Original Scientific Article
Comparison of the chemical composition of whey cheeses: urda and ricotta
Snežana Paskaš,
Jelena Miočinović,
Mila Savić,
Goran Ješić,
Mladen Rašeta,
Zsolt Becskei*

Mac Vet Rev 2019; 42 (2): 151 - 161


Received: 21 September 2018

Received in revised form: 12 July 2019

Accepted: 12 July 2019

Available Online First: 31 August 2019

Published on: 15 October 2019

Correspondence: Zsolt Becskei, beckeizolt@gmail.com


This study was conducted to evaluate the nutritional quality of two types of cheese prepared from bovine whey: Urda and Ricotta. Cheeses were examined for chemical parameters and their amino acids profile was determined. The results revealed that the chemical composition of the observed whey cheeses was highly significantly different (p<0.01) from each other. The amounts of dry matter, fat, protein, lactose, ash and salt were higher in Ricotta compared with Urda cheese samples. On the other hand, Urda contained more moisture, fat on a dry matter basis and moisture on a fat-free basis (79.59%, 27.50%, 84.27% versus 69.82%, 21.02%, 74.56%, respectively). Especially higher yield, salt in moisture content and energy values were observed in Ricotta cheese (5.93%, 3.12%, 145.99Kcal/100g versus 4.39%, 2.40%, 108.97Kcal/100g; Ricotta and Urda, respectively). Whey cheeses are a particularly good source of amino acids containing approximately - Ricotta: leucine (1.60g/100g), lysine (1.17g/100g), phenylalanine (0.78g/100g) and followed by threonine (0.77g/100g), while Urda contained leucine (0.80g/100g), lysine (0.45g/100g), threonine (0.68g/100g) and phenylalanine (0.31g/100g). Both cheese varieties contained glutamic and aspartic acid as the predominant non-essential amino acids (Ricotta: 3.91g/100g and 1.68g/100g versus Urda: 1.65g/100g and 0.80g/100g, respectively). Generally, from a nutritional point of view, the investigated whey cheeses could be considered as cheeses with low salt and fat content, as well as cheeses particularly abundant in branched chain amino acids (leucine, isoleucine and valine). These results also emphasize their advantages as products that present a cost-effective way of dealing with whey as waste material.

Keywords: Urda, Ricotta, chemical composition, amino acids profile, nutritional value


