doi.org/10.1111/j.2042-3306.2011.00493.|ax PMid:22050130
30. Cabaret, J., Benoit, M., Laignel, G., Nicourt, C. (2009). Current management of farms and internal parasites by conventional and organic meat sheep French farmers and acceptance of targeted selective treatments. Vet Parasitol. 164, 21–29. https://doi.org/10.1016/j.vetpar.2009.04.018 PMid:19414221
31. Kenyon, F., Jackson, F. (2012). Targeted flock/herd and individual ruminant treatment approaches. Vet Parasitol. 186 (1-2):10–17. https://doi.org/10.1016/j.vetpar.2011.11.041 PMid:22188982
32. Fritzen, B., Rohn, K., Schnieder, T., von Samson-Himmelstjerna, G. (2010). Endoparasite control management on horse farms –lessons from worm prevalence and questionnaire data. Equine Vet J. 42, 79-83. https://doi.org/10.2746/042516409X471485 PMid:20121919
33. Volk, J.O., Felsted, K.E., Thomas, J.G., Siren, C.W. (2011). Executive summary of the Bayer veterinary care usage study. J Am Vet Med Assoc. 238(10):1275–1282. https://doi.org/10.2460/javma.238.10.1275 PMid:2156≄
34. Chapman, M.R., French, D.D., Klei, T.R. (2002). Gastrointestinal helminths of ponies in Lousiana:a comparison of species currently prevalent with those present 20 years ago. J Parasitol. 88(6):1130-1134. https://doi.org/10.1645/0022-3395(2002)088[1130:GHOPIL]2.0.|aCO;2
35. Umar, Y.A., Maikaje, D.B., Garba, U.M., Alhassan, M.A.F. (2013). Prevalence of gastrointestinal parasites of horses used for cadet training in Nigeria. J. Vet. Adv. 3(2):43-48. https://doi.org/10.5455/jva.20130219032443
36. Biu, A.A., Olatunde, K.T., Konto, M., Paul, B.T., Ya'uba, A.M. (2012). Prevalence and quantitative faecal analysis of equine Gastrointestinal nematodosis in Maiduguri, Nigeria. Journal Medical and Applied Biosciences 4, 1-6.
37. Kuzmina, T.A., Dzeverin, I., Kharchenko, V.A. (2016). Strongylids in domestic horses:Influence of horse age, breed and deworming programs on the strongyle parasite community. Vet Parasitol. 227, 56–63. https://doi.org/10.1016/j.vetpar.2016.07.024 PMid:27523938
38. Santos, D.W., Dias de Castro, L.L., Giese, E.G., Molento, M.B. (2016). Morphometric study of infective larvae of cyathostomins of horses and their distribution. J Equ Vet Sci. 44, 49–53. https://doi.org/10.1016/j.jevs.2016.02.237
39. Ali, M. (2005). Prevalence and treatment trials against Gastrodiscus infestation and its effect on various blood components in horses. M.Sc. thesis, (Dep. of Clinical Medicine Surgery), Univ. Vet. Anim. Sci. Lahore, Pakistan.
40. Mahboob, K., Khan, J.A., Khan, M.S. (2008). Prevalence, chemotherapy and haematology of strongylosis in horses of district layyah. J. Anim. Plant. Sci., 18(4):117–119.
41. Yadav, K. (2013). Efficacy of different doses of Neem (Azadirachtaindica) against gastroinstestinal nematodes in horses. M.V.Sc. &A.H. thesis (Dep. of Veterinary Medicine), Nanaji Deshmukh Veterinary Science University, Jabalpur.
42. Belmonte, C., Fernandes, F., Gaspary, J., Gama, J., Dotto, F., Otto, M.A., Monteiro, S.G. (2009). Antiparasitics in the control of helmintoses in horses kept in a native field in the central region of Rio Grande do Sul, Brazil. Veterinary in Focus 7(1):46-51.
43. Relf, V.E., Lester, H.E., Morgan, E.R., Hodgkinson, J.E., Mathews, J.B. (2014). Anthelmintic resistance on UK thorough breed stud farms. Int J Parasitol. 44, 507-514. https://doi.org/10.1016/j.ijpara.2014.03.006 PMid:24746779
44. Stratford, C.H., Lester, H.E., Pickles, K.J., McGorum, B.C., Mathews, J.B. (2014). An investigation of anthelmintic efficacy against strongyles on equine yards in Scotland. Equine Vet J. 46, 17–24. https://doi.org/10.1111/evj.12079 PMid:23662803
45. Varady, M., Konigova, A., Corba, J. (2000). Benzimidazole resistance in equine cyathostomins in Slovakia. Vet Parasitol. 94, 67–74. https://doi.org/10.1016/S0304-4017(00)00366-6
46. Slocombe, J.O.D., Coté, J.F., Gannes, R.V. (2008). The persistence of benzimidazole-resistant cyathostomes on horse farms in Ontario over 10 years and the effectiveness of ivermectin and moxidectin against these resistant strains. Cannadian Vet J. 49(1):56–60.
47. Brady, H.A., Nichols, W.T. (2009). Drug resistance in equine parasites:an emerging global problem. J. Equine Vet Sci. 29, 285-295. https://doi.org/10.1016/j.jevs.2009.04.186
48. Traversa, D., von Samson-Himmelstjerna, G., Demeler, J., Milillo, P., Schurmann, S., Barnes, H., Otranto, D., Perrucci, S., di Regalbono, A.F., Beraldo, P., Boeckh, A., Cobb, R. (2009). Anthelmintic resistance in cyathostomin populations from horse yards in Italy, United Kingdom and Germany. Parasite and Vectors2 (Suppl 2) S2. https://doi.org/10.1186/1756-3305-2-S2-S2 PMid:19778463 PMCid:PMC2751838
49. Nielsen, M.K. (2009). Restrictions of anthelmintic usage:perspectives and potential consequences. Parasites and Vectors2 (Suppl 2) S7. https://doi.org/10.1186/1756-3305-2-S2-S7 PMid:19778468 PMCid:PMC2751843

Copyright

©2017 Mayaki A.M. This is an open-access article published under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Acknowledgement

The authors wish to thank Dr. Shehu Sidi and Dr. Abdulrahman Balarabe for helping with horse owner’s contact and Prof. A.O. Talabi for his comments and corrections to the manuscript.

Conflict of Interest Statement 

The authors declared that they have no potential conflict of interest with respect to the authorship and/or publication of this article.

Citation Information

Macedonian Veterinary Review. Volume 41, Issue 1, Pages 55-64, p-ISSN 1409-7621, e-ISSN 1857-7415, DOI:  10.1515/macvetrev-2017-0031, 2018

The all content of the Macedonian Veterinary Review, except where otherwise noted, is licensed under a Creative Commons Attribution License.