Original Scientific Article
Assessment of antibody titer and lymphoid organs weight following Newcastle disease vaccination and feed-supplementation of vitamin-C, probiotics and antibiotic-growth-promoters in Japanese quails
Mirza Mienur Meher,
Nusrat Jahan,
Marya Afrin*

Mac Vet Rev 2021; 44 (2): 129 - 137

10.2478/macvetrev-2021-0016

Received: 22 October 2020

Received in revised form: 25 February 2021

Accepted: 26 February 2021

Available Online First: 17 May 2021

Published on: 15 October 2021

Correspondence: Marya Afrin, marya.afrin@bau.edu.bd

Abstract

Newcastle disease (ND) is a highly contagious viral disease. This study was conducted to determine the antibody titer in Japanese quails following vaccination against ND and feed-supplementation with vitamin-C, probiotics and antibioticgrowth- promoters. Forty Japanese quails were equally grouped in four groups (A1 to A4) and were vaccinated by LaSota, B1, F and VG/GA strains, respectively, at the age of 10 days. Another forty Japenese quails were equally grouped in four groups (B1 to B4), were vaccinated with the vaccines of the previous groups that showed a higher effect, and were fed with mesh-feed (control-B1), supplemented with vitamin-C (B2), probiotics (B3), and antibiotic growth promoter (B4). Serum samples were obtained on 2, 4, 6, 8, 10 and 12 weeks of age and were subjected to a hemagglutination inhibition test. The mean antibody titer (Log2) had no significant difference between groups A1-A4. The highest titers were recorded in A4 (6.20±0.37) and A3 (6.00±0.71). The mean titer was significantly different among the groups at 4 weeks, but insignificant in the other weeks of the supplemented groups. A higher mean titer was found between 8 and 10 weeks in group B2 (7.40±0.60) and B3 (7.20±0.58). The relative weights (gm/kg) of the spleen, thymus and bursa were significantly higher in group B3 (1.947±0.109, 5.951±0.312 and 2.624±0.083, respectively). They had no significant correlation with antibody titer at 12 weeks. In conclusion, vitamin-C and probiotic supplemented feeds can boost up the antibody titer against ND in Japanese quail when given during VG/GA strain vaccination.