  1. Fox, P.,  Guinee,  P.T.,  Cogan,  M.T., McSweeney, L.H.P. (2000). Fundamentals  of  cheese  science  (pp. 504-513).
  2. Gaithersburg, Maryland: Aspen Publishers Ko, S., Kwak, H.S. (2009). Bioactive components in whey products. In: Park, Y.W., (Ed.), Bioactive components in milk and dairy products (pp. 263-284). Ames, IA, USA: Wiley-Blackwell. https://doi.org/10.1002/9780813821504.ch11 PMid:19132382
  3. Pintado, M.E., Macedo, A.C., Malcata, F.X. (2001). Review: Technology, chemistry and microbiology of whey cheeses. Food Science Technology International 7 (2):105-116. https://doi.org/10.1177/108201301772660277
  4. Addeo, F., Masi, P. (1992). Production of pasta cheese. Cogan T.M. (Ed.), 3rd cheese symposium, National Dairy Products Research Center. Moorepark, (pp. 31-40). Teagasc, Fermoy, Co. Cork. Ireland
  5. Popović Vranješ, A. (2015). Special cheesemaking (pp. 115-401). The University of Novi Sad, Faculty of Agriculture, Department of Animal Science, Indjija: Komazec press. [in Serbian]
  6. Fox, P., McSweeney, L.H.P.,  Cogan,  M.T., Guinee, P.T. (2004). Cheese: chemistry, physics and microbiology. General aspects. 3rd Edition (pp. 978-981). Amsterdam: Elsevier Applied Science.
  7. Davies, A.,   Bevis,   H.E.,   Delves   Broughton,(1997). The use of the bacteriocin, nisin, as a preservative in ricotta-type cheeses to control the food-borne pathogen Listeria monocytogenes. Letters in Applied Microbiology 24, 343-346. https://doi.org/10.1046/j.1472-765X.1997.00145.x PMid:9172439
  8. El Sheikh, M., Farrag, A., Zaghloul, A. (2010). Ricotta cheese from whey protein concentrate. Journal of American Sci. 6 (8):321-325.
  9. Bilyk, , Slyvka, N., Gutyj, B., Dronyk, H., Sukhorska, O. (2017). Study of the different ways  of proteins extraction from  sheep  and  cow  whey for Urda cheese production. Reports on research projects. Eureka: Life Sciences, Food Science and Technology. Number 3. https://doi.org/10.21303/2504-5695.2017.00333
  10. Dozet, N.D., Maćej, O.D., Jovanović, S.T. (2004). Indigenous dairy products as the basis for the development of specific original dairy products in modern conditions. Biotech. in Animal Husbandry 20 (3-4):31-48. https://doi.org/10.2298/BAH0404031D
  11. Kaminarides, S., Ilias Dimopoulos, E., Zoidou, E., Moatsou, G. (2015). The effect of the addition of skimmed milk on the characteristics of Myzithra cheeses. Food Chemistry 180, 164-170. https://doi.org/10.1016/j.foodchem.2015.01.086 PMid:25766814
  12. Antunac, N., Hudik, S., Mikulec, N., Maletić, M., Horvat, I., Radeljević, B., Havranek, J. (2011). Production and chemical composition of Istria and Pag whey cheese. Mljekarstvo 61 (4):326-335.
  13. Yasmin, A., Butt, S.M., Sameen, A., Shahid, M. (2013). Physicochemical and Amino Acid Profiling of Cheese Whey. Pakistan Journal of Nutrition 12 (5):455-459.
  14. Etzel, R.M. (2004). Manufacture and use of dairy protein fractions. The Journal of Nutrition 134 (4): 996-1002. https://doi.org/10.1093/jn/134.4.996S PMid:15051860
  15. Tratnik, Lj. (2003). The role of whey in the production of functional dairy Mljekarstvo 53 (4):325-352.
  16. Rozenberg, S., Body, J.J., Bruyere1, O., Bergmann, P., Brandi, M.L., Cooper, C., Devo-Gelaer, J.P., Gielen, E., Goemaerel, S., Kaufman, J.M., Rizzoli, R., Reginster, J.Y. (2016). Effects of dairy products consumption on health: benefits and beliefs-a commentary from the Belgian bone club and the European society for clinical and economic aspects of osteoporosis, osteoarthritis and musculoskeletal diseases. Calcif Tissue Int. 98, 1-17. https://doi.org/10.1007/s00223-015-0062-x PMid:26445771 PMCid:PMC4703621
  17. Cho, E., Smith Warner, S.A., Spiegelman, , Beeson, W.L., Van den Brandt, P.A., Colditz, G.A., Folsom, A.R., Fraser. G.E., Freudenheim, J.L., Giovannuci, E. (2004). Dairy foods, calcium, and colorectal cancer: a pooled analysis of 10 cohort studies. Journal of National Cancer Institute 96, 1015-1022. https://doi.org/10.1093/jnci/djh185 PMid:15240785
  18. Davoodi, , Esmaeili, S., Mortazavian, A.M. (2013). Effects of milk and milk products consumption on cancer: A Review. Comprehensive Reviews in Food Science and Food Safety 12, 249-264. https://doi.org/10.1111/1541-4337.12011
  19. Mateo, A.H., Macías, L., Romero, E.J., García, A.H., Blancas, L.A. (2012). Physiological effects beyond the significant gain in muscle mass in sarcopenic elderly men: evidence from a randomized clinical trial using a protein-rich food. Clinical Interventions in Aging (Albany NY) 7, 225-234. https://doi.org/10.2147/CIA.S32356 PMid:22866000 PMCid:PMC3410682
  20. Alichanidis, ,    Polychroniadou,    A.    (2008). Characteristics of major traditional regional cheese varieties of East-Mediterranean countries: a review. Dairy Science Technology 88, 495-510. https://doi.org/10.1051/dst:2008023
  21. AOAC (1995). Official Methods of Analysis of the AOAC, 16th ed. Methods 968.30, 18, 945.68, 981.12, Association of official analytical chemists, Arlington, VA, USA.
  22. AOAC (2005). Official Methods of  Analysis  of  the AOAC, 17th ed II, 33: Methods 926.08, 2000.18, 933.05, 2001.14, 935.42, 920.124, AOAC International, Gaithersburg, MD.