Keywords: antibody, NDV, vaccination, vitamin C, probiotics, Japanese quail


References

  1. Onyewuchi, U.U., Offor, I.R., Okoli, C.F. (2013). Profitability of quail bird and egg production in IMO state, Nigeria. J Agric Food Environ. 9(1): 40-44.
  2. Susta, L., Segovia, D., Olivier, T.L., Dimitrov, K.M., Shittu, I., Marcano, V., Miller, P.J. (2018). Newcastle disease virus infection in quail. Vet Pathol. 55(5): 682-692. https://doi.org/10.1177/0300985818767996 PMid:29661124      
  3. Islam, M.A., Ito, T., Takakuwa, H., Takada, A., Itakura, C., Kida, H. (1994). Acquisition of pathogenicity of a Newcastle disease virus isolated from a Japanese quail by intracerebral passage in chickens. Jpn J Vet Res. 42(3-4): 147-156.       
  4. Merino, R., Villegas, H., Quintana, J.A., Calderon, N. (2009). Characterization of Newcastle disease viruses isolated from chicken, gamefowl, pigeon and quail in Mexico. Vet Res Commun. 33(8): 1023-1030. https://doi.org/10.1007/s11259-009-9321-5 PMid:19784791              
  5. De Leeuw, O., Peeters, B. (1999). Complete nucleotide sequence of Newcastle disease virus: Evidence for the existence of a new genus within the subfamily Paramyxovirinae. J Gen Virol. 80(1): 131-136. https://doi.org/10.1099/0022-1317-80-1-131 PMid:9934695       
  6. Alexander, D.J. (2000). Newcastle disease and other avian paramyxoviruses. Rev Sci Tech. 19(2): 443-462. https://doi.org/10.20506/rst.19.2.1231 PMid:10935273 
  7. OIE (2019). Manual of diagnostic tests and vaccines for terrestrial animals. Newcastle disease (infection with Newcastle disease virus) [Internet]. Section 3.3. Chapter 3.3.14. https://www.oie.int/fileadmin/Home/eng/Health_standards/tahm/3.03.14_NEWCASTLE_DIS.pdf
  8. Puthpongsiriporn, U., Scheideler, S.E., Sell, J.L., Beck, M.M. (2001). Effects of vitamin E and C supplementation on performance, in vitro lymphocyte proliferation, and antioxidant status of laying hens during heat stress. Poult Sci. 80(8): 1190-1200. https://doi.org/10.1093/ps/80.8.1190 PMid:11495472   
  9. Wu, C.C., Dorairajan, T., Lin, T.L. (2000). Effect of ascorbic acid supplementation on the immune response of chickens vaccinated and challenged with infectious bursal disease virus. Vet Immunol Immunopathol. 74(1-2): 145-152. https://doi.org/10.1016/S0165-2427(00)00161-6
  10. Aengwanich, W., Sridama, P., Phasuk, Y. Vongpralab, T., Pakdee, P., Katawatin, S., Simaraks, S. (2003). Effects of ascorbic acid on cell mediated, humoral immune response and pathophysiology of white blood cells in broilers under heat stress. Songklanakarin J Sci Technol. 23(3): 2297-305.
  11. Haghighi, H.R., Gong, J., Gyles, C.L., Hayes, M.A., Zhou, H., Sanei, B., Chambers J.R., Sharif, S. (2006). Probiotics stimulate production of natural antibodies in chickens. Clin Vaccine Immunol. 13(9): 975-980. https://doi.org/10.1128/CVI.00161-06 PMid:16960107 PMCid:PMC1563569       
  12. El-Shall, N.A., Awad, A.M., El-Hack, M.E.A., Naiel, M.A.E., Othman, S.I., Allam, A.A., Sedeik, M.E. (2019). The simultaneous administration of a probiotic or prebiotic with live salmonella vaccine improves growth performance and reduces fecal shedding of the bacterium in Salmonella-challenged broilers. Animals (Basel). 10(1): 70. https://doi.org/10.3390/ani10010070 PMid:31906020 PMCid:PMC7023486         
  13. Isolauri, E., Sütas, Y., Kankaanpää, P., Arvilommi, H., Salminen, S. (2001). Probiotics: Effects on immunity. Am J Clin Nutr. 73(2): 444s-450s. https://doi.org/10.1093/ajcn/73.2.444s PMid:11157355
  14. Lokapirnasari, W.P., Al Arif, A., Soeharsono, S., Fathinah, A., Najwan, R., Wardhani, H.C.P., et al. (2019). Improves in external and internal egg quality of Japanese quail (Coturnix coturnix japonica) by giving lactic acid bacteria as alternative antibiotic growth promoter. Iran J Microbiol. 11(5): 406-411.           
  15. Razee, A., Mahbub, A. S. M., Miah, M. Y., Hasnath, M. R., Hasan, M. K., Uddin, M. N., et al. (2016). Performance of Japanese quails (Coturnix coturnix japonica ) on floor and cage rearing system in Sylhet, Bangladesh: Comparative Study. Iran J Appl Anim Sci. 6(4): 931-936.      
  16. Khanam, J., Rahman, M.M., Fakhruzzaman, M., Rumi, N.A. (2018). Determination of efficacy of different Newcastle disease vaccine used in broiler chickens. Asian Australas J Biosci Biotechnol. 3(1): 52-58.
  17. Ambali, H.M., Nwoha, R.I.O., Abdu, P.A. (2017). Evaluation of antibody response to Newcastle disease vaccination in chickens in some commercial farms-Ii two local government areas in Lagos State, Nigeria. J Vet Med Surg. 1(2:10): 1-6. https://doi.org/10.9734/AJEA/2016/27456
  18. Barua, A.R., Amin, M.M., Islam, S., Chowdhury, S., Khan, M.S.I., Asgar, M.A. (2008). Evaluation of antibody production against Newcastle disease virus after immunization with different vaccines in Fayoumi chicks. Bangladesh J Microbiol. 25(1):31-35. https://doi.org/10.3329/bjm.v25i1.4852
  19. Meher, M.M., Rahman, M.M., Akter, M.R., Rahaman, M.S., Rahman, M.K. (2017). Detection of avian infectious bronchitis virus and its specific antibody in different ages layer birds in Dinajpur district of Bangladesh. Int J Res Appl Sci Eng Technol. 5(11): 2502-2507. https://doi.org/10.22214/ijraset.2017.11350