  23. IDF (2008). International IDF Standard 222:2008 (Cheese and processed cheese products. Determination of Fat Content-Van Gulik method), IDF, Brussels,
  24. AOAC (1998). Official  Methods  of   Analysis,  16th ed., 4th Revision. Methods 01, 984.15, 930.28, 972.16, AOAC International, Gaithersburg, MD.
  25. Henderson, W.J., Ricker, D.R., Bidlingmeyer, A.B., Woodward, C. (2000). Rapid, accurate, sensitive, and reproducible HPLC analysis of amino acid analysis using Zorbax Eclipse-AAA Columns and the Agilent 1100 HPLC. Agilent Technologies. PN 5980-1193E.
  26. Serbian Regulation (2014). Ordinance on the  quality of dairy products and starter Official Gazette of the Republic of Serbia, No. 34/2014.
  27. Chandan, C., Kilara, A. (2011). Dairy ingredients for food processing (pp. 179-199). Ames, IA: Willey Blackwell. https://doi.org/10.1002/9780470959169
  28. Vrdoljak, J., Dobranić, V., Filipović, I., Zdolec, N. (2016). Microbiological quality of soft, semi-hard and hard cheeses during the shelf-life. Mac Vet Rev. 39 (1):59-64.
  29. Borba, K.K.S. (2013). Development and characterization of creamy ricotta  cheese  made with rennet goat and bovine [dissertation]. Federal University of Paraiba, Campus I, Joao Pessoa, Paraiba.
  30. Casti, , Scarano, C., Pala, C., Cossu, F., Lamon, S., Spanu, V., Ibba, M., Mocci, A.M., Tedde, F., Nieddu, G., Spanu, S., De Santis, L.P.E. (2016).  Evolution  of the microbiological profile of vacuum-packed ricotta salata cheese during shelf-life. Italian Journal of Food Safety 5 (2): 57-60. https://doi.org/10.4081/ijfs.2016.5501 
  31. WHO/FAO (2011) [Internet]. Codex Milk and milk products. Second Edition. World Health Organization/ Food and Agriculture Organization of UN, Rome 83-85.
  32. Ribeiro, A.C., Marques, S.C., Sodré, F., Abreu, L.R., Piccoli,    R.H.        (2005).    Microbiological assessment of creamy ricotta during shelf-life. Science and Agrotechnology 29 (1):113-117. https://doi.org/10.1590/S1413-70542005000100014 
  33. Souza, M.R., Morais, F.A., Corrêa, C.E.S., Rodrigues, R. (2000). Physicochemical characteristics of ricotta commercialized in Belo Horizonte. MG. Rev Hig Aliment. 14, 68-71.
  34. Sulieman, A.M.E., Aisha ,  Eljack,  A.S.,  Zakaria, A., Salih, Z.A. (2012). Quality evaluation  of "Ricotta" cheese produced at laboratory level. International Journal of Food Science and Nutrition Engineering 2 (6):108-112. https://doi.org/10.5923/j.food.20120206.02
  35. Mattera, , Aguzzi, A. Nicoli, S. Cappelloni, M., Lucarini, M., Squasi, M.C., Marletta, L. (1999). Ewe and cow ricotta. An updating on nutrient composition. National Institute of Nutrition, Rome, Italy. [www.agris.fao.org/agris-search/search.do?recordID]
  36. Madalozzo, E.S., Sauer, E., Nagata, N. (2015). Determination of fat, protein and moisture in Ricotta cheese by near-infrared spectroscopy and multivariate calibration. Journal of Food Sci. and 52 (3):1649-1655. https://doi.org/10.1007/s13197-013-1147-z PMid:25745236 PMCid:PMC4348305
  37. Bojanic Rasovic, M., Nikolić, N., Rasovic, R. (2017). Quality of "Urda" obtained after production of Montenegrin semi-hard cheese. Food Research 1 (5):166-170.
  38. Pappa, C.E., Samelis, , Kondyli, E., Pappas, A. (2016). Characterisation of Urda whey cheese: Evolution of main biochemical and microbiological parameters during ripening and vacuum packaged cold storage. International Dairy Journal 58, 54-57. https://doi.org/10.1016/j.idairyj.2015.12.016
  39. Tomé, , Bos, C., Mariotti, F., Gaudichon, C. (2002). Protein quality and FAO/WHO recommendations. Food Sci. 22, 393-405. https://doi.org/10.3166/sda.22.393-405
  40. Wolfe, R. (2002). Regulation of muscle protein by amino acids. Journal of Nutrition 132, 3219-3224. https://doi.org/10.1093/jn/131.10.3219S PMid:12368421
  41. Valerio, A., D'Antona, G., Nisoli, E. (2011). Branched-chain amino     acids,      mitochondrial biogenesis, and healthspan: An evolutionary perspective. Aging (Albany NY) 3, 464-478. https://doi.org/10.18632/aging.100322 PMid:21566257 PMCid:PMC3156598
  42. Mohamed, S.A., Hasan, S.M., Abusalloum, T. (2014). Chemical characteristics of Italian Ricotta cheese as influenced by the proteolysis during ripening. Food Science and Technology Department, Fac. Mansoura Univ. 5 (2):45-53.
  43. Bilyk, (2011). Technological ensuring of production, processing of products of animal origin and their preservation. Scientific Messenger of  LNU Veterinary Medicine and Biotechnologies 2 (48): 317-321.
  44. Rako, A., Tudor Kalit, M., Kalit, S., Soldo, B., Ljubenkov, I. (2018). Nutritional characteristics of Croatian whey cheese (Bračka skuta) produced in different stages of lactation. LWT-Food Science and 96, 657-662. https://doi.org/10.1016/j.lwt.2018.06.024


© 2019 Paskaš S. This is an open-access article publishedunder the terms of the Creative Commons Attribution License whichpermits unrestricted use, distribution, and reproduction in any medium,provided the original author and source are credited.

Conflict of Interest Statement

The authors declared that they have no potential conlict of interest with respect to the authorship and/or publication of this article.

Citation Information

Macedonian Veterinary Review. Volume 42, Issue 2, Pages 151-161, e-ISSN 1857-7415, p-ISSN 1409-7621, DOI: 10.2478/macvetrev-2019-0020, 2019