  20. Allan, W.H., Gough, R.H. (1974). A standard hemagglutination inhibition test for Newcastle disease. (1). A comparison of macro and micromethods. Vet Rec. 95(6): 120-123. https://doi.org/10.1136/vr.95.6.120 PMid:4446306         
  21. Brugh, M.G. (1978). A simple method for recording and analyzing serological data. Avian Dis. 22(2): 362-365. https://doi.org/10.2307/1589552 PMid:678239  
  22. Mahmud, M.S., Hossain, M.T., Monoura, P., Amin, M.M. (2007). Comparative efficacy of avinew (VG/GA strain) and BCRDV (F strain) vaccines against Newcastle disease in broiler chickens. Bangladesh J Vet Med. 5(1-2): 19-23. https://doi.org/10.3329/bjvm.v5i1.1304
  23. Borland, L.J., Allan, W.H. (1980). Laboratory tests for comparing live lentogenic newcastle disease vaccines. Avian Pathol. 9(1): 45-59. https://doi.org/10.1080/03079458008418385 PMid:18770239
  24. Ibrahim, A.L., Lai, M.C., Aini, I. (1983). Spray vaccination with an improved F Newcastle disease vaccine. A comparison of efficacy with the B1 and La Sota vaccines. Br Vet J. 139(3): 213-219. https://doi.org/10.1016/S0007-1935(17)30486-4
  25. Rahman, M.B., Rahman, M.M., Rahman, M., Kabir, S.M.L., Nazir, K.H.M.N.H., Amin, M.M. (2004). Efficacy of V4HR newcastle disease (V4HR-ND) vaccine in broiler birds in Bangladesh. Int J Poult Sci. 3(5): 365-368. https://doi.org/10.3923/ijps.2004.365.368
  26. Abdel Rhman, S.S., Al Jassem, A.H., Hussein, G.M., Al-Blowi, M.H. (2013). Comparison between haemagglutination inhibition test and enzyme linked immune sorbent assay in evaluation of Newcastle disease antibodies in Japanese quails. J World's Poult Res. 3(3): 83-88.
  27. Bhatti, N., Hussain, Z., Mukhtar, M., Ali, A., Imran, M., Rafique, A., Manzoor, S., Rehman, S. (2016). Effects of vitamins E and C supplementation on the immune response of broiler chicks. J Antivir Antiretrovir. 8(4): 151-154.
  28. Talebi, A., Amirzadeh, B., Mokhtari, B., Gahri, H. (2008). Effects of a multi-strain probiotic (PrimaLac) on performance and antibody responses to Newcastle disease virus and infectious bursal disease virus vaccination in broiler chickens. Avian Pathol. 37(5): 509-512. https://doi.org/10.1080/03079450802356995 PMid:18798026   
  29. Elagib, H.A.A., Omer, H.M. (2012). Effect of dietary ascorbic acid on performance and immune response of heat stressed broiler chicks. Pakistan J Nutr. 11(3): 216-220. https://doi.org/10.3923/pjn.2012.216.220
  30. Huang, Z., Elankumaran, S., Yunus, A.S., Samal, S.K. (2004). A recombinant Newcastle disease virus (NDV) expressing VP2 protein of infectious bursal disease virus (IBDV) protects against NDV and IBDV. J Virol. 78(18): 10054-10063. https://doi.org/10.1128/JVI.78.18.10054-10063.2004 PMid:15331738 PMCid:PMC514986               
  31. Maassen, C.B.M., Van Holten-Neelen, C., Balk, F., Den Bak-Glashouwer, M. J., Leer, R.J., Laman, J.D., Boersma, W.J., Claassen, E. (2000). Strain-dependent induction of cytokine profiles in the gut by orally administered Lactobacillus strains. Vaccine. 18(23): 2613-2623. https://doi.org/10.1016/S0264-410X(99)00378-3
  32. Cheng, Y.H., Lee, D.N., Wen, C.M., Weng, C.F. (2004). Effects of β-glucan supplementation on lymphocyte proliferation, macrophage chemotaxis and specific immune responses in broilers. Asian-Australas J Anim Sci. 17(8): 1145-1149. https://doi.org/10.5713/ajas.2004.1145
  33. Christensen, H.R., Frøkiær, H., Pestka, J.J. (2002). Lactobacilli differentially modulate expression of cytokines and maturation surface markers in murine dendritic cells. J Immunol. 168(1): 171-178. https://doi.org/10.4049/jimmunol.168.1.171 PMid:11751960   
  34. Bai, A.P., Ouyang, Q., Zhang, W., Wang, C.H., Li, S.F. (2004). Probiotics inhibit TNF-α-induced interleukin-8 secretion of HT29 cells. World J Gastroenterol. 10(3): 455-457. https://doi.org/10.3748/wjg.v10.i3.455 PMid:14760780 PMCid:PMC4724907       
  35. Willis, W.L., Isikhuemhen, O.S., Ibrahim, S.A. (2007). Performance assessment of broiler chickens given mushroom extract alone or in combination with probiotics. Poult Sci. 86(9): 1856-1860. https://doi.org/10.1093/ps/86.9.1856 PMid:17704371           
  36. Khan, S.H., Yousaf, B., Mian, A.A., Rehman, A., Farooq, M.S. (2011). Assessing the effect of administering different probiotics in drinking water supplement on broiler performance, blood biochemistry and immune response. J Appl Anim Res. 39(4): 418-428. https://doi.org/10.1080/09712119.2011.623783
  37. Kumar, M.V., Patil, V.M., Kiran, M., Tendulkar, S.M. (2018). Effect of dietary supplementation of probiotics (Addon Poultry Max) on biochemical and immune parameters in commercial broiler chicken. Int J Curr Microbiol Appl Sci. 7(10): 1537-1542. https://doi.org/10.20546/ijcmas.2018.710.171


Copyright

© 2021 Meher M.M. This is an open-access article published under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Conflict of Interest Statement

The authors have declared that no competing interests exist.

Citation Information

Macedonian Veterinary Review. Volume 44, Issue 2, Pages 129-137, e-ISSN 1857-7415, p-ISSN 1409-7621, DOI: 10.2478/macvetrev-2021-0016, 2021